LOCUS       sequence-464618-        8976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible red light-inducible Flp-N; Flp-N396-FHY1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8976)
  AUTHORS   Tous C, Kinstlinger IS, Rice MEL, Deng J, Wong WW
  TITLE     Multiplexing light-inducible recombinases to control cell fate, 
            Boolean logic, and cell patterning in mammalian cells.
  JOURNAL   Sci Adv. 2025 May 9;11(19):eadt1971. doi: 10.1126/sciadv.adt1971. 
            Epub 2025 May 9.
  PUBMED    40344052
REFERENCE   2  (bases 1 to 8976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-05-9"; volume: "11(19):eadt1971. doi"; pages: " 
            10.1126/sciadv.adt1971. Epub 2025 May 9"
COMMENT     Sequence Label: IK350
FEATURES             Location/Qualifiers
     source          1..8976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(935..955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    997..1118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1034..1053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1088..1107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   1881..1915
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(2406..2422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2406..2422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2419..2441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2430..2446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2454..2460,2461..2478,2479..2484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2499..2520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2637..2654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2808..3396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2888..2907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3567..4358,4359..4427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4190..4209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4428..4532)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4558..5013)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4695..4716)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4907..4926
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5140..5162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5154..5171
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5155..5171
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5301..5363
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        5818..6196
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    5826..5844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5862..5880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5898..5916
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5934..5952
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5970..5988
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6006..6024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6042..6060
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6105..6129
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             8051..8071
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8081..8305
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8443..8463)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8448..8777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8628..8763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8690..8709
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(8826..8976,1..875)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(8826..8976,1..875)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
       61 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
      121 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
      181 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
      241 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
      301 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
      361 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
      421 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
      481 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
      541 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
      601 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
      661 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
      721 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
      781 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
      841 gacgccccag cactcgtccg agggcaaagg aataggtcga caatcaacct ctggattaca
      901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
      961 acgctgcttt aatgcctttg tatcatgcgt taactaaact tgtttattgc agcttataat
     1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggaa ttgactcaaa
     1141 tgatgtcaat tagtctatca gaagctcatc tggtctccct tccgggggac aagacatccc
     1201 tgtttaatat ttaaacagca gtgttcccaa actgggttct tatatccctt gctctggtca
     1261 accaggttgc agggtttcct gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc
     1321 caggtttagc cccggaattg actggattcc ttttttaggg cccattggta tggctttttc
     1381 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     1441 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     1501 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
     1561 agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa
     1621 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     1681 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     1741 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     1801 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     1861 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat
     1921 ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
     1981 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
     2041 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
     2101 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
     2161 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
     2221 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
     2281 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
     2341 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa
     2401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2641 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2701 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     4561 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4621 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4681 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4741 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4801 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4861 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4921 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4981 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     5041 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     5101 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     5161 cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag gacgggcaga
     5221 ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc gacacgctgc
     5281 agaacacgca gctagattaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
     5341 atcatgtgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
     5401 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
     5461 acaatttatt tatgtttatt tatttattaa aaaaaacaaa aactcaaaat ttcttctata
     5521 aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga tgtaattacg
     5581 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     5641 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     5701 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     5761 acggggaaaa ggcctccacg gccactagtg aggccctttc gtcttcaaga attcctcgag
     5821 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
     5881 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
     5941 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
     6001 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
     6061 acgtataagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac
     6121 cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
     6181 ttcctaccct cgtaaagtcg acacgcgtgc cgccaccatg agccagttcg acatcctgtg
     6241 caagaccccc cccaaggtgc tggtgcggca gttcgtggag agattcgaga ggcccagcgg
     6301 cgagaagatc gccagctgtg ccgccgagct gacctacctg tgctggatga tcacccacaa
     6361 cggcaccgcc atcaagaggg ccaccttcat gagctacaac accatcatca gcaacagcct
     6421 gagcttcgac atcgtgaaca agagcctgca gttcaagtac aagacccaga aggccaccat
     6481 cctggaggcc agcctgaaga agctgatccc cgcctgggag ttcaccatca tcccttacaa
     6541 cggccagaag caccagagcg acatcaccga catcgtgtcc agcctgcagc tgcagttcga
     6601 gagcagcgag gaggccgaca agggcaacag ccacagcaag aagatgctga aggccctgct
     6661 gtccgagggc gagagcatct gggagatcac cgagaagatc ctgaacagct tcgagtacac
     6721 cagcaggttc accaagacca agaccctgta ccagttcctg ttcctggcca cattcatcaa
     6781 ctgcggcagg ttcagcgaca tcaagaacgt ggaccccaag agcttcaagc tggtgcagaa
     6841 caagtacctg ggcgtgatca ttcagtgcct ggtgaccgag accaagacaa gcgtgtccag
     6901 gcacatctac tttttcagcg ccagaggcag gatcgacccc ctggtgtacc tggacgagtt
     6961 cctgaggaac agcgagcccg tgctgaagag agtgaacagg accggcaaca gcagcagcaa
     7021 caagcaggag taccagctgc tgaaggacaa cctggtgcgc agctacaaca aggccctgaa
     7081 gaagaacgcc ccctacccca tcttcgctat caagaacggc cctaagagcc acatcggcag
     7141 gcacctgatg accagctttc tgagcatgaa gggcctgacc gagctgacaa acgtggtggg
     7201 caactggagc gacaagaggg cctccgccgt ggccaggacc acctacaccc accagatcac
     7261 cgccatcccc gaccactact tcgccctggt gtccaggtac tacgcctacg accccatcag
     7321 caaggagatg atcgccctga aggacgagac caaccccatc gaggagtggc agcacatcga
     7381 gcagctgaag ggcagcgccg agggctccgg agggtctggc tccggatcaa gtggtggcag
     7441 cggtaccccc gaggtggagg tcgacaacaa caacgagaag cccagcgaga tcaacagctt
     7501 ccaccacatg attatcagca gcagcaagaa cgtgctgaag atggaggagg tggaggtgtc
     7561 caagaagagg aagttccaga ccgaccagag cgacgagctg agcttattac ctttatccaa
     7621 acacacatgc tttgccaacg tggcttgtag cgagaacacc aacggcaaca gcgagatcga
     7681 taccgagtac agcatgtcca gctacgtgaa ctccaccacc tccatggagt gcaacaacga
     7741 catcgagatg aaggaagaat ccagcggcag ctgcggagag gacaagatga tcagcttcga
     7801 gtcccattta gactatatct acggcaccca gaatctggag gacttctccg agaaggtgat
     7861 cgagaacatt ctgtacctcg acgagcaaga agaggaggag gaggacgcca agggctgtag
     7921 cagcaacgct gccaagttcg tgctgtcctc tggtcgttgg accgtgaacc aagatgatag
     7981 cactttacac gaaaccaaga agcccaccat cgaccaagaa ttcgagcagt actttagcac
     8041 tttaatgctg cccaagaaaa agcggaaggt gtgagaattc ctgtgccttc tagttgccag
     8101 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8161 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8221 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8281 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     8341 gggtatcccc acgcgccctg tagcgtgtac cgctgggagt tcgtagacgg aaacaaacgc
     8401 agaatccaag cgcggccgca atttaacgcg aattaattct gtggaatgtg tgtcagttag
     8461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8701 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8761 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     8821 acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     8881 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     8941 tcgatgtagg agggcgtgga tatgtcctgc gggtaa
//
