LOCUS       sequence-464624-        9938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BFP-tagged ARF6 WT for Turbo ID experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Nikolatou K, Sandilands E, Roman-Fernandez A, Cumming EM, Freckmann 
            E, Lilla S, Buetow L, McGarry L, Neilson M, Shaw R, Strachan D, 
            Miller C, Huang DT, McNeish IA, Norman JC, Zanivan S, Bryant DM
  TITLE     PTEN deficiency exposes a requirement for an ARF GTPase module for 
            integrin-dependent invasion in ovarian cancer.
  JOURNAL   EMBO J. 2023 Sep 18;42(18):e113987. doi: 10.15252/embj.2023113987. 
            Epub 2023 Aug 14.
  PUBMED    37577760
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-09-18"; volume: "42(18):e113987. doi"; pages: " 
            10.15252/embj.2023113987. Epub 2023 Aug 14"
COMMENT     Sequence Label: hARF6-R WT TurboID V5 T2A BFP
FEATURES             Location/Qualifiers
     source          1..9938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      601..610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1156..2112
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2119..2160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2167..2220
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2221..2922
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2945..3533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2998..3018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3416..3427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3605..3838
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3916..4037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3953..3972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4007..4026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4077..4212
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4232..4251)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4233..4251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4261..4278)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4261..4277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4270..4292)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4419..4874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4506..4525)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4716..4737
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4900..5004
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5005..5073,5074..5865)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6036..6624
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6525..6544
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6778..6795
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6912..6933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6948..6953,6954..6971,6972..6978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6986..7002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6991..7013
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7010..7026
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7010..7026
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7045..7065
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7047..7065
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7093..7319
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7320..7500
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7547..7672
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8165..8398
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8583..8627
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8802..9302
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9221..9239
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9314..9709
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9769..9886
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 gccaccatgg ggaaggtgct atccaaaatc ttcgggaaca aggaaatgcg gatcctcatg
      661 ttgggcctgg acgcggccgg caagacaaca atcctgtaca agttgaagct gggccagtcg
      721 gtgaccacca ttcccactgt gggtttcaac gtggagacgg tgacttacaa aaatgtcaag
      781 ttcaacgtat gggatgtggg cggccaggac aagatccggc cgctctggcg gcattactac
      841 actgggaccc aaggtctcat cttcgtagtg gactgcgccg accgcgaccg catcgatgag
      901 gctcgccagg agctgcaccg cattatcaat gaccgggaga tgagggacgc cataatcctc
      961 atcttcgcca acaagcagga cctgcccgat gccatgaaac cccacgagat ccaggagaaa
     1021 ctgggcctga cccggattcg ggacaggaac tggtatgtgc agccctcctg tgccacctca
     1081 ggggacggac tctatgaggg cctgacctgg ctgaccagca actacaaatc tggatccgga
     1141 tcaggaagca tcgataaaga caatactgtg cctctgaagc tgatcgctct cctggctaat
     1201 ggcgagttcc atagtggcga acagctggga gaaaccctgg gcatgtccag ggccgctatc
     1261 aacaagcaca ttcagactct gcgcgactgg ggcgtggacg tgttcaccgt gcccggaaag
     1321 ggctactctc tgcccgagcc tatcccgctg ctgaacgcta aacagattct gggacagctg
     1381 gacggcggga gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat
     1441 cgaatcggcg agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga
     1501 ggaagcagag ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt
     1561 tggcgcctga agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg
     1621 gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg
     1681 tatctgcagg atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat
     1741 gctgcacaga tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc
     1801 gtggtcaatc agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact
     1861 ctggccgcta cgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc
     1921 ctggctccat atctgccacg gtgggagaag ctggataact tcatcaatag acccgtgaag
     1981 ctgatcattg gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc
     2041 ctgctgctgg aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg
     2101 tctgccgaaa agcgtacggg caagcccatc cccaaccccc tgctgggcct ggacagcacc
     2161 acgcgtgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
     2221 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     2281 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     2341 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     2401 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     2461 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     2521 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     2581 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     2641 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     2701 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     2761 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     2821 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     2881 tactgcgacc tccctagcaa actggggcac aagcttaatt aaaccggtgg cgcgttaagt
     2941 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3001 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3061 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3121 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3181 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3241 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3301 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3361 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3421 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3481 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     3541 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     3601 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     3661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3841 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     3901 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4021 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     4081 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4141 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4201 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     4261 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4321 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4381 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4441 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4501 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4561 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4621 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4681 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4741 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4801 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4861 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4921 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4981 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5041 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5101 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5161 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5221 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5281 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5341 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5401 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5461 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5521 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5581 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5641 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5701 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5761 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5821 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5881 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5941 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6001 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6061 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6121 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6181 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6241 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6301 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6361 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6421 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6481 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6541 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6601 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6661 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6721 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6781 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6841 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6901 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6961 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7021 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     7081 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     7141 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     7201 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     7261 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     7321 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7381 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7441 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7501 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     7561 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7621 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     7681 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     7741 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     7801 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     7861 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     7921 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7981 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     8041 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8101 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     8161 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     8221 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     8281 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8341 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8401 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8461 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     8521 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     8581 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     8641 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     8701 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     8761 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cttgcgcctt ttccaaggca
     8821 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     8881 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     8941 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     9001 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     9061 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     9121 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     9181 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     9241 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     9301 agggggtacc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     9361 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     9421 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     9481 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     9541 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     9601 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     9661 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc tgcagctgca
     9721 gtagtaagaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     9781 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     9841 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9901 ggacagcaga gatccacttt ggcgccggct cgaggggg
//
