LOCUS       sequence-464626-       10088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To Express HA tagged DjCas13d the CMV promoter. Also encodes a 
            mCherry gene outside of the viral genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Back F, Sandoval A, Vu LM, Hong VM, Bhaskara A, Rodriguez SR, 
            O'Brien JT, Kolber BJ, Kroener S, Ploski JE
  TITLE     Adeno-associated viral vector resource for the RNA-targeting Cas13d:
            A comparison of high-fidelity variants, DjCas13d and hfCas13d.
  JOURNAL   Mol Ther Methods Clin Dev. 2025 Aug 20;33(4):101565. doi: 
            10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11.
  PUBMED    41020266
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2025-08-20"; volume: "33(4):101565. doi";
            pages: " 10.1016/j.omtm.2025.101565. eCollection 2025 Dec 11"
COMMENT     Sequence Label: pAAV-CMV-intron-DjCas13d-pA/EF1a-mCherry-WPRE-pa
FEATURES             Location/Qualifiers
     source          1..10088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     enhancer        856..1159
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1160..1363
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1314..1334
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1360..1384
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1512..1890
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             1988..2008
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4658..4678
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4688..4714
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4729..4777
                     /note="synthetic polyadenylation signal"
     repeat_region   4794..4934
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4794..4923
                     /label=AAV2 ITR
     promoter        4958..6136
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5189..6127
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6084..6104
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6149..6856
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6291..6309)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6559..6578
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7028..7616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7081..7101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7499..7510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7648..8124
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(7847..7866)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      8216..8671
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8303..8322)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8513..8534
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8688..8707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8807..8829
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8867..8885)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8953..9057
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9058..9126,9127..9918)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9276..9295)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtctagtt attaatagta atcaattacg gggtcattag ttcatagccc
      841 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      901 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      961 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1021 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1081 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1141 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1201 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1261 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1321 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1381 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1441 cagcctccgc ggattcgaat cccggccggg aacggtgcat tggaacgcgg attccccgtg
     1501 ccaagagtga cgtaagtacc gcctatagag tctataggcc cacaaaaaat gctttcttct
     1561 tttaatatac ttttttgttt atcttatttc taatactttc cctaatctct ttctttcagg
     1621 gcaataatga tacaatgtat catgcctctt tgcaccattc taaagaataa cagtgataat
     1681 ttctgggtta aggcaatagc aatatttctg catataaata tttctgcata taaattgtaa
     1741 ctgatgtaag aggtttcata ttgctaatag cagctacaat ccagctacca ttctgctttt
     1801 attttatggt tgggataagg ctggattatt ctgagtccaa gctaggccct tttgctaatc
     1861 atgttcatac ctcttatctt cctcccacag ctcctgggca acgtgctggt ctgtgtgctg
     1921 gcccatcact ttggcaaaga attgggattc gaacatcgat cggaccggtg ctagcgccac
     1981 catgagcccc aagaagaaga gaaaggtgga ggccagcatc aagaagcaaa aatccaagaa
     2041 aacggtttct aaaacctccg gattgaaaga ggcactttct gtgcagggga ctgtcataat
     2101 gacgtctttt gggaagggga acatggcgaa tctctcttac aagatcccga gctctcaaaa
     2161 gccccaaaat ctcaatagta gcgccggctt gaagaacgtc gaagtatctg gtaaaaagat
     2221 taagttccaa ggtcgacacc cgaagatcgc cactaccgac aaccccctgt ttaagccgca
     2281 gccaggtatg gaccttctgt gcctgaaaga caaactcgaa atgcactatt ttgggaagac
     2341 tttcgacgac aatatccaca tacaattgat atatcaaata ctggatattg aaaagatcct
     2401 tgcggttcat gttaacaaca tcgtattcac actggacaat gtcctgcatc cgcaaaagga
     2461 agagctgact gaagatttca ttggggcagg gggctggcgg attaatctcg attaccaaac
     2521 tcttagaggg cagactaaca aatatgatag gtttaaaaac tacattaaaa gaaaagaact
     2581 tctttatttc ggggaggcat tttaccatga aaacgagaga cggtatgagg aagacatctt
     2641 cgcaatactt accctgctgt ctgcccttag acagttttgc tttcattccg atctgagtag
     2701 tgatgagagc gaccacgtga attcattctg gctgtatcaa ctcgaggacc aactgtctga
     2761 cgagttcaag gagactctca gtattttgtg ggaagaggtc accgaaagga tagatagtga
     2821 attccttaaa actaatacag taaacctcca tattttgtgt catgttttcc cgaaagagag
     2881 taaggagacg attgtacgcg cttattatga attcctgatc aagaagtcct ttaaaaatat
     2941 gggatttagc atcaagaaat tgcgagagat tatgctcgag caatcagatc ttaagagttt
     3001 caaagaggat aagtacaact ccgtcagggc taaactgtat aagctcttcg actttattat
     3061 aacctattac tacgaccacc atgcttttga gaaggaggcg ctcgtgagct ccttgcgaag
     3121 ttcactcact gaggaaaaca aggaagagat ttacatcaaa acggcccgga cactcgcgag
     3181 cgctcttggg gccgatttca agaaagccgc tgccgatgta aatgccaaaa acataaggga
     3241 ctatcagaag aaggcgaacg actataggat aagttttgaa gatattaaaa tagggaacac
     3301 aggcattggg tatttctcag agctcatata catgctcacc cttctcctcg atggaaagga
     3361 gataaacgac ctcctcacaa ccctgatcaa caaatttgat aatataatct ccttcataga
     3421 catcctgaag aaactcaatt tggaatttaa attcaagcct gaatacgcgg acttctttaa
     3481 catgaccaac tgcaggtata ctctcgaaga gctgcgggtt atcaacagta tcgctagaat
     3541 gcaaaagcca agcgccgacg cccgaaagat catgtatcgc gatgctctgc gcatattggg
     3601 aatggacaat cggccggatg aagagattga tcgggaactc gaaaggacaa tgcccgtggg
     3661 tgcggatggc aaatttatca aaggtaaaca gggctttcgc aatttcattg cttccaacgt
     3721 tattgaatct tcacgcttcc attacctcgt acggtataat aatccacaca agacacggac
     3781 tcttgtgaaa aaccctaatg tagtaaaatt tgtgctcgaa gggattccgg agacacaaat
     3841 aaaacgatac tttgatgttt gtaaaggtca agagatccca ccgacgtctg acaagtccgc
     3901 acagatagac gttcttgccc gaataattag ttcagtagac tataaaatat ttgaagacgt
     3961 accccaaagt gcaaagatca ataaagatga tcctagccga aacttcagtg acgcgctgaa
     4021 aaaacagcgg taccaggcaa ttgtaagtct ttatttgact gttatgtacc ttataaccaa
     4081 aaacctggtg tatgtcaata gtaggtatgt gattgcattt cattgcctgg aacgagacgc
     4141 attccttcac ggtgtgacgc tccctaagat gaataaaaaa attgtgtaca gtcaattgac
     4201 tactcacctc ttgactgaca aaaattacac tacgtacggc cacctgaaga accagaaagg
     4261 acaccgcaaa tggtacgttc ttgtcaaaaa taaccttcag aatagcgaca taacagctgt
     4321 gagttcattc cgcaacatag ttgctcacat atccgttgtg aggaatagta atgaatacat
     4381 aagcgggatc ggagaactgc actcttactt cgaactttat cactatctcg ttcaatcaat
     4441 gatcgcaaag aataattggt atgataccag ccaccagccg aagaccgccg aatatctgaa
     4501 caatctgaaa aagcaccaca cttattgtaa ggactttgta aaagcgtact gtatcccgtt
     4561 cggatacgtc gtgccgcggt acaaaaacct cacgataaat gaactttttg atcgaaataa
     4621 cccgaatccc gaaccaaaag aggaagtggg atccggacct aagaaaaaga ggaaggtggc
     4681 ggccgcttac ccatacgatg ttccagatta cgcttaggct aggaattcaa taaaagatct
     4741 ttattttcat tagatctgtg tgttggtttt ttgtgtgcgg accgagcggc cgcaggaacc
     4801 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4861 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4921 cagctgcctg caggggcgca ccggtgcatg cactagtggc tccggtgccc gtcagtgggc
     4981 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     5041 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5101 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5161 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     5221 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     5281 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     5341 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     5401 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     5461 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     5521 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     5581 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     5641 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     5701 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     5761 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     5821 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     5881 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     5941 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     6001 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     6061 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     6121 atttcaggtg tcgtgaggta ccggatccgt gagcaagggc gaggaggata acatggccat
     6181 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     6241 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     6301 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     6361 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     6421 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     6481 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     6541 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     6601 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     6661 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     6721 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     6781 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     6841 cgagctgtac aagtaaggcg cgcccccccc taacgttact ggccgaagcc gcttggaata
     6901 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6961 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaaattcg atatcaagct
     7021 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7081 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7141 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7201 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7261 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7321 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     7381 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7441 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7501 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7561 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     7621 ataccgagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct
     7681 ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt
     7741 tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta
     7801 tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca
     7861 agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg
     7921 attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc
     7981 taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac
     8041 tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga
     8101 accactgctc ccttccctgt ccttactagt gcatgcaccg gtgcgcctga tgcggtattt
     8161 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     8221 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8281 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8341 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8401 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     8461 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8521 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
     8581 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8641 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     8701 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8761 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8821 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     8881 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8941 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9001 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9061 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9121 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     9181 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     9241 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9301 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9361 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9421 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9481 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9541 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9601 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9661 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9721 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9781 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9841 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9901 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9961 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10081 ggatcttc
//
