LOCUS       sequence-464630-       10074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses LaccID on the mammalian cell surface, dox-inducible 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Lee SY, Roh H, Gonzalez-Perez D, Mackey MR, Hoces D, McLaughlin CN, 
            Lin C, Adams SR, Nguyen K, Kim KY, Luginbuhl DJ, Luo L, Udeshi ND, 
            Carr SA, Hernandez-Lopez RA, Ellisman MH, Alcalde M, Ting AY
  TITLE     Directed evolution of LaccID for cell surface proximity labeling and
            electron microscopy.
  JOURNAL   Nat Chem Biol. 2025 Aug 1. doi: 10.1038/s41589-025-01973-6.
  PUBMED    40751001
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Aug 1. doi: 10.1038/s41589-025-01973-6."
COMMENT     Sequence Label: pTRE3G_SS-HA-V5-LaccID-CD4 TM_Puro
FEATURES             Location/Qualifiers
     source          1..10074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1752..1869
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1999..2017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2035..2053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2107..2125
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2143..2161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2308..2317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     2314..2373
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             2377..2403
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2431..2472
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4685..5273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4738..4758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5156..5167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5293..5803
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5722..5740
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5813..6556
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6566..6622
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6623..7219
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7116..7136
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7318..7551
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7623..7744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7660..7679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7714..7733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7784..7919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7919..7990
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7991..8059,8060..8851)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8209..8228)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9022..9610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9511..9530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9764..9781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9898..9919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9934..9939,9940..9957,9958..9964)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9972..9988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9977..9999
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9996..10012
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9996..10012
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10031..10051
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10033..10051
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag aggatccgcc accatggaga cagacacact cctgctatgg
     2341 gtactgctgc tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat
     2401 gctggggccc agccggccag atctggcggc ggcaagccca tccccaaccc cctgctgggc
     2461 ctggacagca ccgctagcgc gataggacct gtggcagacc ttcacatcgc caatgcagac
     2521 atcagcccgg atgggttcac tcgttccgct gtgctagcgg gtggaacatt tcccggtccc
     2581 ttaataacgg gtaataaagg agataatttc cggataaacg tcatcaacca gcttacggac
     2641 acgactatgt tgaggtcaac cagtatacat tggcacggat tctttcaaca cgggacaagt
     2701 tgggctgacg gccccgcttt cgtcactcag tgccccatcg caccgggtaa cagttttctt
     2761 tacaacttca ctgtcccgga tcaagcgggc acgttctggt atcatagtca cctaagtacc
     2821 cagtattgtg acggacttag tggtccgttt gtagtttacg accccaacga tccccatcgt
     2881 agcttatacg atgtggatga tgaaagcact gtaattactc ttgcggactg gtaccacacc
     2941 ccggctcccc agttggccgc tgcgccgccc gtcccagata gcaccctaat aaacgggaaa
     3001 ggaaggtatg ccggaggccc aacgtccccg ttagccgtga taaacgtaga gcagggaaaa
     3061 agatataggt ttcgtcttat atctatttcc tgtgatccga attttacatt tagcattgat
     3121 ggccataata tgactatcat tgaagctgat ggggtgaaca cgcaaccact gaccgtggac
     3181 tctatccaga ttttcgctgg ccaaagatat tccttcgtcc tgaatgcgaa ccagcctgtt
     3241 ggtaactatt ggataagagc gaatccaaat ataggaacga caggattcgc aggtggtatt
     3301 aatagtgcga tcctacgtta tgtaggagct ccagaagcag accctacaac gacccaaaca
     3361 acatccacaa atccagtgaa cgaaacaaac ctacacccct tggagaaccc cggagccccg
     3421 gggaatccga ctccgggtgg tgctgatgtg aacatcaacc ttaacatcgg attctcctca
     3481 acgggacaat tcaccataaa cggtgtttcc ttcataccac ccaccgttcc cgtcttgttg
     3541 caaatcctgt ccggggcgcg tacggcacaa gatctgcttc ctagcggctc tgtttataca
     3601 ttaccgccga acaaagtcat agaaattagt atgccaggcg gcgctagcgc agccgggggg
     3661 cctcacccgt tccatttgca cggccacact tttgatgtgg tacgttcagc gggatcatca
     3721 acttacaact acgtgaatcc agtcagaaga gatgtcgtct ccattggaac ggcgggagat
     3781 aacgtgacaa ttaggttcac tacggacaac ccgggtccat ggttcctaca ttgtcatatc
     3841 gactggcatc ttgaggccgg tttagccgtc gtattcgctg aagacacacc tgatatcgca
     3901 agcgctaatc ccgttccggc tgcgtgggac gacctatgcc ctatttatga tgcgttaagc
     3961 cccgacgatc aaaccggtct gcaggtcgac ctcgacttcc agaaggcctc cagcatagtc
     4021 tataagaaag agggggaaca ggtggagttc tccttcccac tcgcctttac agttgaaaag
     4081 ctgacgggca gtggcgagct gtggtggcag gcggagaggg cttcctcctc caagtcttgg
     4141 atcacctttg acctgaagaa caaggaagtg tctgtaaaac gggttaccca ggaccctaag
     4201 ctccagatgg gcaagaagct cccgctccac ctcaccctgc cccaggcctt gcctcagtat
     4261 gctggctctg gaaacctcac cctggccctt gaagcgaaaa caggaaagtt gcatcaggaa
     4321 gtgaacctgg tggtgatgag agccactcag ctccagaaaa atttgacctg tgaggtgtgg
     4381 ggacccacct cccctaagct gatgctgagc ttgaaactgg agaacaagga ggcaaaggtc
     4441 tcgaagcggg agaaggcggt gtgggtgctg aaccctgagg cggggatgtg gcagtgtctg
     4501 ctgagtgact cgggacaggt cctgctggaa tccaacatca aggttctgcc cacatggtcc
     4561 accccggtgc agccaatggc cctgattgtg ctggggggcg tcgccggcct cctgcttttc
     4621 attgggctag gcatcttctt ctgtgtcagg tgccggcacc gaaggcgcta atagggccga
     4681 attcaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4741 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4801 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4861 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4921 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4981 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5041 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5101 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5161 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5221 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtctg
     5281 cagatatcat ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     5341 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     5401 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     5461 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     5521 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     5581 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     5641 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     5701 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     5761 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgtctag
     5821 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga
     5881 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5941 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     6001 tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     6061 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     6121 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     6181 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     6241 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     6301 cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga
     6361 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     6421 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     6481 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     6541 tgacatgctc cccgggggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     6601 cgtggaggag aaccctggac ctaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6661 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6721 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6781 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6841 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6901 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6961 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7021 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7081 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7141 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7201 ccgcaagccc ggtgcctaac tgtttcgcct cggcgtccgg actagaggta cctttaagac
     7261 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7321 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     7381 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7441 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7501 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7561 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7621 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7681 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7741 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7861 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7921 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7981 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8041 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8101 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8161 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8221 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8281 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8341 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8401 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8461 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8521 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8581 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8641 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8701 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8761 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8821 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8881 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8941 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9781 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9841 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9901 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9961 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    10021 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
