LOCUS       sequence-464637-        9425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BFP-tagged vector for Turbo ID experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Nikolatou K, Sandilands E, Roman-Fernandez A, Cumming EM, Freckmann 
            E, Lilla S, Buetow L, McGarry L, Neilson M, Shaw R, Strachan D, 
            Miller C, Huang DT, McNeish IA, Norman JC, Zanivan S, Bryant DM
  TITLE     PTEN deficiency exposes a requirement for an ARF GTPase module for 
            integrin-dependent invasion in ovarian cancer.
  JOURNAL   EMBO J. 2023 Sep 18;42(18):e113987. doi: 10.15252/embj.2023113987. 
            Epub 2023 Aug 14.
  PUBMED    37577760
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-09-18"; volume: "42(18):e113987. doi"; pages: " 
            10.15252/embj.2023113987. Epub 2023 Aug 14"
COMMENT     Sequence Label: Start Stuffer TurboID V5 T2A BFP
FEATURES             Location/Qualifiers
     source          1..9425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             643..1599
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1606..1647
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1654..1707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1708..2409
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2432..3020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2485..2505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2903..2914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3092..3325
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3403..3524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3440..3459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3494..3513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3564..3699
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3719..3738)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3720..3738)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3748..3765)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3748..3764)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3757..3779)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3906..4361
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3993..4012)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4203..4224
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4387..4491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4492..4560,4561..5352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5523..6111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6012..6031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6265..6282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6399..6420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6435..6440,6441..6458,6459..6465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6473..6489
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6478..6500
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6497..6513
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6497..6513
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6532..6552
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6534..6552
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6580..6806
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6807..6987
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7034..7159
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7652..7885
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8070..8114
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8289..8789
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8708..8726
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8801..9196
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9256..9373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 gttaacgcca ccatggccgg atccggatca ggaagcatcg ataaagacaa tactgtgcct
      661 ctgaagctga tcgctctcct ggctaatggc gagttccata gtggcgaaca gctgggagaa
      721 accctgggca tgtccagggc cgctatcaac aagcacattc agactctgcg cgactggggc
      781 gtggacgtgt tcaccgtgcc cggaaagggc tactctctgc ccgagcctat cccgctgctg
      841 aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc
      901 gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc
      961 attgcagaat atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt
     1021 ggagctaacc tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc
     1081 ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag
     1141 gtgcgagtca aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg
     1201 gtggagctgg ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac
     1261 gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa
     1321 gcagggatta acctggacag gaatactctg gccgctacgc tgatccgaga gctgcgggca
     1381 gccctggaac tgttcgagca ggaaggcctg gctccatatc tgccacggtg ggagaagctg
     1441 gataacttca tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt
     1501 agccggggga ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc
     1561 tggatgggcg gagaaatcag tctgcggtct gccgaaaagc gtacgggcaa gcccatcccc
     1621 aaccccctgc tgggcctgga cagcaccacg cgtgagggca gaggaagtct tctaacatgc
     1681 ggtgacgtgg aggagaatcc cggccctatg agcgagctga ttaaggagaa catgcacatg
     1741 aagctgtaca tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa
     1801 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
     1861 cccttcgcct tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac
     1921 cacacccagg gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag
     1981 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
     2041 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct
     2101 gtgatgcaga agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac
     2161 ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc
     2221 gcaaacatca agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc
     2281 gtctactatg tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc
     2341 gagcagcacg aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag
     2401 cttaattaaa ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     2461 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2521 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2581 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2641 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2701 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2761 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2821 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2881 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2941 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3001 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     3061 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     3121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3301 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     3361 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3541 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3601 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3661 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     3721 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3781 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3841 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3901 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3961 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4021 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4081 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4141 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4201 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4261 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4321 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4441 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4501 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4561 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4621 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4681 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4741 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4801 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4861 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4921 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4981 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5041 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5101 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5161 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5221 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5281 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5341 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5401 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5461 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5521 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5581 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5641 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5701 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5761 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5821 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5881 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5941 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6001 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6061 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6121 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6181 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6241 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6301 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6361 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6421 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6481 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6541 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6601 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6661 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6721 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6781 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6841 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6901 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6961 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     7021 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     7081 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     7141 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     7201 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     7261 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7321 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7381 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7441 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7501 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7561 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7621 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7681 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7741 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7801 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7861 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7921 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7981 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     8041 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     8101 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     8161 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     8221 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     8281 ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     8341 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     8401 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     8461 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     8521 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     8581 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     8641 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     8701 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     8761 ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca
     8821 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     8881 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     8941 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     9001 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     9061 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     9121 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     9181 ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc
     9241 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9301 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9361 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     9421 ggggg
//
