LOCUS       sequence-464641-        3019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_KanR-NeoR-nptII_PartType2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK001 [Marker_KanR-NeoR-nptII_PartType2]
FEATURES             Location/Qualifiers
     source          1..3019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             975..1769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1029..1048)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1639..1658
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     promoter        1997..2088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2089..2157,2158..2949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2953..2992
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctcaata gcttcacgct gccgcaagca ctcagggcgc aagggctgct
      661 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
      721 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
      781 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
      841 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
      901 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
      961 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1021 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     1081 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     1141 ccctgaatga actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     1201 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     1261 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     1321 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     1381 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     1441 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     1501 cgcggatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     1561 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     1621 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     1681 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     1741 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     1801 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     1861 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1921 gctggagttc ttcgcccacc cccccttgag accagagtag cccgcctaat gagcgggctt
     1981 ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     2041 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag
     2101 cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat
     2161 ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat
     2221 attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt
     2281 ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg
     2341 ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc
     2401 ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg
     2461 attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa
     2521 gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa
     2581 ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg
     2641 gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa
     2701 ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg
     2761 gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt
     2821 gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc
     2881 caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa
     2941 cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt
     3001 gtcagatcaa aggatcttc
//
