LOCUS       sequence-464647-        3811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_TetR-tetAR_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK009 [Marker_TetR-tetAR_PartType4]
FEATURES             Location/Qualifiers
     source          1..3811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(620..1270)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             1376..2575
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     promoter        2789..2880
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2881..2949,2950..3741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3745..3784
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggct caatcgtcac cctttctcgg tccttcaacg ttcctgacaa
      661 cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt
      721 ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct
      781 gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg
      841 ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac
      901 ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc
      961 gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc
     1021 gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat
     1081 gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt
     1141 gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac
     1201 cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca
     1261 actttgtcat gcttgacact ttatcactga taaacataat atgtccacca acttatcagt
     1321 gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagacgtgaa
     1381 acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg tcggcatcgg
     1441 cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga acgacgtcac
     1501 cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct gcgcacctgt
     1561 gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct cgctggccgg
     1621 cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc tctatatcgg
     1681 gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt atattgccga
     1741 tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct gtttcgggtt
     1801 cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc cccacgctcc
     1861 gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt tccttttgcc
     1921 ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc cgctcgcttc
     1981 gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct tcttcatcat
     2041 gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg atcgctttca
     2101 ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc attcactcgc
     2161 ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg cactcatgct
     2221 cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac ggggatggat
     2281 ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg cgctgcaagc
     2341 aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct cactggcggc
     2401 gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct atgcggcttc
     2461 tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact tgctctgcct
     2521 gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc gctgatcgtg
     2581 gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat acgcgcccta
     2641 ggagtgcggt tggaacgttg gcccagccag atactcccga tcacgagcag gacgccgatg
     2701 atttgaagcg cactcagcgt ctgatcttaa ttaaaacgtg agaccagagt agcccgccta
     2761 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
     2821 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2881 atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg
     2941 gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg
     3001 cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg
     3061 gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc
     3121 cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg
     3181 gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg
     3241 tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt
     3301 ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg
     3361 gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg
     3421 ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca
     3481 agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt
     3541 agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc
     3601 cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat
     3661 accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg
     3721 agcctgatta aacattggta aaccgataca attaaaggct ccttttggag cctttttttt
     3781 tggacgaccc ttgtcagatc aaaggatctt c
//
