LOCUS       sequence-464648-        2665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_GenR-aacC1_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK010 [Marker_GenR-aacC1_PartType4]
FEATURES             Location/Qualifiers
     source          1..2665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(762..767,768..784,785..790)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             979..1512
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        1643..1734
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1735..1803,1804..2595)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2599..2638
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggca attcgaattc ctgccgacat ggaagccatc acaaacggca
      661 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      721 atggacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg
      781 ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      841 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      901 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      961 tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca gggcagtcgc
     1021 cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag gctcggccct
     1081 gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt cggagacgta
     1141 gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct ccgtagtaag
     1201 acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct cgcggcttac
     1261 gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct cgcagtctcc
     1321 ggagagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa gcatgaggcc
     1381 aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga tcccgcagtg
     1441 gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat cgacccaagt
     1501 accgccacct aacaattcgt tcaagccgag atcggcttcc cggccgcgga gttgttcggt
     1561 aaattgtcac aacgccgcca ggtggattaa cgtgagacca gagtagcccg cctaatgagc
     1621 gggctttttt ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1681 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagc
     1741 attcagcatt ttcgtgtggc gctgattccg ttttttgcgg cgttttgcct gccggtgttt
     1801 gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg
     1861 ggctatattg aactggatct gaacagcggc aaaattctgg aatcttttcg tccggaagaa
     1921 cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg
     1981 gatgcgggcc aggaacaact gggccgtcgt attcattata gccagaacga tctggtggaa
     2041 tatagcccgg tgaccgaaaa acatctgacc gatggcatga ccgtgcgtga actgtgcagc
     2101 gcggcgatta ccatgagcga taacaccgcg gcgaacctgc tgctgacgac cattggcggt
     2161 ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc atgtgacccg tctggatcgt
     2221 tgggaaccgg aactgaacga agcgattccg aacgatgaac gtgataccac catgccggca
     2281 gcaatggcga ccaccctgcg taaactgctg acgggtgagc tgctgaccct ggcaagccgc
     2341 cagcaactga ttgattggat ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg
     2401 ctgccggctg gctggtttat tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc
     2461 attattgcgg cgctgggccc ggatggtaaa ccgagccgta ttgtggtgat ttataccacc
     2521 ggcagccagg cgacgatgga tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg
     2581 attaaacatt ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg
     2641 acccttgtca gatcaaagga tcttc
//
