LOCUS       sequence-464649-        2489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Marker_CamR-cat_PartType4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK013 [Marker_CamR-cat_PartType4]
FEATURES             Location/Qualifiers
     source          1..2489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        636..738
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             739..1398
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(805..824)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        1467..1558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1559..1627,1628..2419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2423..2462
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttggcg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
      661 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      721 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      781 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      841 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
      901 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt
      961 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     1021 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     1081 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     1141 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     1201 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     1261 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     1321 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     1381 tggcagggcg gggcgtaatt tttttaaggc agaacgtgag accagagtag cccgcctaat
     1441 gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta
     1501 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1561 gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt
     1621 gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg
     1681 cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga
     1741 agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg
     1801 tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt
     1861 ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg
     1921 cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg
     1981 cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga
     2041 tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc
     2101 ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag
     2161 ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag
     2221 cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg
     2281 tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac
     2341 caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag
     2401 cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
     2461 gacgaccctt gtcagatcaa aggatcttc
//
