LOCUS       sequence-464654-        2428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_p15A_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK020 [Origin_p15A_PartType1]
FEATURES             Location/Qualifiers
     source          1..2428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      656..1201
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1318..1335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1406..1497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1498..1566,1567..2358)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2362..2401
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccgac tagaaatatt ttatctgatt aataagatga tcttcttgag
      661 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
      721 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
      781 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
      841 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
      901 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
      961 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     1021 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1081 gcgcacgagg gagccgccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1141 ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa
     1201 aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg
     1261 aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc
     1321 gagtcagtga gcgaggaagc ggaatatatc ccaattgaga ccagagtagc ccgcctaatg
     1381 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat
     1441 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1501 agcattcagc attttcgtgt ggcgctgatt ccgttttttg cggcgttttg cctgccggtg
     1561 tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc
     1621 gtgggctata ttgaactgga tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa
     1681 gaacgttttc cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt
     1741 gtggatgcgg gccaggaaca actgggccgt cgtattcatt atagccagaa cgatctggtg
     1801 gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg tgaactgtgc
     1861 agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac gaccattggc
     1921 ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac ccgtctggat
     1981 cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac caccatgccg
     2041 gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac cctggcaagc
     2101 cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct gctgcgtagc
     2161 gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg tggcagccgt
     2221 ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt gatttatacc
     2281 accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc
     2341 ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg
     2401 acgacccttg tcagatcaaa ggatcttc
//
