LOCUS       sequence-464655-        3920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_pSC101hs_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK023 [Origin_pSC101hs_PartType1]
FEATURES             Location/Qualifiers
     source          1..3920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      1099..1321
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1369..2319
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     promoter        2898..2989
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2990..3058,3059..3850)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3854..3893
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccgag acagtaagac gggtaagcct gttgatgata ccgctgcctt
      661 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
      721 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
      781 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
      841 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg
      901 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
      961 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
     1021 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc
     1081 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt
     1141 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa
     1201 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag
     1261 cagaatttac agatacccag atctcccggg aaaaggacta gtaattatca ttgactagcc
     1321 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta
     1381 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc
     1441 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa
     1501 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa
     1561 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc
     1621 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca
     1681 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa
     1741 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg
     1801 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag
     1861 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa
     1921 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg
     1981 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta
     2041 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac
     2101 aaaataccaa caaccattac atcagattcc tacctacata acggactaag aaaaacacta
     2161 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac
     2221 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc
     2281 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc
     2341 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc
     2401 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga
     2461 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac
     2521 agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga
     2581 acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct
     2641 gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc
     2701 tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg
     2761 gctgaagccg gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg
     2821 ggcagcaaaa cccgtaccct aggcaattga gaccagagta gcccgcctaa tgagcgggct
     2881 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2941 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca
     3001 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca
     3061 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta
     3121 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt
     3181 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc
     3241 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag
     3301 cccggtgacc gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc
     3361 gattaccatg agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa
     3421 agaactgacc gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga
     3481 accggaactg aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat
     3541 ggcgaccacc ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca
     3601 actgattgat tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc
     3661 ggctggctgg tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat
     3721 tgcggcgctg ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag
     3781 ccaggcgacg atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa
     3841 acattggtaa accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct
     3901 tgtcagatca aaggatcttc
//
