LOCUS       sequence-464656-        5367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_RSF1010_PartType1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK024 [Origin_RSF1010_PartType1]
FEATURES             Location/Qualifiers
     source          1..5367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(620..1471)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1458..2297)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2808..3779)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     rep_origin      3886..4280
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     promoter        4345..4436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4437..4505,4506..5297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5301..5340
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccgat cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg
      661 ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc
      721 gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca tagccgcaca
      781 aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac agccgctggt
      841 gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg atgcgcacat
      901 gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc
      961 cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc ttgcggccat
     1021 tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg
     1081 ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag ctacctttga
     1141 ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc cggagctgcc
     1201 gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca gccatggcca
     1261 ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg ctgaactcga
     1321 tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc
     1381 gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgc cggacgtggc
     1441 tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt gcgctgcctc
     1501 tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg atcagcgaac
     1561 ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg ctggtcgtcg
     1621 tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg
     1681 atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc
     1741 atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt atcggcggcg
     1801 atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc ttcctcgatg
     1861 tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc gcgcttgagg
     1921 ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg ctggatcagc
     1981 aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg
     2041 cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc
     2101 agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag ttgcagggcc
     2161 agcatggatt taccggcacc accgggcgac accagcgccc cgaccgtacc ggccaccatg
     2221 ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga acgcctccag aatattgata
     2281 ggcttatggg tagccattga ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg
     2341 ggtcactacc cccgccctgc gccgctctga gttcttccag gcactcgcgc agcgcctcgt
     2401 attcgtcgtc ggtcagccag aacttgcgct gacgcatccc tttggccttc atgcgctcgg
     2461 catatcgcgc ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct
     2521 tttggtcttt catatcagtc accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg
     2581 aacaaggaca aggtgcagcc gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg
     2641 ccagcctcgg ccttgtttga cgtataacca aagccaccgg gcaaccaata gcccttgtca
     2701 cttttgatca ggtagaccga ccctgaagcg cttttttcgt attccataaa acccccttct
     2761 gtgcgtgagt actcatagta taacaggcgt gagtaccaac gcaagcacta catgctgaaa
     2821 tctggcccgc ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc cagctcggcc
     2881 cgcgcaagct ggacgctggg cagacccatg accttgctga cggtgcgctc gatgtaatcc
     2941 gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg cctcggccat ggccttgccg
     3001 atttcctcgg cactgcggcc ccggctggcc agcttctgcg cggcgataaa gtcgcacttg
     3061 ctgaggtcat caccgaagcg cttgaccagc ccggccatct cgctgcggta ctcgtccagc
     3121 gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt cgaggctggc cagcctgcgg
     3181 gccttctcct gctgccgctg ggcctgctcg atctgctggc cagcctgctg caccagcgcc
     3241 gggccagcgg tggcggtctt gcccttggat tcacgcagca gcacccacgg ctgataaccg
     3301 gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg
     3361 ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc
     3421 cgaagttcac cgcctgcggc gtcggccacc ttgacccatg cctgatagtt cttcgggctg
     3481 gtttccacta ccagggcagg ctcccggccc tcggctttca tgtcatccag gtcaaactcg
     3541 ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct cggcgggcct gatatacacg
     3601 tcattgccct gggcattcat ccgcttgagc catggcgtgt tctggagcac ttcggcggct
     3661 gaccattccc ggttcatcat ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc
     3721 tcacagccca tggccttgag ctgtcggcct atggcctgca aagtcctgtc gttcttcatc
     3781 gggccaccaa gcgattccca cacattatac gagccggaag cataaagtgt agctagatcc
     3841 gaaggatgag ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc
     3901 cccacccttc gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc
     3961 tgcggggttt ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg
     4021 tggggcggtg tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat
     4081 attgggcaag ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc
     4141 ttagataatc atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg
     4201 caggtttggg ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg
     4261 acagttattg caggggttcg tgacagttag caattgagac cagagtagcc cgcctaatga
     4321 gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc
     4381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4441 gcattcagca ttttcgtgtg gcgctgattc cgttttttgc ggcgttttgc ctgccggtgt
     4501 ttgcgcatcc ggaaaccctg gtgaaagtga aagatgcgga agatcaactg ggtgcgcgcg
     4561 tgggctatat tgaactggat ctgaacagcg gcaaaattct ggaatctttt cgtccggaag
     4621 aacgttttcc gatgatgagc acctttaaag tgctgctgtg cggtgcggtt ctgagccgtg
     4681 tggatgcggg ccaggaacaa ctgggccgtc gtattcatta tagccagaac gatctggtgg
     4741 aatatagccc ggtgaccgaa aaacatctga ccgatggcat gaccgtgcgt gaactgtgca
     4801 gcgcggcgat taccatgagc gataacaccg cggcgaacct gctgctgacg accattggcg
     4861 gtccgaaaga actgaccgcg tttctgcata acatgggcga tcatgtgacc cgtctggatc
     4921 gttgggaacc ggaactgaac gaagcgattc cgaacgatga acgtgatacc accatgccgg
     4981 cagcaatggc gaccaccctg cgtaaactgc tgacgggtga gctgctgacc ctggcaagcc
     5041 gccagcaact gattgattgg atggaagcgg ataaagtggc gggtccgctg ctgcgtagcg
     5101 cgctgccggc tggctggttt attgcggata aaagcggtgc gggcgaacgt ggcagccgtg
     5161 gcattattgc ggcgctgggc ccggatggta aaccgagccg tattgtggtg atttatacca
     5221 ccggcagcca ggcgacgatg gatgaacgta accgtcagat tgcggaaatt ggcgcgagcc
     5281 tgattaaaca ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga
     5341 cgacccttgt cagatcaaag gatcttc
//
