LOCUS       sequence-464658-        6388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-pMlb_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK026 [Origin_repABC-pMlb_PartType3]
FEATURES             Location/Qualifiers
     source          1..6388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5366..5457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5458..5526,5527..6318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6322..6361
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctg gcgttcgtca ctcgtttggc catgcggaat ctccagccgg
      661 aacaccccct cgtcgcccgg ctggcggtcc gcgcgggatt ggcttgcgat ttcactttgt
      721 cagcaagcgg tgctgtttgc gcatcgtgac gggttggtac cgctggtcta ttgccggccc
      781 ctaccccgat agatttcatc cgcattgaca cgacgatgtg ttgaagccgg gaaatgtgac
      841 gagccgtctt caggagtctt tggcctcggt ctggcgatgc tggccgtcag catgcgtctc
      901 aacatagcgg tcttggcaac accaccgcgt acctacgatc tgaaactttg ctccgccgaa
      961 tggtcgcgtc tggtgcgcgc aaatccatgt tttcaatatc gttctgcgat ttttttactg
     1021 cggtaaattt ctggatgcgg tcagtgcgat tggccgcgct ctggccagcg ttaacctttt
     1081 gttaacccaa aacttcttgc cggtaagtcc gaatcggagc ttaatggcgc ttgttgtggg
     1141 cattgcttac aaaaagcgcc aattcaggtt tcgacgagaa tgaatgtctc catccggctg
     1201 ccacgtttcg agttcgatga caaaatcctc gaacagggcg ccgagatttc ccggcggttg
     1261 aaccagcttc ggatggagaa atttccgccc aatgcgacga agacgctacg cacattctcg
     1321 atggcggagg tcgctcacta cctcggggtt tcgcagaaca atttgaagcg tctccatctc
     1381 gaaggcaagg ggccgttgcc tgaccaaagt ccttccggcc gtcgctccta cacggctaca
     1441 cagatgctcg agctcaggca gttcctggac cagcatggca ggactgaagt caagaagtac
     1501 gttccgcacc gcaagcttgg cgataagctc caggtggttg cggtggtcaa tttcaaaggc
     1561 ggcagcggca agacgaccac ggcagcccac ctcgcccagc acctggcgct gactggtcat
     1621 cgggttcttg cgatcgatct tgatccgcaa gcttcgctgt ctgctctgca cggcttccag
     1681 cccgagctcg atcgcaacct ctcgctctat gaagcgatca ggtatgacga cgaacgcaga
     1741 tcgattgcgg atgtgatcat accgacaaat ttccccggcc tcgacatcat tcccgccaat
     1801 ctcgagctgc aggaatacga gtatgacacg ccgctcgcca tgcaagacaa aacctccagt
     1861 gccggccgcc agttcttcac gcgcatggcc aaagcgttgt cggaggttga cgatcggtac
     1921 gatgtcgtcg ttgtcgattg cccgccccag cttggctatc tgactttgac ggcaatgtcg
     1981 gcggcgacat cggttctcat tacggtccat ccgcagatgc tcgacctgat gtcgatgagc
     2041 cagtttcttc tgatgcttgg cggcatattg aagaccatca aggccgccgg cgccgcgatc
     2101 gaacttgact ggtttcgcta cctgatcacg cgctacgaac cgaccgatat tccgcaggcg
     2161 cagatggtgg gcttcatgca gtccatgctt gccggccaga tcctggaaaa cccgatgttg
     2221 aagtcgacgg cgatttccga tgcaggcctc accaagcaaa cactttacga ggtggagaaa
     2281 tcggccttca cccgctcgac atatgaccgc gctttggaat cgctcgacgc ggtgaacgcc
     2341 gagatcgtca cactcatcca tcgtgcgtgg ggtcggtcat gacggcgaac tttacggcag
     2401 taaaaaccgg ctattttgct cgtggaatca gatcgctagc cgctcacgga ggcggttgaa
     2461 tggcccgtaa ggatattttc ggcagtgtga tgggacccgt cgctgcggtg gccgataaca
     2521 aggatgtatc ggggtatgcc gttcgtggtg cctcccgctc gatcatgcaa tccttcgagg
     2581 agctctccaa gagttccgtc gtcgatctcg atcctgccct ggttgacacc tcgttcgtct
     2641 cggaccgcct ggacgaagac gatcaggcgt atcaggagtt gctgtcggca attcgcgaga
     2701 gaggtcaaga ttctccgatt ctggtcagac cgcatccgga ggtttcagga cgcttccaga
     2761 cagtattcgg ccatcgccgc gtcaaggttg cccgccagct gggaaggccg gtccgcgccg
     2821 tcgtcaagaa gatgggcgac gaggaccatg tcatcgccca gggccaggag aatgccgcca
     2881 gggccaatct gtccttcatc gagaggaccc tgtttgccga tcgcattctc aagcgcggcc
     2941 acacctcgga cacggtgaag tccgctttgg cgctcgatga cacgaccttt tcgaagatgc
     3001 gctccgtgac cggcaatatc cccgagccag tcattgtggc tgttggctcg gccaaggccg
     3061 tcggacggga caggtggtgg caactggcca agctcatgga gcatcctggg aacgcgggca
     3121 gggcggccga ctatgtgaag agcgctgatt tcgcggagcc aagcagtgac gcacgatttg
     3181 cgcttctttt cgactacctg aacgctccgg cgaaacgttc gccggcagcg ccgaagacac
     3241 gagaaaaggc ctgggcgccg caagacaggt cggtgcgggc caagatcacc gacaccggaa
     3301 aagtgttcac gcttgccctg aaagcaaggg aagcctcgcc gttcggtgcg ttcatcaccg
     3361 aacggctgga cgagcttttc gaagcgttcc ggcaatcgga aatgtctaag agaacaggag
     3421 actgaaaccg caaaagaaaa aggcccccga acgtcgccgt cgcggaagcc cttctcgtct
     3481 gtagcaagct gagaatcgca cttccccgaa tcactgtcaa gagtcgtggc gccatttcgg
     3541 cgagcaggtt tcttttgcct tcgtgaaagg tgagggaaat ggagagtggg tttgcgacga
     3601 cgccttttgg gcggcggccg atgacgcttg ccttggtgaa ggccaatgcc gcgtcgcggc
     3661 agattccgaa aggggccgcc gttgagaaat ggcaggtcta ccggtggcta tgcgagggca
     3721 agtcgatcgt cggcgttggc gatcgtgccc tggcggtgct gagcgggctg ttgacgtttt
     3781 atcctgacga ccagatcagc gaggaaaacg gcttgatggt cttcccgtcg aacgcacagc
     3841 tgtctttgcg ggcgcacggc atggccgatt cgacgctgag aaggaacctg gccgagctgg
     3901 tgagttgcgg cctgatcatt cgccgcgata gtccgaacgg caaaagattc gctcgtaagg
     3961 ggcagggtgg tgctgtcatg gaggtcttcg ggttctcgtt ggcgcctctg ctggcacggg
     4021 cggcggagtt tcagcgcgcc gccgagcagg tccgcgccga gtgccaggcg ctgaaattga
     4081 tgcgggagcg catcacgctg catcgcaggg acattcagaa gctggtcgag gcggcgatcg
     4141 aggaaggggt tccgggcgac tggggcggcg tctggaggcg gtttcggtcc atcgtggatg
     4201 caattccgcg ccgggcgggg ctgtcacagc ttgaagggat cgttgccgag ctggtcgtgc
     4261 tccacgatga agtggatagg atgctggaaa gcttcacaag ttccacgaat cctagcggca
     4321 atgagtccca aaatgagcgg cagcaatctg attcaaacac agactccatt tttgtatttg
     4381 aaccggcttt cgaaaaaagc agggcggccg ttgagccaag accggcaccg gacgagcggt
     4441 tgaaaagcta tccgattgga ctggtgctga aggcctgccc cgagtttgcg ggctatgctg
     4501 tcaacgggat cgccagctgg cgcgatttga tgctcaccgc ggcccaggtg cgcggctacc
     4561 ttggggtttc accgtcggcc tatgaggagg cttgccatgt gatgggtcag gaagtcgccg
     4621 cgattgtgat tgcgtgtatt ctgcaacggg cacagcacat caattcggcc ggtggctatc
     4681 tgcgcgtgct gacggaaaag gcgagggcag gggagttctc ggtcggaccg atgctgatgg
     4741 ccgcgctcaa ggccaatggc ccggcaacga ggatgacggg atgattgagg tacaggttcg
     4801 tggggcaggt tgctcggcgc tctaggtgac ggcatgcggc cacctaccgg cggtgggctc
     4861 ccgggcttga aagctgttca ggaatgccat atcttttgca caagaaacat gactcctgat
     4921 atggtgaaca ctgtgggcca tgcactcaag gcagccgagc agaatgggcc gctcgtcttg
     4981 tcctatatgg atcggaacgg gaagattgcg atcgagcagg tcgcggacgg ttttggcatg
     5041 tccaagggtc agttggccca gaccgccggt cttgcgcgtg agacactcta tcgctcagag
     5101 cgcagcgctg cggcaaagac ccaggggcgt ctgcgcgaaa tgctcgagat catcagccgc
     5161 gtgagcgatt gggcgggcgg caaggaacag gcgatggcct ggtatcgggc tcagccgctt
     5221 ccggcatttg gcggacgcac tgccgaagcg ctggtgaagg atggcaaggc cgccgcggtc
     5281 cgtgattatc tagatcacat ggcagtcggt gtggctgaga ccagagtagc ccgcctaatg
     5341 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat
     5401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5461 agcattcagc attttcgtgt ggcgctgatt ccgttttttg cggcgttttg cctgccggtg
     5521 tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc
     5581 gtgggctata ttgaactgga tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa
     5641 gaacgttttc cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt
     5701 gtggatgcgg gccaggaaca actgggccgt cgtattcatt atagccagaa cgatctggtg
     5761 gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg tgaactgtgc
     5821 agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac gaccattggc
     5881 ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac ccgtctggat
     5941 cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac caccatgccg
     6001 gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac cctggcaagc
     6061 cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct gctgcgtagc
     6121 gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg tggcagccgt
     6181 ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt gatttatacc
     6241 accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc
     6301 ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg
     6361 acgacccttg tcagatcaaa ggatcttc
//
