LOCUS       sequence-464659-        6633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-pMla_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK027 [Origin_repABC-pMla_PartType3]
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5611..5702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5703..5771,5772..6563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6567..6606
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtcgccatca
      661 aaccaagcga gctggccgtg cgcgccagcg cccgaccgcg cggcgcaaga gcaacgatga
      721 ggtcatcgcc gatattttgc ccgccctcct tggcagcctt cgctgggaac cgtctcctct
      781 cggactcggc caccgtgccg ttttgctggc tgctctcgca tctggtggtc gccgtcgcag
      841 cgaaactgct actctccccg cgtcgaacag ggtcgaggaa gagtcggtgc cggcccgatc
      901 ccaaggcgca ccccgacggc gaaaggctgc cctgcctgtc gatccgctgg catcaagacg
      961 acacaggccg gcagcgacgc tttcgtgctg ccagtcgccc ggcaggtgcc tttgccgcag
     1021 tcgtggttgg atcgggccgg gtagtggtcc agcacaggcg acagcgcact ggagtaaacg
     1081 tatggcggaa ggccgggagg gtgggttcgc gctgagagtg ccgcggtgct cgagcgactt
     1141 cagtcctcct ttttcttcac gtgaatccag aacggagcag ttggccgcgg ccaactttgc
     1201 aggttgaccg cgacagaggc actattggca ccggtgcgat tcgcacgaaa gaagagttgg
     1261 ccgcggccaa cttttgacgg ccgaccaccg gaaaccgttg aatcataagg gttcttggca
     1321 tggttaccga atcgtgccat gttcgatacg gttttttagc aattcgccgc taaaaagcgc
     1381 atcgaaaggg gaaatcgatg ttgcagactc aaaacgtatc gacggccatt tcttccgacg
     1441 cgcggattgc tcatcatgcg cgccagctgt ctatgcagct gcagttactg cgagagcgcc
     1501 tgtttccgcc gtcctcccag aagatgttga agacgttcac ctccggcgag gccgctcagc
     1561 tcgtcggggt atcggacggc tacctacggc agctttcaat agatggcaaa ggccctttgc
     1621 ccgaggtatc ccagacggga cgacgttcgt actcgctcgc ccaaattcat gatttgcggc
     1681 agtacatggc ttctatcaag ccgaaggatg cactcacgta ccttccgtgg cggcagagag
     1741 gcgaaaagct ccagacgatt gccgttgcca acttcaaggg cggcagtgca aagacaacga
     1801 catccatcta cctcgcacag tatcttgcct tgcagggata tcgtgttctc gcggttgatc
     1861 tcgaccccca ggcttcgttg tcgtcgatgc tcggtgttca gccggagttc gatctagcgg
     1921 aaggcgatac gctctatggt gcgatccgtt acgacgagaa acggcggccg ctgaaggaaa
     1981 taatcagaaa aacgtacttc gctggcctcg acctcgttcc cgggaacttg gaactgatgg
     2041 agttcgagca cgaaaccccg actgcactga tggagcagcg gaaagcaagc aacagcgaaa
     2101 tcttctttcg gcgcgtcggc atggccttgg ccgaagtcga agccaattat gacgttgtgg
     2161 tggtcgactg cccgccgcag ctcggatacc tgacgcttgg tgcggtttgc gcagcgacat
     2221 cgcttctgat caccgtccat cctcagatgg tcgacgtggc gtcaatgtcg cagtttctgc
     2281 tgatgacttc cgaccttctg tcggtcgtcc ggaaagcagg cggcgatctg aaccacgatt
     2341 tcatccggta tgtcataacg cgccatgagc cgcacgacgg tccccaggca cagatcgttg
     2401 ccttgctgag aagtcttttc ggggacgagg ttttggcggc gacagtgctg aaatcgacgg
     2461 cgatcgccga tgccgggctt acgaaacaga cactctatga aatcgaacga ggacaagtcc
     2521 gccgctcgac attcgatagg gcgatcgaat ccctggatgc agtcaacggg gaaatcctcg
     2581 gcggcatcga aaaggcatgg gagcggtcat gagcaggcga gacgttctga acggaatatt
     2641 tgccgatacg acccgagagt tggccgcggc caacttttcc aaagaggcgg atggcggtcg
     2701 tgttctggcg ggtcccgtaa ggacgatggg acttgccctc gaccgcatgg accaggaagc
     2761 cagagaactt cgggaagcct tgaagtccgg cgagagggtt gtcgaactcg atcccgaact
     2821 cattgacggc tcattcgtgc gtgatcgcct cgacggcgag gtacccgcta cggacgatat
     2881 tgtgaggtcg atcgaggaaa acggccagga agtgcccatc ctcgtcaggc agcaccccga
     2941 tctcgaagga cgatatcagg ttgcctatgg ccataggagg cttcgtgctg tccggctcct
     3001 cgggcggaag gtaagggcgg ttgtcaggcg attatcggac aatgaactcg tcgtcgcaca
     3061 aggcatcgaa aacactgcga gaaaggatct ctcttacatc gagagagcgg tctttgcctt
     3121 aagcctcgag acacacggct tcggccgcga tctgatcatg caggcgttaa gcactgagaa
     3181 gacggaactg tcgaaattgt tgtccgtcgc caagggcatt ccaggcagcc tagtcaaagc
     3241 tatcggtgcc gcaccctcta tgggccgtcg caggtggatg gatattgccg aaaagatcac
     3301 tgatcccaaa gcgatggaag tcgctagaaa tgccatccac aggcctgaat tcgaggcctt
     3361 aggctcggac gaacgcttca tgatggtttt ggctgaggtt tcgaagaagc cgccgcgaga
     3421 gcagaccatc agcgagtgga aaccgaacgg tggcaaggtt gcggcaaaga tcaaggacac
     3481 cgggagggcc tatacgctct ctctcaagac tgccgaagcc gatgaaggct tcggcgcata
     3541 tctgacaaaa cggctggatg atctctatgc cgactggcag gcaaacagga aatcgaagca
     3601 ggagtgaacc gcaaaagaaa aaggccccca aacgacctta gtcgtggaag cctctctcat
     3661 gttgtcgcaa actgagaatc gcatttccac gaatcactgt caagagtcta ggcgtcgatt
     3721 cggcgagcag atttcttttg cctaatcgag ggtgaaagaa aatggagacg ggtattgcaa
     3781 cgacgccctt tgggcggcgg ccgatgtcgc ttgccatgct ggcagcgcaa aacgagtcac
     3841 gggagatccc caagggcaag gtcatcgaca agtggcagat ctatcgcaac ctctgcgagg
     3901 gcaagagcat cgtcggcatc ggcgatcgcg ccctggccgt cctgaatgcg ttgctgtcat
     3961 tctatcctga cagcgagctg agtgaggaaa acggcctcat cgtcttcccc tcgaacgcac
     4021 agctgtccct cagggcgcac ggcatgccgg attccacctt gcggaggaac ctggcggaac
     4081 tggtggattg cggtctagtc atccgtcggg acagcccgaa cggcaagcgt tatgcacgga
     4141 agggcagggg tggggagatc gaggaagcat ttggcttttc cctggcgccg ctgctggccc
     4201 gtgctcacga gttcgaggcg gcggccgagc gtgttcgcgc cgacaacagg gcgctcaggc
     4261 ttatgaggga acgcatcacg ctacaccgcc gggatatcca gaagttggtc gaggcggccg
     4321 ttgaggaaga cgtgccgggc gactggggag gcctgtggag gcgtttccgc gccgttgtgg
     4381 aggcaattcc gcgccgagca tgcattgccg agctcgaacc gatcgtggcc gatctggccg
     4441 cgctgcgtga tgaggtggat aagctgctgg aaatccatat gaaatccacg catccgagcg
     4501 gcaatgactc tcaaaacgag cggcagcaat ctgattcaaa taccgactct atttttgaat
     4561 ttgaacctgc tttagagaaa agcggggcga cggccgagcc aaggacgagg accgcagagg
     4621 cgccgaaaac atacccgctg gggatggtgc tgaaggcctg ccccgaaatt gcggattatg
     4681 ccgtcgacgg gatcggcaat tggcgcgatc tcatgataac ggctgcccag gtgcggggat
     4741 atctgggcgt ttcgccgtca gcgtatgagg aggcttgcca tgtgatgggc caggagatcg
     4801 cggcgatcgt gattgcctgc atcctgcaaa gggcgcagca catcaactcg gccggcggct
     4861 atttgcgggt gctgaccgag aaggcgaggg caggggagtt ttcggtcgga ccgatgctca
     4921 tggctgcttt gaaagctaat gggtcaccta ccaggatggc cgggtaaccg gctaatgtga
     4981 cgatggccgc cgtcgcggct tgttgatgac agtcagaaac tgtgcttctt cagatcccaa
     5041 cggcaggacc agccgtcccc ccggtttgag ttgcttcact aaagccggcg gcgtctgctc
     5101 agccgccgcc gtgaccagga tcttgtcgaa tggggcgtgc tcgggccagc cgcgagacac
     5161 gtcccagaca ccaatgccga cattggataa atcgagcccc ctgcaggaga gcctctgcac
     5221 tgcttgcgaa ttcctcgacg atttcgacgc tacagacttg cccggcgagc ttcgcgagaa
     5281 ctgcggtttg gtagcccagg ccggtgccga tctcgagtac cgcctcgtgc ggttgcggtg
     5341 caaggagatc ggtcatgaga gcaacgatga agggctgcga gacggttttg tcaaagccga
     5401 tccacagcgg catgtcctgg taggcgtaag gcacaaccga tgctggcacg aagcgatgcc
     5461 gcggcacccg ccgcatcgct gccatcgccc gctcatcgag cgccgccttg ccaagttctt
     5521 cgcactggat tgtatcaacg gaccagtgac agtttctggc tgagaccaga gtagcccgcc
     5581 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata
     5641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5701 gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc
     5761 cggtgtttgc gcatccggaa accctggtga aagtgaaaga tgcggaagat caactgggtg
     5821 cgcgcgtggg ctatattgaa ctggatctga acagcggcaa aattctggaa tcttttcgtc
     5881 cggaagaacg ttttccgatg atgagcacct ttaaagtgct gctgtgcggt gcggttctga
     5941 gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat tcattatagc cagaacgatc
     6001 tggtggaata tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac
     6061 tgtgcagcgc ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca
     6121 ttggcggtcc gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc
     6181 tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca
     6241 tgccggcagc aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg
     6301 caagccgcca gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc
     6361 gtagcgcgct gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca
     6421 gccgtggcat tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt
     6481 ataccaccgg cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg
     6541 cgagcctgat taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt
     6601 tttggacgac ccttgtcaga tcaaaggatc ttc
//
