LOCUS       sequence-464661-        6566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-p42f1_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK030 [Origin_repABC-p42f1_PartType3]
FEATURES             Location/Qualifiers
     source          1..6566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5544..5635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5636..5704,5705..6496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6500..6539
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtgatgacgg
      661 aaaatggctt tcggaccgag gacggcctgc tcctgccgag cgagcgtcac cccagggacg
      721 ccgtgactta cggcgttcgt cccgaacata tccgaatgga ccctgacggc atcgaggtga
      781 cgactgtcgt cgtcgagccg accggctcgg aaacccttgt catcgtccgg ctgggcaccc
      841 agacgctcac ctgcgtcttc cgcgagcgga tcagagccgc tcccggcgac gtgctgagga
      901 tcgcccctgt tcatgacacc gttcacctgt tcggcgagga cgaacagcgc atcacgacag
      961 gcgaagcgcc actaagttaa gcccgccgaa tgtcccctcc ccttcggccg ggcgccgccg
     1021 ggggcgcgtc cgccgcgccc ccggcccgaa gttcccggct cttccatgca catgtgaagg
     1081 acgccaagtg cttgaacgag caccgaaaag gttaatatgc gttaacctta aatttcttgg
     1141 cgggcatcgg agaatcgcgg ataataacgg ttataggggc ttttgatccc gaaaatcgtt
     1201 atccaagaga ttgctaccga tgaacgctaa ttcgaacgcc ttgaaggcta cgccgctgca
     1261 tttcgaggat cagattctcg agcacggcga tgtgatttcg aagaagctgc atctcttgag
     1321 cgtgcagcgc tttccgccga acgcaaagaa gacgctgcgc agcttctcgc tcgccgaggt
     1381 cgccaattat gtcggggcgt cgcagagcac gctgaagaaa ctgcatctcg aaggcaaagg
     1441 cccctgcccg cagacctcac cttccgggcg gcgctcctat agcgccgagc agatgctgga
     1501 gctcaggcgc tatctcgatg cgcatggtcg ctccgagagc cgcatgtatg tgccccatcg
     1561 ccgcggccat gagaagctgc aggtcatcgc cgtcgtcaat ttcaagggcg gctcgggcaa
     1621 gacgacgaca tcagcccatc tcgcccagca tctggcgctg accggtcatc gcgtgcttgc
     1681 catcgatctc gatccgcagg cgtccctctc ttccctgcac ggcttccagc cggaattcga
     1741 ccaggcctcc tcgctctatg aggcgatccg ctatgacggc aagaggaagc cgctctctga
     1801 gatcatccac aggacgaatt ttcccggcct cgatatcgtg ccggccaatc tcgacttgca
     1861 ggaatatgaa tacgacacgc cgctcgccat ggccgacaaa tcgagcaatg acggcaagac
     1921 cttcttcacc cgtatctcgc gcgcgctcgc tgaggtcgat gaccgctacg acgtcgtcgt
     1981 catcgattgc ccgccgcagc tcggctacct cacgctgacg gcgctgacgg cggcgaccag
     2041 cgttttgatc accatccatc cgcagatgct cgacgtcatg tcgatggggc agttcctgct
     2101 catgctcggc ggcatcctga agccgatccg tgccgccggc gccgaagtga acctctcctg
     2161 gtaccgctat ctcattaccc gctatgagcc gaccgacgtg ccgcaggcgc agatggtcgg
     2221 cttcatgtcg acgatgttcc acgaattcat gctgaagaac cagatggtca aatccaccgc
     2281 gatctcggat gccgggatca ccaagcagac gctttacgag gtcgagcgcg cctcgatgaa
     2341 ccggggaacc tatgatcgcg cgctggaggc catgggcgcc gtcaacgcgg agatcgccgg
     2401 ccttattcac caggcatggg ggcggtgagt ttgtcggctg acaaattcgc cgtttccgtg
     2461 aagcaaatca atggaatgac cgcaatcgga ggaagcgatg gcacgaaaga atctgctgga
     2521 agggctggcc gagatgccgg acgaaaacgc gggcgcgccg aactatccga tgcgcggggc
     2581 aggccggtcg ctcgtccgct cgctggatga gctcgccaag caggcggaaa aatttctgga
     2641 gggcgaggca gtggtcgacc tcgaccccga gcttgttgaa acctcgttcg tcaaggatcg
     2701 tttgtcggag gatgacgagg cgttccgcac gctggtcgag gcgatccgcg cacgcgggca
     2761 ggacacgccc atcctggtgc gtccccacgc caggatcgac ggacgctatc aggtggtctt
     2821 cggccatcgg cggctgcgcg ccgcccgcga gctcggccgc aatgtccgcg ccgtcgtcaa
     2881 ggcgatcgac gaccggaccc atgtcatcgc ccagggtcag gagaattcgg cgcgcgccga
     2941 cctcaccttt atcgagcggg cgctcttcgc caagcggctg gaggagctcg gctacgacag
     3001 ggaggtgatc tcgacggcgc tcgccgccaa tgcggcttcg gtttcgaaga tgatgtcggt
     3061 gatggagcgc ctgtcgcgtg aggtcgtcca ggcgatcggc tccgcacccg gggtcgggcg
     3121 cgagcgctgg gtggaactgt cgctgctcgc cggcaaggcc gccaatgcgg cgaatgtcgc
     3181 caaggtcctc gaaggcgaca actttcatca gttaccttcc gacaaacgtt tcgaagcgct
     3241 ctatgcggcg ctgaacaaaa gcgcgcggcc ggtcaagaaa accgtcgccc cgaggaaggt
     3301 gacatggcag ccgcttgata aggctgtcca ggcggaaatg aagaatgacg gcaaggcgtt
     3361 ctcgctctcg atgaaggcga ggaatgccgg ccgcttcggt gaatttctct ccggcaggct
     3421 ggatgcgctc tatgagcaat tcctagccga ggagagcaaa caaggagact gaaaccgcaa
     3481 aagaaaaagg cccccaaacg aacgagtcgt ggaagccctt ctcacatcct taagcaagac
     3541 agagaatcgc attttcacga atcccagtca agagtcctga agcgccctcg cgtcttttca
     3601 gacgtggcaa tgacgctgca gcgcttttcc tcctcgcatc gtttcggtga ccgaatttct
     3661 tttgcctgaa tagaggtgaa ggaaatggag agtgaatctg tgacgacgcc cttcgggcgg
     3721 cggccgatgt cgcttgccct gctgctgaaa cagcggcgcg gcgaagcgat tgcggccggg
     3781 acgacgcgca acaaatggaa attgttccga tcggtctgcg aggcgcgggc agggctcggc
     3841 gtgaccgacc gggcgctcac cgtgctcgat gcgctgctga gcttctatcc tgaggacgaa
     3901 ctctccagcg aaaaagggct ggtggtcttt ccctcgaatg cgcagctttc gctgcgagca
     3961 cgcggcatgg ccgccgcgac gctccgccgg catctggccg tgctcgtcga cgccggcctg
     4021 atctcccgca aggacagccc gaacggcaag cgttatgtcc gccgcggcag ggcaggcgtc
     4081 attgacgagg cctatggctt cagtctcgcc ccgcttctgg cccgcgcctc ggagatcgag
     4141 gcaatggccg ccgggctcgc cgctgaccga cagatgctgc gggcgatgaa ggagcggctg
     4201 acgatctgcc ggcgcgacat cgccaagctc atcgccgccg ccgtcgagga agccattccc
     4261 ggcgagtggg agcgaatttc ttcgctgttt cacgcgctgc tggaacgcat cccgaaggcg
     4321 gcaacacccg aaaatctgcg gccgcttcta aaggagctgg aaatgctccg cctcgccatc
     4381 atcaacacat tggaatcgcg agagaaaatt caaaatgcga gcaccaatga atcccagttt
     4441 gagcgccaca tacagagttc gaatcccgag tccctatctg aatctgaacc gggcctggaa
     4501 acggcgcagg aggcgaggct ggacgatcaa ccgaggtcag ggctgaaaga cgatgatcaa
     4561 ggcaggcgag gggatggcgg gcagggattg cgggctgttc ggacggatgc agggagattg
     4621 aaatccttcc agctcggcac ggtcctgaag gcttgccccg atatcgccga ttatggcccg
     4681 ggcggagcga tcggcagctg gcgggatctc atgacggcgg cggtcaccgt gcgcacgatg
     4741 ctgcgtgtcg gcccgccggc ctacgaggaa gcctgtgcgg tgatggggcc ggaaaatgcc
     4801 gccaccgtca tggcctgcat cctgcaaagg ggagggtgca tcacctccgc cggcggctat
     4861 ctcagggatc tcacgcgtcg cgccgaacgc ggcgaattcg cgcccggccc gatgctgacg
     4921 gcgctgttgc gggcgaagga tccgccaatc gccgaagtta gctgacggtg ctctttctgc
     4981 cgcgcagaag agcggcgcgg atctgttcgt tcttgctgag cacgggcgtg ggaaccagtg
     5041 ccggctcggg cgcctcgacc ttggcgctct gtttgaggct caggaagcgc ttctgcgcga
     5101 ccagccggtc ttcggccgac aagggttcga ccggttcgcc gtcgatgttg tggcgcatgg
     5161 aatcgggctg ggcgctggcg aaataatagc gccggcaatg gacaaaggca gcggtcgcgc
     5221 gccggagcgt cgttacaccg gcctcgggct tcaagagcgg acgcagctcg ttgaagaggc
     5281 cgacggcgaa gggaagaacg ggatcgccgg gcttggccgg aagcacgccg accggccgga
     5341 tcaacatgct gttgatcgcg ttggccttct ccacgtcgag ctcggtcgcc gcaatcggcc
     5401 cgcggctgat cttccagggt ttgtccatgc atcctccata aaggccgact gtgatgactg
     5461 gattgtatca acggaccagt gacagtttct ggctgagacc agagtagccc gcctaatgag
     5521 cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5581 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5641 cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt
     5701 tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt
     5761 gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga
     5821 acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt
     5881 ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga
     5941 atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag
     6001 cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg
     6061 tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg
     6121 ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc
     6181 agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg
     6241 ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc
     6301 gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg
     6361 cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac
     6421 cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct
     6481 gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     6541 gacccttgtc agatcaaagg atcttc
//
