LOCUS       sequence-464662-        6705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-p42f2_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK031 [Origin_repABC-p42f2_PartType3]
FEATURES             Location/Qualifiers
     source          1..6705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5683..5774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5775..5843,5844..6635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6639..6678
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtggttgcta
      661 atcgggacta agaaacaggc aggcggcttg tcggctgctc atagggcata gtgcgatgac
      721 catcggcatg tcaccgaaag acggcagtgg ctgacactat ttggacggaa gcgatcgcgc
      781 atcgccgcgg tgaaattgct cggctgccgt acgaaccgca gcctaagcat tctcgaaccg
      841 aacaaagggt tttgacgggt cgtgatgacc aacggcgaca acacctgaag gatgacgggc
      901 catggttacc ttgagcctgt ctgtgccgct gtgccgcaaa atcgcaatga atttcacatg
      961 tataatgaga atcgaggcct tgcctgtcgg catcttgcgc ggtgcgaatc accgccgttc
     1021 aatccgtcaa acctccggtt tccggagccc gttttgccgg ctctgatgcg cgttctggtt
     1081 aagcatttgt aattcggtca cgaatcgcgg catatagtaa ctgcgcaact gaagcgcttt
     1141 cgttcaaaat tgcgcagtta cgcaggaaag agatcatgca gccgagtctt gctcttcaag
     1201 atgatcaaga gcaccttccg tcgcttctgg caacggatgc gaaggagttg tcctatcaac
     1261 ttcagcagca tcaggccaaa atctttcctc cgctatcgca aaagacgatc aggacatttt
     1321 ctccggcaga ggccgcggcc ttcatcggca tcggcgaagg ttatctccgg caggtggcgg
     1381 ccgacggcca tggtccggat ccgctggcaa acggacgacg gctctatagc gcaaccgaca
     1441 tggatcggat ccgccgcgtc ctcgacgagc ggaacggaac gccgaaatat gtgccggccc
     1501 gcaggccggg cgaaaagctc cagatcgtct ccgtgatgaa tttcaagggt gggtcgggca
     1561 agaccacgac cgcggcccat tttgcgcagt tcatggcgct ccggggctac cgcgtgcttg
     1621 ccgtcgacct cgacccgcag gcctccttgt ccgcgctgtt cggccatcag ccggaattcg
     1681 acgtcggcga gggcgaaacg atctacggcg caatccgcta tgacgatccg cgcccgatcg
     1741 ccgatatcgt gcgcgccacc tatacgccga acctgcatct cattcccggc aatctggaat
     1801 tgatggaatt cgagcacgag acgccgaagg ccatggcatc gggcacggcg gagacgatgt
     1861 tctttgcccg tatcggcgag gtgctgaccg atatcgaaag cctctacgac atcgtcgtcg
     1921 tcgactgccc gccgcagctc ggcttcctga cgatgtcggc gctctgcgcg gcgacttcgg
     1981 tgttgatcac cgttcacccg cagatgctcg acgtcatgtc gatgtcgcag ttcctgacga
     2041 tgacgagcga actgatgtcg gtcgtcgaga gggccggcgg gcgcaccagc tatgactgga
     2101 tgcgctatct cgtgacccgg ttcgagccga atgacgggcc gcagagccag atgaccggct
     2161 tcatgcgggc gatcttcggc aatcgcatgc tccacaatgc catggtgaaa tcgacggccg
     2221 tttccgatgc cggcgtcacc aagcagacac tctacgaagt cgagcggtcg cagttcacgc
     2281 gcggaactta tgatcgggcg ctggagtcgc tgaacctcgt gaatggcgag atcgaagcgc
     2341 atattcgctc cacctgggga aggagataga aatggcgcgc aagaacctca tcgaaatctc
     2401 cgctccgagc gcggcaaggg tggaagcgat cgccccccgc gacaatcgcc cgatcgccgg
     2461 gttcgtgccg caggaacgtg tcggcgtgcc ggtcggcggc attacgaaga cgctcggcaa
     2521 cattaccgag aagatggagc gggcgggtga gctggaacgg cagctcgcgg cgggccagat
     2581 gatcatcgaa ctcgacccaa gcctgatcga cgcctctttc gtcagcgacc gcctggcgat
     2641 cgacgctgcc gaactggccg atctcgtcga gcagatccgc gaacacgggc agcaggttcc
     2701 gatcctcgtg cgtccgcatc ctgaagccaa gggtcgttat caggtcgcct acggccatcg
     2761 acggctcgcg gccatcaggg acatcggcat caaggttcgt gcggtcgtgc gcgatctcac
     2821 ggatgaccag ctggtcgtca gccagggaca ggaaaacagc gcccgtacca atctctccta
     2881 tatcgagcgc gcgctcttcg cgtcgcggct cgaggaacgc ggcttcggcc gagatgtcat
     2941 catggcggcg ctcagtgtcg acaaggcggc gctgtcgaga atgctgatcg tcatgcggca
     3001 ggtgccgctc gacctcatca acgccatcgg tgccgcgccc gagatcggcc gccgccgctg
     3061 gctggagctg ggcgatcggc tggagaaagc cgacgtccgg acgatcatcg cggagttgtc
     3121 cgcggacgac gcacgcagga tttcgagcga cgaacgcttc caccgggcat tggttctggc
     3181 gacgaagaag acggccgcgc caaagccggc ggtggccaaa accgaagtca acggcgtgcc
     3241 tgtgatgatc aaaaagacag cagccggcgc tactttcgtc ttcgacggca agacggcgcc
     3301 ggggttcgat cgattcgtcc aggaacggct ccagggcctg ttccaggaat tcaagaagca
     3361 cagaggagcg tagcgcgcaa aagaaaaagg cccccaacga cgccgtcgtg gaagcccttc
     3421 tcagatctta agcacatcga gaatcgcatt tccacgaatc acagtcaagc gtcttaggca
     3481 ccaaattggt ggatggtttt cttttgcctg aacgaaggtg aaggcaatgg agagtggtta
     3541 tgtgacgacg cccttcgggc ggcggccgat gtcgcttggc atgctggcaa gccagcagca
     3601 ggccgggacg atcgagccgg ggatgatgcg cagcaagtgg aagctgtttc gggcaatttg
     3661 cgaggcgcga ccggcgctcg gcgtgaccga tcgggcgctg acggtgcttg atgcgctgct
     3721 gaccttttat cccgacgatg aaatttccga ggagaagggc ctgatcgtct ttccctcgaa
     3781 tgcacagcta tcgctgcgcg cccgcggcat gacgccggcg acgctgcggc ggcacttggc
     3841 cgtcctggtc gaggctggcc tgatcttgcg caaggacagt ccgaacggga aacgctatgc
     3901 ccgccgcgac agggcagggg ccatcggcga agcttacggc ttcagcatcg cgccgcttct
     3961 ggcccgtgcg gtcgagattg aaaatctggc agcccaggca gttgccgacc gggaactgct
     4021 ccgggcgacc agagaacgcc tgacgatctg ccggcgcgat atttccaagc tgatttcggc
     4081 ggccatcgac gaagcggttc ccggtgactg ggagcagatg acggtgatgt ttcgtgctct
     4141 tgtcgcacgg atcccgcggg tggcgagcac cgaagaactg gcatcgctgc tcgaagaaat
     4201 ggggctgctg cgcgacgagg ccgtcaacct gctggaaaga cagataaaaa gagaaaaatt
     4261 ggacgccaat gaatctcaaa ttgagcgcca caaacagaat tcaaaccccg actccactat
     4321 tgaacttgaa ccaagcttcg acacgaagca gggcgaaaag gcagcggcaa acaatgaagg
     4381 ggtggccgag ccgtcggatt cgcaaaggct gaaattgccg aggccttctg ccggatctgg
     4441 tggcaaggcg ccgaatgccg ccaatcctat cgccggccac agcctgaaat ccttcccgct
     4501 cggccttgtc ctgcaggcct gccccgccat tctcgattat gggccgggtg gcgcgatcgc
     4561 caattggcgc gacctgatgt cggccgccgt cgtcgtgcgc tcgatgctcg gcgtcagccc
     4621 ctcggcctat gaggaagcct gcgccggcat ggggccggaa aacgccgcga ccgtgatcgc
     4681 ctgcatcttg gaacggggag gacacatcaa ctcgcccggc ggctatctcc gcgatctcac
     4741 ccgcaggacc gaaaggggcg aattcgcgat tggcccgatg ctgatggcgc tcgtcagggc
     4801 gaatggtggg gcgaagcgcc atgccggata aaggccccag ctacagcgca gttgcgccgt
     4861 tgcctcatct ggtaaactca cctgcttcca gtccgattgg cggctggacg aaagggctcg
     4921 gcgacaggag ccgcccagtc agcgcggcgg cgcagtgctt tctcgtagga tcagctcgac
     4981 cggccataat tcatggacct cctcagcggc gcgtccggcg atcagctgca gcgtcagttc
     5041 ggcgcagcgt gtgcccgcag cacgcatgga agatcgggtt gtcgagagcg acggcaccat
     5101 gttttcggcc gtcagataag ggaagacgtc gtcatgggcg atgacggaaa catcctgtcc
     5161 caccgtcatc ccgagcgagc ggacggcgcg gtaaacccca agcgccgtca tcgtggaacc
     5221 ggcgacgatg gcggtcggtg catttgctct ctcgaggaag gagcgggcat ggcgaaagcc
     5281 gctctcgtcg gagaatttat cgcagatcat catgttgggg tcggcgatga gtccacgctc
     5341 ctgatgggcg agccgaaaac cctgttcgcg atggatcgaa taagtcttgc ccctgaggcc
     5401 attgatcatg gcgatgcggc ggtgcccgag atcgagcagg tgttcggtgg acctgcgcac
     5461 cgcattttca ttatcgatat caagccaggc gtgaacgtgc tccgtctccg accggccgtg
     5521 aaccaggaaa ggcagacgca ggctactcag catctcgatc cgcgggtccc gaggacgagg
     5581 tgagtgaatg atcacactgg attgtatcaa cggaccagtg acagtttctg gctgagacca
     5641 gagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
     5701 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5761 gaaaaaggaa gagtatgagc attcagcatt ttcgtgtggc gctgattccg ttttttgcgg
     5821 cgttttgcct gccggtgttt gcgcatccgg aaaccctggt gaaagtgaaa gatgcggaag
     5881 atcaactggg tgcgcgcgtg ggctatattg aactggatct gaacagcggc aaaattctgg
     5941 aatcttttcg tccggaagaa cgttttccga tgatgagcac ctttaaagtg ctgctgtgcg
     6001 gtgcggttct gagccgtgtg gatgcgggcc aggaacaact gggccgtcgt attcattata
     6061 gccagaacga tctggtggaa tatagcccgg tgaccgaaaa acatctgacc gatggcatga
     6121 ccgtgcgtga actgtgcagc gcggcgatta ccatgagcga taacaccgcg gcgaacctgc
     6181 tgctgacgac cattggcggt ccgaaagaac tgaccgcgtt tctgcataac atgggcgatc
     6241 atgtgacccg tctggatcgt tgggaaccgg aactgaacga agcgattccg aacgatgaac
     6301 gtgataccac catgccggca gcaatggcga ccaccctgcg taaactgctg acgggtgagc
     6361 tgctgaccct ggcaagccgc cagcaactga ttgattggat ggaagcggat aaagtggcgg
     6421 gtccgctgct gcgtagcgcg ctgccggctg gctggtttat tgcggataaa agcggtgcgg
     6481 gcgaacgtgg cagccgtggc attattgcgg cgctgggccc ggatggtaaa ccgagccgta
     6541 ttgtggtgat ttataccacc ggcagccagg cgacgatgga tgaacgtaac cgtcagattg
     6601 cggaaattgg cgcgagcctg attaaacatt ggtaaaccga tacaattaaa ggctcctttt
     6661 ggagcctttt tttttggacg acccttgtca gatcaaagga tcttc
//
