LOCUS       sequence-464663-        6306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-pTi_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK032 [Origin_repABC-pTi_PartType3]
FEATURES             Location/Qualifiers
     source          1..6306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5284..5375
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5376..5444,5445..6236)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6240..6279
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctt tcagttacga tagagttcca cgagaagtta gtgtattggt
      661 cgcgctcttc cccagcgatg atggagacgt cccattcgag acggccaccg aacaccgtcg
      721 cgcgaaggcg gtgcatctgt ttgaggaagc ttcgataccg ttcgtattgg tccggcgaaa
      781 ctgtgagtat ccgcatcgta atctccgcat gaacaggtca tgcgaacaga aatcatctca
      841 cggtgcgttt gcctacgtgc agatttgcac ctcaggtgat tctaccgagt cggtgttcca
      901 aggcgcgaaa tgcgagcggg tgaggccgac cagacgccga caaggttgtg cagatctgca
      961 cttggtgcca cgtcgcacag aagaagggaa tcggtctaac tcacagatag catttgaaga
     1021 atcgggattt agtgtgattt cgattgaaac gcgcgtaacc gttcattaac caaaaacgtc
     1081 ttgcaacctc acccgcatta ggtaatcgtc acggataaat ggcaatacgc gccaattaac
     1141 cgtgacaaga gataacaccg tgagcaaagc cgctgccata tcccgaaatg atcgcccgtc
     1201 ggtagatgtt accattggtg agcatgctga gcagctcagc tctcagcttc aagcgatgag
     1261 cgaggctttg tttcctccga cgtcgcacaa gagcttgcgc aaattcacct cgggtgaagc
     1321 cgcacgcttg atgaaaatat ctgactcaac tcttcgaaag atgacactgg ctggcgaagg
     1381 gccgcaacct gaactcgcca gcaacggacg gcgcttttac accctcggtc agataaacga
     1441 aatccggcag atgcttgccg gctcgactcg aggacgtgaa agcattgatt ttgtgcctca
     1501 tcgccgaggt tctgagcatt tgcaagtcgt tgctgtaacc aacttcaaag gtggctctgg
     1561 gaagacgacg acgtccgctc atcttgcaca gtatctggcg ttgcaaggtt acagggttct
     1621 cgcagtcgat ctcgatccgc aggctagtct ttcagcactc ctcggcgttc tgccagaaac
     1681 tgatgtcggt gcaaacgaaa cgctctatgc ggctattcgg tacgacgaca cacgtcgtcc
     1741 gttgcgagat gtgatccgac cgacgtattt tgatggtctt caccttgttc ctggaaatct
     1801 cgagcttatg gagttcgagc ataccacccc gaaagcattg actgacaaag gtacgcgcga
     1861 cggattgttc ttcactcgcg tggcccaagc ctttgatgag gtcgccgacg attacgatgt
     1921 cgtggtcatc gactgccctc ctcagcttgg gtttttgact ctcagcgggt tgtgtgctgc
     1981 aacatcaatg gtaatcaccg tacatcctca gatgctggat atcgcttcca tgagccagtt
     2041 tctcctcatg acacgcgacc ttctgggtgt cgtgaaagag gcggggggca atctccagta
     2101 cgatttcata cgctatctct tgacgcgcta tgagccccag gacgcgccgc agacgaaagt
     2161 gacggcactg ctgcgcaaca tgttcgagga tcacgtcctt acaaatccta tggtcaagtc
     2221 ggcagcggta tctgatgccg gtttaaccaa gcagacgctc tatgagatag ggcgagagaa
     2281 ccttacgcga tcgacatacg accgggcgat ggaatcttta gatgcggtga attcggagat
     2341 cgaggctttg atcaagatgg cgtgggggcg ggtctaatga aaggctttgc gttcctcaca
     2401 gatctgttgg gagctcccaa cagacaggtg ttgattcgcc ccctggacat ggggcactgg
     2461 agaagccggg gtaatttgag acgacgacgc acgcccatcg ctaattggcc agggtgcagt
     2521 tgtcttgtct tgttgggagc tcccaaccaa gcgcatttgc aatcaaaaat gcgacgccac
     2581 gacgccaaac ccaagaggcc gatatcatga gccgcaaaga cgcaatcgat actttgttcc
     2641 tcaagaagca acctgcgacc gatagagcag cagtcgacaa gtcgaccgct cgtgttcgta
     2701 ccggagcgat ttcggccatg ggttcgtctt tgcaagagat ggctgagggc gcaaaggctg
     2761 cagctcggct gcaggatcaa ctggctacag gcgaagccgt cgtgtccctg gatccatcca
     2821 tgatcgacgg gtcgccgatc gcggatcggc tgccctcaga cgtggatccg aaattcgagc
     2881 agcttgaggc gagcatttcg caggaggggc agcaggtgcc ggttcttgtc agaccgcacc
     2941 ctgaggctgc cggtcgatat cagatcgtat atggaaggcg gcggctgcgc gcggcagtaa
     3001 atctgcggag agaggtttct gccattgttc gaaatctcac ggactgtgaa ctggtcgtgg
     3061 cccagggccg cgaaaatctt aaccgcgctg acctctcgtt cattgagaag gctctcttcg
     3121 ccctgcgcct cgaagatgcg ggttttgata gagccaccat cattgccgcg ctatccactg
     3181 acaaggccga cctcagccgc tacataactg tagcaagggg cataccgctg aacctcgcca
     3241 cacaaatcgg cccagcgtcg aaagcgggtc gatcgcgttg ggtcgtactt gccgaggggc
     3301 ttgggaagcc taaggcaacg gacgcaatcg aagcgatgct tgggtcagag cagttcaagc
     3361 aatctgatag cgatacccgc tttaacctca ttttcaacgc cgtttcaagg ccacctgcga
     3421 agactccaaa aaaggtaagg gcctggagca cgccaaaggg gaaaaaggca gcgacgatcc
     3481 gacaagaaac tggacgaacg gcgctggttt tcgacgagag actggtgcca acttttggcg
     3541 aatatgtcgc tgaccagttg gacagtctgt acgcccagtt cattgaaacc aacggaggag
     3601 gcaagctcga ccaatagtca gggtttcatc caatttaaag ctccgctcga ctgagatgga
     3661 ctggctctca ccgcaaaaga aaaaggcccc cgaaacggcg ttccggaaga ccttctctgt
     3721 agtctcgcag ctaagagaat cgcatttcca ggaatcgtag tcaagggtcc cgtaagggaa
     3781 agcgtcattt cgacgggcgg atttcaattg cctaacaaaa ggtaaaagga aatgcagacg
     3841 catatctcaa cgacgtcctt tgggcggcgg ccgatgacac tcggccatat tgcaagccag
     3901 atggcagcaa aagcggtcgc atcagacact gtcgcccaca aatggcaggt cttccagcac
     3961 atccgtgaat cccggggact gatcggagcc acggaccgct cactctcgat cctgaacgcg
     4021 ctgttgacgt tttacccgga gacagccttg actggtggtg ccgaactggt cgtatggcct
     4081 tctaacgaac agctgatggc tcgcgccaac ggcatgcccg ccacgacact gcgccggcat
     4141 cttgccatac tggttgattg cgggctcatc attcgccgcg acagccccaa tggcaagcgg
     4201 ttcgcccgca agggaagggg aggggagatt gagcaggcct atgggttcga tctgtcgccg
     4261 atcgtcgcgc gggccgagga gttccgagat ctggcccaga cagtgcaagc tgaaaaaaag
     4321 gccttccggg tggccaagga gcgcttgact cttcttcgtc gtgacattgt caaaatgatc
     4381 gaaactggcg tcgaagagag cgttcctgga aactggggaa gagttaccca gacctatcag
     4441 gggatcatcg gccgcctgcc acgctcggca cctcggcagc ttgtcgagag tattgggcaa
     4501 gagcttcagg aactctgcat cgagatccgt gacgtattgg aatctttcac aaaaacgatg
     4561 aatctggacg ccaatgagtc ccatatcggt cgccacaaac agaattcaaa tccagactct
     4621 aaatttgaat ctgaatacag ctctggaaaa aaagatgaag cgggcggcag cgttgcggaa
     4681 accgacaatg tacggagctt gccgaaacgc gagctgcctt tgggaatcgt gctggatgcc
     4741 tgccccgaaa tgcgggaatt ggcccaggga ggtccaattc ggcattggcg cgacttgctg
     4801 gcggcggctg agcttgcccg gccgatgctg gggattagtc cgagcgcctg gcgggaggcc
     4861 cgcgaaacca tgggcgatca acacgcggcg atcacgctgg cttcgatcta tcagcgggcc
     4921 ggtcagatca ataacgctgg gggctatctg cgcagcctga ccgaccgggc caaggatggg
     4981 aagttttcga cctggccgat ggtcatggcg ttgctccggg caaagctgga cgagcagaag
     5041 aatgcagttg gcgctggaaa gccgcgaact gctgaggagg tcgaggatga cagccgcctc
     5101 cacgtatcgg aatcgctgct caaaaacctg cgaaagccga gatcttggtg atcctctcgc
     5161 tattcagccg cggcgatgtc gacgtcggtg atcaacactg gattgtatca acggaccagt
     5221 gacagtttct ggctgagacc agagtagccc gcctaatgag cgggcttttt tttaattccc
     5281 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5341 gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg
     5401 cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg
     5461 tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc
     5521 tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca
     5581 cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac
     5641 tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa
     5701 aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg
     5761 ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt
     5821 ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg
     5881 aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc
     5941 gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga
     6001 tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta
     6061 ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc
     6121 cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg
     6181 atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg
     6241 atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc agatcaaagg
     6301 atcttc
//
