LOCUS       sequence-464664-        6193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-pSymB_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK033 [Origin_repABC-pSymB_PartType3]
FEATURES             Location/Qualifiers
     source          1..6193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5171..5262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5263..5331,5332..6123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      6127..6166
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctg ccttatcctt ctcttatccg acctgcgggg ctcgatcctg
      661 tcacaatccg gtccgctgtc aatagtctac taaaattatg atctactaag ggtaccgcgt
      721 ctgttgcaac gtctggggga gatttgcgcg gccgctcgga tgcggcgctt cgccaacgca
      781 agtgtcggcc ggatcggccc ggctggactg ccggcgagtt gtccgcggac aaccaatgaa
      841 cagccggacg acaaaactgc agcgcccctg cagcacaacc gagctttttg ttaagcgttt
      901 gtaaaatggc aacgaatcgg cgcatagtat aactgcgcag atcagctccc attggtgaat
      961 tttgcgcagt ttttggagag taggaatgtt gcagcagaga atccagagtc ttgaggagca
     1021 ggagcatttg ccgtcgctct tgtctgccga cgctcaggaa ctcgcacacc agttgcagga
     1081 gcatcaaaag aagatctttt ctccgtcggc gcaaaagacg atgcggctct tcagcccggc
     1141 agaggcagcg gccttcatcg gaatcggcga aggctatctg cgccagatcg cctcagaagg
     1201 ccatggtccg cagccgctgt caaacggccg ccgcatgtat gcgatggagg acatcgaccg
     1261 tatccgccgg gttctcgacg aaggcagcaa gaccggcaaa tatattccgc atcgtcgacc
     1321 gggcgaaaag ctgcaagtcg tctccgtcat gaatttcaaa ggtggttcgg gcaagacgac
     1381 gacatccgct caccttgcgc agtatctcgc cttgcgcggc tatcgggtgc tggcgatcga
     1441 tctcgatccg caggcctcgc tctccgccct cttcggtcat cagccggaac tggacgtcgg
     1501 cgaaggggaa acactctatg gcgccatacg ctacgaggcc ccccggccga tcgcggaggt
     1561 ggtgcgatcg acctataccg ccaatcttca cctcattccg ggcaatctcg agctcatgga
     1621 attcgagcac gagactccga aggcgatgat cgcgggctcc atcgagacaa tgttcttcgc
     1681 gcggatcggc gaggtgcttt ccgaaatcga gagcttctac gacatcgtcg tcatcgactg
     1741 cccgccgcag ctcggctttc tgaccatgtc ggcgctttgc gccgcgacct ccgtgctcat
     1801 caccgtgcac ccgcagatgc tcgacgtcat gtcgatgtcc cagttcttgt cgatgacgag
     1861 cgagctcatg gccgttgtcg agaatgccgg cgggcgcacc agctatgact ggatgcgcta
     1921 tctggtcacg cgcttcgagc ccaacgacgg accgcagagc caaatgaccg gtttcatgcg
     1981 cgcgatcttc ggcaaccgca tgctgcagaa cgcgatggtg aaatcgaccg caatttccga
     2041 cgccggcgtc accaagcaga cgctctatga agtggagcgc tcgcaattca tacgcggtac
     2101 ctacgaccgg gcgatggatt cgctctcgct cgtcaatgcg gagatcgaag agatgatccg
     2161 cagggtctgg gggaggaagt aaccgatggc gcgcaagaac ctgatcggca tttccgagag
     2221 ccccctcgcc cccgaagacg gggagcgccc ggccgtcggt cgtccgatcg cgggccttgc
     2281 gcccgcccag cgctccactg gcctcgtcgg cggcatcacc agatccctct cgaacatcac
     2341 ccagaaggtc gagcgagcgg aggaattgga gcgccagctt gccgatgggc acgcaatcgt
     2401 cgaactggac ccggagctga tcgattcctc tttcatcatc gataggctgg gtgtcgcccc
     2461 cgacgttcag gccatgctgg ttcagcagat tcgggaccac ggacaacagg ttccgatcct
     2521 cgtgcggccg catccggcgg agagcggccg atatcaggtc gcctacggcc acaggcggct
     2581 tgcggcattg cgcgagatcg gcatcaaggt caaggcagtc atccggaact tgaacgacga
     2641 gcaactcgtc atttcccagg ggcaggaaaa caacgcccgc accgacctct ccttcataga
     2701 gcgagcgttg ttcgcaacgc gtcttgaaga tcgcggcttc tcgcgggata cgatcatgtc
     2761 ggccctcgga gtcgacaagg cggccctctc caagatgatc gccgtcgtcc gccgattgcc
     2821 gttggacatc atcgaagcca tcggtgccgc gccgggtttc ggcaggagac gatggatgga
     2881 acttgccgat ctcatcgacc gcgatgaccg gcgtccgaag gcattgcatt tcatcgagac
     2941 ggccgcattc aaggcgctgg agagcgatca gcgtttcgaa aagctgtttg ccttgctgag
     3001 cctagccagg aagccctcaa aggcggaaac ctggattgct ccggacagga gccgccccgt
     3061 gcgtattcgc gaaacggatg cggaaacgac gctggccttc agcaagaagg cggcaccggg
     3121 ctttgcagaa ttcgtgaggc agaggctcga agcgctctac ctcgaatatc aacgggaaac
     3181 aggagactaa acagagcaaa agaaaaaggc ttccgaacga ctagcgctgc ggaaaccctt
     3241 ctctcgtgta gcaactagag aatcccactt ccgcgaatca gtgtcaagca tttttgacgt
     3301 cgtttcggcg aacggatttc ttttgccttg gaaaaggtga agaaaatgga acgtggaagt
     3361 gtgacgacgc cctttgggcg gcgggcgatg acgcttggca tgctcgcaag tcagatcgcc
     3421 gccggtaacg tcccgccgga ccggtcggtg gacaagtgga aattgttccg ttctctctgt
     3481 gaagcgaaga cggtgatcgg cgtgtccgat cgttcgcttg cggtgttgaa cgcccttttg
     3541 agcttttatc caggagccga gctctcgggc gaaaatggcc tcgtcgtttt cccctccaat
     3601 gcgcagctct cgctccgtac ccacggaatg gcgggcacga cgctccgccg gcatcttgca
     3661 tgcctcgtgg aatcgggcct gatcctgcgg cgcgacagcc cgaacggtaa gcgttacgcc
     3721 cgaaggagcc gtaatggagc ggtgggcgaa gccttcggat tcgacctctc cccgctcgtc
     3781 gcccgggccg atgagttcaa agccgccgct gccgaacttg ccgcagccaa acagtatcat
     3841 cgtctgatgc gcgaaagact gagcctctgc cggcgcgaca tcgccaagct gctcgagctc
     3901 cttcgtgact ctgacctgcc caccgactgc gagcggcagc aacggagata tgaggaattg
     3961 tctgcgcctc tctcgcgcaa ggcaccggtc agcgacgtcg aaacccttca tgacgctatg
     4021 gcggaacttc gtacggatct atccaatcaa ttggaaaaac tgcttaattc caaaaaaatg
     4081 ggcgccaatg atcgccataa tgggcagcac atacagagtt cacatccaga ctctcaattt
     4141 gaatctgaac ctggcttggg aaaagggtcg gggaaatttt cggccggcgc ccaaagctgc
     4201 aacgcggacc ctgtgacacc gacggcgaag ctctcggagg tttcgacccg tatcgattcg
     4261 cagcagccgg tcagctcggt gtccccatct ctgccgatct cccgcctcgc ccgggaaatt
     4321 cctctgcccg tcctcctatc cgcttgcccg cagatcgccg attacggacc acagggccgt
     4381 ataacgaact ggaaggatct gctgaccgct gcagtcgtcg tcagaaccat gctgaacgtg
     4441 agcaccgatg cctatgacga tgcatgcacg gttctcggcc aggaaaatgc tgcgaccgtc
     4501 atcgcttgca tcctggagag agccgattgc ataagttcgc caggcggcta tcttcgggtt
     4561 cttaccgcga gagcgcggcg ccaggctttc tcggttgctc cgatggtcgc tgcactgagg
     4621 cgcgcgcgag gggcaagcat gccgcttgcg agttgatgtc tccgctgaac agtctttgta
     4681 agacaggaat ctcgggcgca ctgcaggagt tgtcggtcgc aaggcacgtc aggcttgcga
     4741 cgctgaccgc tcgcggttgc cttgacgctc cagatagggg aaactcgctt cggctgcgga
     4801 ttcgcacttg ctttgaattg ccgccttggt gtctgtttct taacaatttt gcttctatcg
     4861 taacgtattg gcaaaaaagg cggtaagccg gcaatgcgga agttaaatcg ccattaagtt
     4921 ccgtcacgcg gacggcccca gttcgaggct tggtgaccgc aacaacagtt atcaaccgcc
     4981 tcccctattc tgcaattgag tggaaatctc gtgcggtcgg ccacgcaatc cggggcaaag
     5041 ccgccgacga tatgcagatg ccgatccctg cagtcgtttc cgcagttgac agctgtcaac
     5101 aatacgatct tgcccctggc tgagaccaga gtagcccgcc taatgagcgg gctttttttt
     5161 aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt
     5281 cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa
     5341 accctggtga aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa
     5401 ctggatctga acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg
     5461 atgagcacct ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag
     5521 gaacaactgg gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg
     5581 accgaaaaac atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc
     5641 atgagcgata acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg
     5701 accgcgtttc tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa
     5761 ctgaacgaag cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc
     5821 accctgcgta aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt
     5881 gattggatgg aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc
     5941 tggtttattg cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg
     6001 ctgggcccgg atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg
     6061 acgatggatg aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg
     6121 taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcaga
     6181 tcaaaggatc ttc
//
