LOCUS       sequence-464665-        6031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Origin_repABC-pSymA_PartType3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK034 [Origin_repABC-pSymA_PartType3]
FEATURES             Location/Qualifiers
     source          1..6031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5009..5100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5101..5169,5170..5961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      5965..6004
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctccctg aaccatccgg tgcagtcgtt gctcggtcat ggccaacgag
      661 attgaggcag gccggagcgc aggcgagcac cgagatgccg agctgttccc aatacggcac
      721 atatgtctcg ccttggcgag cagtcggtac tgcgcgccag cgtcgtcgct tcgagagttc
      781 cccgcgggga actctgcgaa gagttcggca gatctccatt gaaagcaacg gccaaccatt
      841 tccgcagtga accactattg cgtctccgcc cagcgacgtc tgcgcacatc ttcgtccaaa
      901 aaagaggcgg ggagggtcga gaaccggcat gcactggcgc cttccccccg gctctgtgcg
      961 tcatccacag cgcgcattga tggccatagc cgaatcggag tacgtcttag ttgcggaaat
     1021 ttttcgaatt tgcgtgtttt cgagtagtta cgtggcgatt agcaagcggt taaccacttc
     1081 actgcttaag tgacggaaac gatcacaggg attctttcga tgacgcgaca cctaagcagc
     1141 ctcgctttca tggagaattt ttccagtaag ctcgaaatgg cactgaacaa cttgagcatg
     1201 gcgcagttcc ccccgaacgc gcgtcgcaca atgcgcaagt tcacctctac cgaagttgcc
     1261 accctgctgg gcgtaaccga ggcctatatc cgccaggttg ccgccaagga acagggccct
     1321 gagcccgaaa tcgccaacgg ccggcgcttc tacacgctcg agcaggttct tgagctacgt
     1381 atggctcttg cggcaaacgg ccggaagaaa tggatgaacc cccgccggac aggcaatgag
     1441 caatgccaga tcgttgcggt caccaacttc aagggcggct ccagcaagac ctctaccaca
     1501 atccatctcg gtcactatct ggcactcaag ggctatcgcg tgctggcggt cgatctcgac
     1561 ccgcaagcat ctctcaccgc gctccatggt agcctgcccg aattcgatta ccgcggcggc
     1621 gatacacttt tctcagccat tcgtttcgac gatcccgtcc cgacgaaatc catcatccac
     1681 aagacccaca tcgttggttt cgacgtcatc tgtgccggcc tcgaactgac cgagttcgag
     1741 acggccgtcg ccctcgagat gcggcggtca gcaggaaccg gtttcttgct tcgcgtttct
     1801 caagctctgg agcaggtcgc cgatgactac gacttcgtcc ttatggactc ggctccgtcg
     1861 ctgaacttcc tgacactttc gtcgctgacc gccgcgactg gagtcatcat cccggttccg
     1921 gcacatatgc tggatgtgga ctcgaccgca aagtttttgg agcttgccgg gtcgtacatg
     1981 cagatcctga atgaggtggg caccgccgct caatgggact tcgccaagtt cctgatcaca
     2041 aagttcgagc cgaacgatca tccgcaagcg aatatgtccg cgctgatgag acaggtgttc
     2101 ggcgaggatc tgctcctcaa ctccgtcatc aagtcgaccg cggtggccga tgctctgacg
     2161 tggaagcaga gcctttacga ggttcagagg tcccgatttt cggcgccgaa aacctacgac
     2221 cgcgccatcg agtcgatcaa tgctgccaat gctgaactcg agggcctctt ctggaaggca
     2281 tgggggagag aatgagccgc aaggattcca agggcctttt tgccaatgtc ctcggacagc
     2341 tggaaaattc tgcagagaag ggcgggatgc agcggtcgac atcgccacat cttctcaaag
     2401 ttgcagccgg cgtccgtcag atgcaggaac gcagcgaact tgccgaacgc cttctcaagg
     2461 atggtggcca gatcgtcgag atcgatccag acgagatcat ggaatcggcc atccgggatc
     2521 gctttgattc cggctacagc gaagccggta ttgcggattt gctcgagtcg atgcgcgagc
     2581 atggacagag tactcctggc ttggtccgcc cggttcgagg ggccgcaagg ccgtttcaga
     2641 tcgtatttgg ccggcgccgg cttgccgctg ccaaactcct cggcatcaag ttcaaggcaa
     2701 tcgcccggga acttagcgac gaagatgcga tcgttctcca aggggaagag aacagcaacc
     2761 gcaaggacct gtccttcatc gagcgatgcc tttttgcgca atcgcaggag gctgccggct
     2821 atcgccgcga cgtgatctgc aagtccctga gcaccggcag gtcgcatatc tccgaaatga
     2881 tccggatagc ggcagcattg ccgcgcgaga tcctgatgca gattggtccg gctcctgaaa
     2941 tcggacggcg tagatggatc gagttcgaag tgcgctgggc ggctcaccgg gagcccgcga
     3001 aggtggcgca gttggttctg gagcaagagc agattcaagc ctcttccagc gatctacgct
     3061 tcactgcggt ttttgaagcc cttaccaagg ttgatgtgag ggctgcagcg tcttccacga
     3121 gcgatctgat ctctcatggc ctcgtgcttg gccagatcca acgcggcaag tcggctgcga
     3181 agctgacatt caacaagtct gtgccgtcag gctttgtcga tttcattgca ggtcagatcg
     3241 aaagcctgca cgaccagttc atgcaaaagc agagttcgaa acaaggagac taacccgcaa
     3301 aagaaaaagg cccccaacga acaagccgtg gaagccttcc tcagatttgt agcaagatcg
     3361 agaatcgcat ttccacgaat cacagtcaag agtccttggc accgtttttg gtgagctgct
     3421 ttcttttgcc ttatcgaagg tgaaggaaat ggagattgga agtgtgacga cgccctttgg
     3481 gcggcggtcg atgacgcttg ccctgttggc aggccagttc atctcccgcg acatcgaacc
     3541 tggcaagtcg gtcgacaagt ggaaattgtt tcgctcgctt tgtgctgcca aacgccgtct
     3601 tggcatcagc gatcggacac tagccgttct gaacgcgctg ttgagcttct atccggagaa
     3661 cacgctaagc ggggagacaa gtctggtcgt ctttccctcg aacgttcagc tgtcactgag
     3721 agctcatggc atggccgagg ctacgctcag aaggcatctg gccgccctcg tcgatgcagg
     3781 gctggtcgct cgcaaggaca gtccgaacgg caagcgctat gcacgcaaga ctcgcgatgg
     3841 agcgatcggc acagcgttcg gcttttcgct cgcaccactg ctggcccgct ccggcgaaat
     3901 agagcgactc gctgccgaag ccgaggctga tcggatggag ctgcagcggc tacgcgagcg
     3961 actgacgctc tgccgcagag acatcgccaa gttgatagac gtggtgctcg tagaagtgcc
     4021 gggccactgg acgtctatgc aggaagattt ccgaagcctc gtccaggcga taccgaggac
     4081 gcccgatatc gctgatctca cccctttggt agtggcattc gaaacgctgc gcgaacaggt
     4141 ggctaaccag ttgcaaaagc acataaaacc acacgagatg agcggcaatc ccgatcaaac
     4201 tgagcggcac aaacaggaat caaaacccga atctattatt gaacttgaac gttgctctcg
     4261 gaatgagcgg ttagacggcc aagccattga accggccaac aaggcaaaaa gggcctttcc
     4321 gctgggaatg gtactgaaag cctgcccgga aatcatgaac tacggtcctg acggtgcagt
     4381 gagaagttgg cgcgacctca tgatcgctgc agtcacggta cgatctatgc ttggcgttta
     4441 cccctcggcc tatcaggagg cctgcgaggt catgggaccg gaaaacgccg gagtcgccgt
     4501 ggcctgcatt ctagagaggg cagggcatat caactctgct ggcggctatc tgcgcgatct
     4561 gaccgccaag gcgcggcaag ggaaattctc gcttggcccg atgctcatgg cgttgctgag
     4621 agcgaatggc ttcgatgggc ggggggtgtc gtgataccgg ccgcctgacc aacaccgtgg
     4681 ggacacaatc ggcacgggca tatgccgacc cgcgggtgcc gagcgatccc actcaagcgg
     4741 aatcgccagc ggcatccacc cctgagcgtc gggcgtgctc caaattcgtc ggtcgccgca
     4801 cttcgtaatc agcgccggac ccccctggct accttccagc gatgtgccta catcccggta
     4861 caatttcggg ggtttcgcaa acattcccga tacgtagccc aggcaaaaca cacgtcgtca
     4921 aatgtcgcga tcgtgcgacg ccacgaacgg agtgtggctg agaccagagt agcccgccta
     4981 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
     5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5101 atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg
     5161 gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg
     5221 cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg
     5281 gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc
     5341 cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg
     5401 gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg
     5461 tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt
     5521 ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg
     5581 gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg
     5641 ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca
     5701 agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt
     5761 agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc
     5821 cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat
     5881 accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg
     5941 agcctgatta aacattggta aaccgataca attaaaggct ccttttggag cctttttttt
     6001 tggacgaccc ttgtcagatc aaaggatctt c
//
