LOCUS       sequence-464669-        2142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_sacB-Bacillus_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK039 [Con-Pro_sacB-Bacillus_PartType5]
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        620..1065
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     promoter        1120..1211
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1212..1280,1281..2072)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2076..2115
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacgc acatatacct gccgttcact attatttagt gaaatgagat
      661 attatgatat tttctgaatt gtgattaaaa aggcaacttt atgcccatgc aacagaaact
      721 ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta ggcccgtagt
      781 ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca gttgcaatcc
      841 aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt tactgataaa
      901 gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca ccccttacat
      961 attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag gagggctgga
     1021 agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgtatgt gagaccagag
     1081 tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac
     1141 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1201 aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt
     1261 tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc
     1321 aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat
     1381 cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg
     1441 cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc
     1501 agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg
     1561 tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc
     1621 tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg
     1681 tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg
     1741 ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc
     1801 tgaccctggc aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc
     1861 cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg
     1921 aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg
     1981 tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg
     2041 aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga
     2101 gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
