LOCUS       sequence-464672-        1912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_acc3-Broad_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK042 [Con-Pro_acc3-Broad_PartType5]
FEATURES             Location/Qualifiers
     source          1..1912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(620..624,625..641,642..647)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        890..981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(982..1050,1051..1842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1846..1885
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacgt gacataagcc tgttcggttc gtaaactgta atgcaagtag
      661 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      721 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agcctatgcc tcgggcatcc
      781 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgtatgt
      841 gagaccagag tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt
      901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      961 taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt
     1021 tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat
     1081 gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa
     1141 attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg
     1201 ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt
     1261 cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat
     1321 ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg
     1381 aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg
     1441 ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac
     1501 gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg
     1561 ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga agcggataaa
     1621 gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc
     1681 ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg
     1741 agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt
     1801 cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc
     1861 tccttttgga gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
