LOCUS       sequence-464674-        1896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_ipxC-Xantho_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK044 [Con-Pro_ipxC-Xantho_PartType5]
FEATURES             Location/Qualifiers
     source          1..1896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        874..965
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(966..1034,1035..1826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1830..1869
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacga agcaagcgtg atttggcgtc accccccgtg acgactaggg
      661 tgcacccatc ctgagggacc gactcgccga ccgtgttgac aatttctttg tacaaccggg
      721 gcgaaagttg ggacccaagg tgaaggtttg agggaaggag tgcggcaagc gggacacacc
      781 cgttagccgg aaacttcccc gaaacgggac gcaccaaagt atgtgagacc agagtagccc
      841 gcctaatgag cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa
      901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      961 agagtatgag cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc
     1021 tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg
     1081 gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc
     1141 gtccggaaga acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc
     1201 tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg
     1261 atctggtgga atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg
     1321 aactgtgcag cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga
     1381 ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc
     1441 gtctggatcg ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca
     1501 ccatgccggc agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc
     1561 tggcaagccg ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc
     1621 tgcgtagcgc gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg
     1681 gcagccgtgg cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga
     1741 tttataccac cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg
     1801 gcgcgagcct gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt
     1861 ttttttggac gacccttgtc agatcaaagg atcttc
//
