LOCUS       sequence-464677-        1886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_murB-Xantho_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK048 [Con-Pro_murB-Xantho_PartType5]
FEATURES             Location/Qualifiers
     source          1..1886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        864..955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(956..1024,1025..1816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1820..1859
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacgc ttcctcggcc cagcgttact tggtggtggt gagcttggtg
      661 tcggggggga gcgcgtcttc gctcatggtg gggcagctcc aggcgaacgg agccccatga
      721 tgccacggcc gtggccggtg gggaaatcgc ccggcttgcc ctgtcgccgc atgtgtggga
      781 gaggcacgcc cgaaggaccg gtgacaccct atgtgagacc agagtagccc gcctaatgag
      841 cgggcttttt tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      961 cattcagcat tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt
     1021 tgcgcatccg gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt
     1081 gggctatatt gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga
     1141 acgttttccg atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt
     1201 ggatgcgggc caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga
     1261 atatagcccg gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag
     1321 cgcggcgatt accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg
     1381 tccgaaagaa ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg
     1441 ttgggaaccg gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc
     1501 agcaatggcg accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg
     1561 ccagcaactg attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc
     1621 gctgccggct ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg
     1681 cattattgcg gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac
     1741 cggcagccag gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct
     1801 gattaaacat tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac
     1861 gacccttgtc agatcaaagg atcttc
//
