LOCUS       sequence-46468-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Israel MA, Yuan SH, Bardy C, Reyna SM, Mu Y, Herrera C, Hefferan MP,
            Van Gorp S, Nazor KL, Boscolo FS, Carson CT, Laurent LC, Marsala M, 
            Gage FH, Remes AM, Koo EH, Goldstein LS
  TITLE     Probing sporadic and familial Alzheimer's disease using induced 
            pluripotent stem cells.
  JOURNAL   Nature. 2012 Jan 25;482(7384):216-20. doi: 10.1038/nature10821.
  PUBMED    22278060
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-01-25- 25"; volume: "482"; issue: "7384"; 
            pages: "216-20"
COMMENT     Sequence Label: pCX4-EGFP
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(37..39,40..42,43..555)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(82..103)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(343..362)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccatcctcta gactgccgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
       61 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      121 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      181 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      241 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      301 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      361 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      421 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      481 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      541 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      601 atcggcgacg gccccgtgct
//
