LOCUS       sequence-464682-        2452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Inducible-Promoter_tetAR-Broad_PartType5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK054 [Ind-Pro_tetAR-Broad_PartType5]
FEATURES             Location/Qualifiers
     source          1..2452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(620..1270)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     promoter        1430..1521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1522..1590,1591..2382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2386..2425
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaacgt caatcgtcac cctttctcgg tccttcaacg ttcctgacaa
      661 cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg aacgctgcgt
      721 ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag agcggagcct
      781 gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc tcaagcacgg
      841 ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg gccgaaaaac
      901 ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt gcgcccggtc
      961 gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc ctgaagctgc
     1021 gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc gaatgcgtat
     1081 gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg ttcctgaagt
     1141 gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt gtcgtcagac
     1201 cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta ttcggctgca
     1261 actttgtcat gcttgacact ttatcactga taaacataat atgtccacca acttatcagt
     1321 gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc cagactatgt
     1381 gagaccagag tagcccgcct aatgagcggg ctttttttta attcccctat ttgtttattt
     1441 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1501 taatattgaa aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt
     1561 tttgcggcgt tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat
     1621 gcggaagatc aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa
     1681 attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg
     1741 ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt
     1801 cattatagcc agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat
     1861 ggcatgaccg tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg
     1921 aacctgctgc tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg
     1981 ggcgatcatg tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac
     2041 gatgaacgtg ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg
     2101 ggtgagctgc tgaccctggc aagccgccag caactgattg attggatgga agcggataaa
     2161 gtggcgggtc cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc
     2221 ggtgcgggcg aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg
     2281 agccgtattg tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt
     2341 cagattgcgg aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc
     2401 tccttttgga gccttttttt ttggacgacc cttgtcagat caaaggatct tc
//
