LOCUS       sequence-464683-        1951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_rpsM-Xantho_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK055 [Con-Pro_rpsM-Xantho_PartType8]
FEATURES             Location/Qualifiers
     source          1..1951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        929..1020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1021..1089,1090..1881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1885..1924
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga ggcgacggag cagctttacg cgctggtgag gcgctgatcc
      661 ctctcgccct tgacccatca cggaatctcc gctatgggca ctccctcgct tcggaacagg
      721 tgccgttcgg tccacccgaa agggtcgggc agggcggtgg ctttgtcgta tccggggcag
      781 cattcgtcaa gatcccgcag gggccggcct ccaccggacg cggggacaac aaccggccag
      841 ccgccttcgg gcgcaggccc acatggagag cgcaaatgtg agaccagagt agcccgccta
      901 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
      961 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1021 atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg
     1081 gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg
     1141 cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg
     1201 gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc
     1261 cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg
     1321 gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg
     1381 tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt
     1441 ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg
     1501 gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg
     1561 ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca
     1621 agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt
     1681 agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc
     1741 cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat
     1801 accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg
     1861 agcctgatta aacattggta aaccgataca attaaaggct ccttttggag cctttttttt
     1921 tggacgaccc ttgtcagatc aaaggatctt c
//
