LOCUS       sequence-464685-        1938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_rpsL-Xantho_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK057 [Con-Pro_rpsL-Xantho_PartType8]
FEATURES             Location/Qualifiers
     source          1..1938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        916..1007
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1008..1076,1077..1868)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1872..1911
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgg ataacttcag gtgcatgatc gcggcgccgg ccgggaaaaa
      661 agccccggta tagtgcgctg tgatcctctg tcgcgagaaa ggcgtgtctg cccccgtttg
      721 ggcggtggac ggcgcctttc gtgttcgtcg gtgcgaaggg aattcaggcc ccctcggggc
      781 cagtcgtggc gatccttcac aggggtcgtc gaaatgaagg atgcggccaa ggccgcagat
      841 caggacgagg cgaaggcgta caatgtgaga ccagagtagc ccgcctaatg agcgggcttt
      901 tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agcattcagc
     1021 attttcgtgt ggcgctgatt ccgttttttg cggcgttttg cctgccggtg tttgcgcatc
     1081 cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc gtgggctata
     1141 ttgaactgga tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa gaacgttttc
     1201 cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt gtggatgcgg
     1261 gccaggaaca actgggccgt cgtattcatt atagccagaa cgatctggtg gaatatagcc
     1321 cggtgaccga aaaacatctg accgatggca tgaccgtgcg tgaactgtgc agcgcggcga
     1381 ttaccatgag cgataacacc gcggcgaacc tgctgctgac gaccattggc ggtccgaaag
     1441 aactgaccgc gtttctgcat aacatgggcg atcatgtgac ccgtctggat cgttgggaac
     1501 cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac caccatgccg gcagcaatgg
     1561 cgaccaccct gcgtaaactg ctgacgggtg agctgctgac cctggcaagc cgccagcaac
     1621 tgattgattg gatggaagcg gataaagtgg cgggtccgct gctgcgtagc gcgctgccgg
     1681 ctggctggtt tattgcggat aaaagcggtg cgggcgaacg tggcagccgt ggcattattg
     1741 cggcgctggg cccggatggt aaaccgagcc gtattgtggt gatttatacc accggcagcc
     1801 aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc ctgattaaac
     1861 attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg acgacccttg
     1921 tcagatcaaa ggatcttc
//
