LOCUS       sequence-464687-        1815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_cat-Broad_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK059 [Con-Pro_cat-Broad_PartType8]
FEATURES             Location/Qualifiers
     source          1..1815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        636..738
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        793..884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(885..953,954..1745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1749..1788
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
      661 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
      721 ggagctaagg aagctaaaaa tgtgagacca gagtagcccg cctaatgagc gggctttttt
      781 ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt
      901 ttcgtgtggc gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg
      961 aaaccctggt gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatattg
     1021 aactggatct gaacagcggc aaaattctgg aatcttttcg tccggaagaa cgttttccga
     1081 tgatgagcac ctttaaagtg ctgctgtgcg gtgcggttct gagccgtgtg gatgcgggcc
     1141 aggaacaact gggccgtcgt attcattata gccagaacga tctggtggaa tatagcccgg
     1201 tgaccgaaaa acatctgacc gatggcatga ccgtgcgtga actgtgcagc gcggcgatta
     1261 ccatgagcga taacaccgcg gcgaacctgc tgctgacgac cattggcggt ccgaaagaac
     1321 tgaccgcgtt tctgcataac atgggcgatc atgtgacccg tctggatcgt tgggaaccgg
     1381 aactgaacga agcgattccg aacgatgaac gtgataccac catgccggca gcaatggcga
     1441 ccaccctgcg taaactgctg acgggtgagc tgctgaccct ggcaagccgc cagcaactga
     1501 ttgattggat ggaagcggat aaagtggcgg gtccgctgct gcgtagcgcg ctgccggctg
     1561 gctggtttat tgcggataaa agcggtgcgg gcgaacgtgg cagccgtggc attattgcgg
     1621 cgctgggccc ggatggtaaa ccgagccgta ttgtggtgat ttataccacc ggcagccagg
     1681 cgacgatgga tgaacgtaac cgtcagattg cggaaattgg cgcgagcctg attaaacatt
     1741 ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg acccttgtca
     1801 gatcaaagga tcttc
//
