LOCUS       sequence-464691-        1829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_bla-v2-Broad_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK064 [Con-Pro_bla-v2-Broad_PartType8]
FEATURES             Location/Qualifiers
     source          1..1829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        661..752
                     /gene="bla"
                     /label=AmpR promoter
     promoter        807..898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(899..967,968..1759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1763..1802
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga gtagcccgcc taatgagcgg gctttttttt aattccccta
      661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      721 aaatgcttca ataatattga aaaaggaaga gtaatgtgag accagagtag cccgcctaat
      781 gagcgggctt ttttttaatt cccctatttg tttatttttc taaatacatt caaatatgta
      841 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      901 gagcattcag cattttcgtg tggcgctgat tccgtttttt gcggcgtttt gcctgccggt
      961 gtttgcgcat ccggaaaccc tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg
     1021 cgtgggctat attgaactgg atctgaacag cggcaaaatt ctggaatctt ttcgtccgga
     1081 agaacgtttt ccgatgatga gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg
     1141 tgtggatgcg ggccaggaac aactgggccg tcgtattcat tatagccaga acgatctggt
     1201 ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg
     1261 cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg
     1321 cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga
     1381 tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc
     1441 ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag
     1501 ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag
     1561 cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg
     1621 tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac
     1681 caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag
     1741 cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
     1801 gacgaccctt gtcagatcaa aggatcttc
//
