LOCUS       sequence-464693-        2051 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_nptII-Broad_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK066 [Con-Pro_nptII-Broad_PartType8]
FEATURES             Location/Qualifiers
     source          1..2051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1029..1120
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1121..1189,1190..1981)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1985..2024
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctga gcttcacgct gccgcaagca ctcagggcgc aagggctgct
      661 aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
      721 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
      781 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
      841 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
      901 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat
      961 gaggatcgtt tcgcaatgtg agaccagagt agcccgccta atgagcgggc ttttttttaa
     1021 ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1081 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagcattc agcattttcg
     1141 tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac
     1201 cctggtgaaa gtgaaagatg cggaagatca actgggtgcg cgcgtgggct atattgaact
     1261 ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat
     1321 gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga
     1381 acaactgggc cgtcgtattc attatagcca gaacgatctg gtggaatata gcccggtgac
     1441 cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg cgattaccat
     1501 gagcgataac accgcggcga acctgctgct gacgaccatt ggcggtccga aagaactgac
     1561 cgcgtttctg cataacatgg gcgatcatgt gacccgtctg gatcgttggg aaccggaact
     1621 gaacgaagcg attccgaacg atgaacgtga taccaccatg ccggcagcaa tggcgaccac
     1681 cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca agccgccagc aactgattga
     1741 ttggatggaa gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc cggctggctg
     1801 gtttattgcg gataaaagcg gtgcgggcga acgtggcagc cgtggcatta ttgcggcgct
     1861 gggcccggat ggtaaaccga gccgtattgt ggtgatttat accaccggca gccaggcgac
     1921 gatggatgaa cgtaaccgtc agattgcgga aattggcgcg agcctgatta aacattggta
     1981 aaccgataca attaaaggct ccttttggag cctttttttt tggacgaccc ttgtcagatc
     2041 aaaggatctt c
//
