LOCUS       sequence-464696-        1796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Constitutive-Promoter_neo-Broad_PartType8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK069 [Con-Pro_neo-Broad_PartType8]
FEATURES             Location/Qualifiers
     source          1..1796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        774..865
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(866..934,935..1726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1730..1769
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctgctgt gtgtctcaaa atctctgatg ttacattgca caagataaaa
      661 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      721 atgtgagacc agagtagccc gcctaatgag cgggcttttt tttaattccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag cattcagcat tttcgtgtgg cgctgattcc
      901 gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg gaaaccctgg tgaaagtgaa
      961 agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt gaactggatc tgaacagcgg
     1021 caaaattctg gaatcttttc gtccggaaga acgttttccg atgatgagca cctttaaagt
     1081 gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc caggaacaac tgggccgtcg
     1141 tattcattat agccagaacg atctggtgga atatagcccg gtgaccgaaa aacatctgac
     1201 cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt accatgagcg ataacaccgc
     1261 ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa ctgaccgcgt ttctgcataa
     1321 catgggcgat catgtgaccc gtctggatcg ttgggaaccg gaactgaacg aagcgattcc
     1381 gaacgatgaa cgtgatacca ccatgccggc agcaatggcg accaccctgc gtaaactgct
     1441 gacgggtgag ctgctgaccc tggcaagccg ccagcaactg attgattgga tggaagcgga
     1501 taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct ggctggttta ttgcggataa
     1561 aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg gcgctgggcc cggatggtaa
     1621 accgagccgt attgtggtga tttataccac cggcagccag gcgacgatgg atgaacgtaa
     1681 ccgtcagatt gcggaaattg gcgcgagcct gattaaacat tggtaaaccg atacaattaa
     1741 aggctccttt tggagccttt ttttttggac gacccttgtc agatcaaagg atcttc
//
