LOCUS       sequence-464700-        2371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            CDS_mRFP_PartType6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK074 [CDS_mRFP_PartType6]
FEATURES             Location/Qualifiers
     source          1..2371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             617..1294
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        1349..1440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1441..1509,1510..2301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2305..2344
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctcttatgg cgagtagcga agacgttatc aaagagttca tgcgtttcaa
      661 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
      721 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
      781 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
      841 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
      901 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
      961 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
     1021 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
     1081 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
     1141 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
     1201 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     1261 acgtgctgaa ggtcgtcact ccaccggtgc ttaaatcctg agaccagagt agcccgccta
     1321 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
     1381 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1441 atgagcattc agcattttcg tgtggcgctg attccgtttt ttgcggcgtt ttgcctgccg
     1501 gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg cggaagatca actgggtgcg
     1561 cgcgtgggct atattgaact ggatctgaac agcggcaaaa ttctggaatc ttttcgtccg
     1621 gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc tgtgcggtgc ggttctgagc
     1681 cgtgtggatg cgggccagga acaactgggc cgtcgtattc attatagcca gaacgatctg
     1741 gtggaatata gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg
     1801 tgcagcgcgg cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt
     1861 ggcggtccga aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg
     1921 gatcgttggg aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg
     1981 ccggcagcaa tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca
     2041 agccgccagc aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt
     2101 agcgcgctgc cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc
     2161 cgtggcatta ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat
     2221 accaccggca gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg
     2281 agcctgatta aacattggta aaccgataca attaaaggct ccttttggag cctttttttt
     2341 tggacgaccc ttgtcagatc aaaggatctt c
//
