LOCUS       sequence-464711-        2410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            CDS_sfGFP_PartType9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK085 [CDS_sfGFP_PartType9]
FEATURES             Location/Qualifiers
     source          1..2410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             617..1330
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        1388..1479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1480..1548,1549..2340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2344..2383
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaatgc gtaaaggcga agagctgttc actggtgtcg tccctattct
      661 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
      721 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
      781 tccttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
      841 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
      901 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
      961 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aggacggcaa
     1021 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
     1081 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
     1141 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
     1201 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
     1261 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
     1321 actgtacaaa tgaatcttga gaccagagta gcccgcctaa tgagcgggct tttttttaat
     1381 tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1441 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt
     1501 gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc
     1561 ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg
     1621 gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg
     1681 agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa
     1741 caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc
     1801 gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg
     1861 agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc
     1921 gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg
     1981 aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc
     2041 ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat
     2101 tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg
     2161 tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg
     2221 ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg
     2281 atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa
     2341 accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtcagatca
     2401 aaggatcttc
//
