LOCUS       sequence-464714-        2410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            CDS_GFP_PartType9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK088 [CDS_GFP_PartType9]
FEATURES             Location/Qualifiers
     source          1..2410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             617..1330
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(647..675)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1270..1290
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        1388..1479
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1480..1548,1549..2340)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2344..2383
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctaatga gtaaaggaga agaacttttc actggagttg tcccaattct
      661 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
      721 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
      781 tccatggcca acacttgtca ctactttgac ttatggtgtt caatgctttt caagataccc
      841 agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
      901 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
      961 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1021 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1081 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     1141 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     1201 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     1261 ggaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     1321 actatacaaa tagatcttga gaccagagta gcccgcctaa tgagcgggct tttttttaat
     1381 tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     1441 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca gcattttcgt
     1501 gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca tccggaaacc
     1561 ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta tattgaactg
     1621 gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt tccgatgatg
     1681 agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc gggccaggaa
     1741 caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatatag cccggtgacc
     1801 gaaaaacatc tgaccgatgg catgaccgtg cgtgaactgt gcagcgcggc gattaccatg
     1861 agcgataaca ccgcggcgaa cctgctgctg acgaccattg gcggtccgaa agaactgacc
     1921 gcgtttctgc ataacatggg cgatcatgtg acccgtctgg atcgttggga accggaactg
     1981 aacgaagcga ttccgaacga tgaacgtgat accaccatgc cggcagcaat ggcgaccacc
     2041 ctgcgtaaac tgctgacggg tgagctgctg accctggcaa gccgccagca actgattgat
     2101 tggatggaag cggataaagt ggcgggtccg ctgctgcgta gcgcgctgcc ggctggctgg
     2161 tttattgcgg ataaaagcgg tgcgggcgaa cgtggcagcc gtggcattat tgcggcgctg
     2221 ggcccggatg gtaaaccgag ccgtattgtg gtgatttata ccaccggcag ccaggcgacg
     2281 atggatgaac gtaaccgtca gattgcggaa attggcgcga gcctgattaa acattggtaa
     2341 accgatacaa ttaaaggctc cttttggagc cttttttttt ggacgaccct tgtcagatca
     2401 aaggatcttc
//
