LOCUS       sequence-464720-        1768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  XanthoMoClo Golden Gate Part Plasmid for cloning, contains 
            Term_rrnB-T1_PartType7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Soltysiak MPM, Ory ALH, Lee AD, Christophersen CE, Jalihal AP, 
            Springer M
  TITLE     XanthoMoClo horizontal line A Robust Modular Cloning Genetic Toolkit
            for the Genera Xanthobacter and Roseixanthobacter.
  JOURNAL   ACS Synth Biol. 2025 Apr 18;14(4):1173-1190. doi: 
            10.1021/acssynbio.4c00806. Epub 2025 Mar 13.
  PUBMED    40080684
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00806"; journalName: "ACS Synth Biol"; date: 
            "2025-04-18- 18"; volume: "14"; issue: "4"; pages: "1173-1190"
COMMENT     Sequence Label: pXTK094 [Term_rrnB-T1_PartType7]
FEATURES             Location/Qualifiers
     source          1..1768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      620..691
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        746..837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(838..906,907..1698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      1702..1741
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcgagtcgg tctctatccc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      661 ttttatctgt tgtttgtcgg tgaacgctct cgctgtgaga ccagagtagc ccgcctaatg
      721 agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat
      781 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      841 agcattcagc attttcgtgt ggcgctgatt ccgttttttg cggcgttttg cctgccggtg
      901 tttgcgcatc cggaaaccct ggtgaaagtg aaagatgcgg aagatcaact gggtgcgcgc
      961 gtgggctata ttgaactgga tctgaacagc ggcaaaattc tggaatcttt tcgtccggaa
     1021 gaacgttttc cgatgatgag cacctttaaa gtgctgctgt gcggtgcggt tctgagccgt
     1081 gtggatgcgg gccaggaaca actgggccgt cgtattcatt atagccagaa cgatctggtg
     1141 gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg tgaactgtgc
     1201 agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac gaccattggc
     1261 ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac ccgtctggat
     1321 cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac caccatgccg
     1381 gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac cctggcaagc
     1441 cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct gctgcgtagc
     1501 gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg tggcagccgt
     1561 ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt gatttatacc
     1621 accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc
     1681 ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg
     1741 acgacccttg tcagatcaaa ggatcttc
//
