LOCUS       sequence-464731-        7205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid of LetB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7205)
  AUTHORS   Santarossa CC, Li Y, Yousef S, Hasdemir HS, Rodriguez CC, Haase MB, 
            Baek M, Coudray N, Pavek JG, Focke KN, Silverberg AL, Bautista C, 
            Yeh J, Marty MT, Baker D, Tajkhorshid E, Ekiert DC, Bhabha G
  TITLE     LetA defines a structurally distinct transporter family involved in 
            lipid trafficking
  JOURNAL   bioRxiv 2025.03.21.644421
REFERENCE   2  (bases 1 to 7205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.21.644421"
COMMENT     Sequence Label: pBEL2886
FEATURES             Location/Qualifiers
     source          1..7205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..294
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     217..236
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(3073..3090)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3243..3329
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3421..3448
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3467..3558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3559..3627,3628..4419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3777..3796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4461..4916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4548..4567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4758..4779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5027..5614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5515..5534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5768..5785
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     5951..5973
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6111..6130
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(6310..7188)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt
       61 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa
      121 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag
      181 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa
      241 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt
      301 ttttttgggc tagccgaagg gtgaattatg agtcaggaaa cgcccgcttc gacgactgaa
      361 gcgcagatta aaaataaacg ccgtatctca cctttctggc tgctgccgtt catcgcgcta
      421 atgattgcca gttggctgat ttgggacagt tatcaggacc ggggtaatac cgtcaccatc
      481 gactttatgt cggcggatgg tattgttcca ggccgtacgc ctgttcgtta tcagggcgtt
      541 gaagtcggaa cagtgcagga tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag
      601 gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag agactcagtt ctggctggtg
      661 acgccaaaag catcgttggc aggtgtctcc gggctggacg ccctcgtcgg tgggaactat
      721 atcggcatga tgccgggtaa aggtaaagag caggatcact ttgtcgcact cgatacccaa
      781 ccgaaatatc ggctggacaa tggcgatctg atgatccacc tgcaagcccc cgatctcggt
      841 tcgctgaaca gcggttcatt ggtctatttc cgcaagatcc cggtgggaaa agtctacgac
      901 tatgccatca atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt
      961 accgatctgg tgaaaaaagg tagccgtttc tggaacgttt ccggcgttga tgccaacgtc
     1021 agtatcagtg gcgcgaaggt gaaactggaa agcctggcgg cactggttaa cggtgcgatt
     1081 gccttcgatt caccagaaga gtcgaaacct gccgaggcgg aagatacctt tggtctgtat
     1141 gaagatctgg cccacagcca gcgtggcgta ataataaaac tggaactgcc gagtggggcc
     1201 ggattaaccg ccgactcgac gccgttaatg tatcaggggc tggaagtcgg acagctgact
     1261 aaactggatt taaatcctgg tggtaaagtc acaggggaaa tgaccgttga tcccagcgtc
     1321 gttaccctgc ttcgtgaaaa tacccgcatc gaattacgca acccgaaatt atcccttagc
     1381 gatgctaatc tcagcgccct gctgaccggc aaaaccttcg agctggtgcc cggcgatggc
     1441 gagccacgca aagagttcgt tgttgtgcca ggcgaaaaag cactgctgca tgaacctgat
     1501 gttctgacgc tgaccctgac cgcgccggaa agttacggta ttgatgcggg tcagccgctc
     1561 attcttcacg gcgtgcaggt aggccaggtt atcgatcgta aactcaccag caaaggcgtc
     1621 acctttaccg tcgccatcga gcctcagcat cgcgaactgg taaaaggcga tagcaaattt
     1681 gtcgtcaaca gccgtgtcga tgtgaaggtg gggctggatg gcgttgagtt tctcggtgcc
     1741 agcgcctcag aatggatcaa tggcgggata cgtattctgc cgggcgataa aggtgagatg
     1801 aaagccagct atccactgta tgccaatttg gaaaaagcgc tggagaacag ccttagcgat
     1861 ttacccacca caaccgtgag tttgagtgca gagacgctgc cggatgtgca ggcaggatcg
     1921 gtagtgctgt accgtaaatt tgaagttggt gaagtgatta ccgtgcgtcc gcgagctaac
     1981 gcgtttgata tcgatctgca tattaagccg gagtatcgca accttctgac cagcaatagc
     2041 gtgttctggg cagaaggcgg ggcgaaagtt cagctgaatg gtagtggtct gaccgtacag
     2101 gcatccccgc tctccagagc attaaaggga gccattagct tcgacaacct cagcggtgcc
     2161 agcgccagtc agcgtaaagg cgataagcgt attctgtatg cttccgaaac agcggcccgt
     2221 gcggttggcg ggcagattac gcttcacgct ttcgatgccg gaaaactggc ggtcgggatg
     2281 ccaattcgct atctcggtat tgatatcggg caaatccaga cgctggatct gattaccgca
     2341 cgcaatgaag tacaggcaaa ggcggtactt tacccggaat atgtccagac ctttgctcgc
     2401 ggtggtacgc gcttctcagt ggtcacaccg caaatttcgg cagctggcgt tgagcatctt
     2461 gatactatcc tccagccgta tatcaacgtc gaaccaggcc ggggcaatcc tcgccgcgac
     2521 tttgaattac aagaggccac cattactgat tcgcgttacc tggatggctt aagcattatt
     2581 gttgaagcgc cggaagccgg ttcgttaggc atcggtacgc ctgtgctgtt ccgtggtctg
     2641 gaagtcggta cggttacagg aatgacgctg gggacattgt cagatcgcgt gatgattgcg
     2701 atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtctt ctggttggca
     2761 tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg cacctttaac
     2821 cagtttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact ggcaccgaaa
     2881 gcccaggaag gcaaacactt cctgttgcag gaaagtgaac cgaaagagtg gcgtgaatgg
     2941 ggaactgcgc ttcccaaata aggtaccaaa ttccagaaaa gagacgctga aaagcgtctt
     3001 ttttcgtttt ggtcctggtc tgaggaatgg ttgtaagctt ggctgttttg gcggatgaga
     3061 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     3121 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     3181 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     3241 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     3301 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     3361 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     3421 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc
     3481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3781 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     3841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4081 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     4141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4441 tatatacttt agattgattt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4501 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4561 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4621 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     4681 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4741 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4801 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4861 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttaaaa
     4921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5101 tgccggatca agagctacca actctttttc cgaaggtaac tggctcagca gagcgcagat
     5161 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     5701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5761 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     5821 tacgcatctg tgcggtattt cacaccgcat agggtcatgg ctgcgccccg acacccgcca
     5881 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5941 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     6001 aggcagaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     6061 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     6121 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     6181 gcgtagagga tctgctcatg tttgacagct tatcatcgat gcataatgtg cctgtcaaat
     6241 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt
     6301 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
     6361 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
     6421 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
     6481 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
     6541 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
     6601 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
     6661 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
     6721 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
     6781 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
     6841 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
     6901 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
     6961 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
     7021 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
     7081 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
     7141 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
     7201 cccgc
//
