LOCUS       sequence-464737-        7022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetic complementation plasmid of LetA(Delta-2-17) with LetB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Santarossa CC, Li Y, Yousef S, Hasdemir HS, Rodriguez CC, Haase MB, 
            Baek M, Coudray N, Pavek JG, Focke KN, Silverberg AL, Bautista C, 
            Yeh J, Marty MT, Baker D, Tajkhorshid E, Ekiert DC, Bhabha G
  TITLE     LetA defines a structurally distinct transporter family involved in 
            lipid trafficking
  JOURNAL   bioRxiv 2025.03.21.644421
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.21.644421"
COMMENT     Sequence Label: pBEL2635
FEATURES             Location/Qualifiers
     source          1..7022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(4041..4059)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4055..4102
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4147..4165)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4233..4337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4338..4406,4407..5198)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4556..4575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5369..5957
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5858..5877
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6111..6128
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6143..6282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6208..6227)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6368..6390
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6384..6575)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatgatcggc gaggaactgc cgcgtggtga ttaccaacgt
      181 tgcccgcaat gtgacatgct gtttagcctg cccgagataa attctcatca aagtgcctat
      241 tgtccgcgct gtcaggcaaa aattcgtgac gggcgcgact ggtcgctaac gcgcctggcg
      301 gcaatggcct tcaccatgct gttgcttatg ccgtttgcct ggggcgaacc gctgttgcat
      361 atctggctgt taggcattcg tattgacgcc aacgttatgc aaggcatctg gcaaatgacc
      421 aaacagggcg atgcgataac ggggtcgatg gtctttttct gcgttattgg tgccccgctc
      481 attctggtga cctccatagc ttatttatgg tttggtaacc gactgggaat gaatttacgt
      541 ccggtactgc tgatgcttga gcgacttaaa gagtgggtaa tgctcgatat ctacctggtc
      601 ggcattggcg ttgcttctat aaaggtacag gattatgccc atatccaggc tggtgtcggc
      661 ttgttctctt ttgtggcgtt ggtgatttta acgacggtga cgttgtcaca tcttaatgtc
      721 gaagaactgt gggagcgatt ttatccgcag cgccccgcta cgcgtaggga cgagaaactt
      781 cgtgtctgcc tagggtgcca ttttaccggc tatccagatc agcgtggtcg ctgcccgcgt
      841 tgccatatcc cgctacgcct gcgtcgccgt catagtctgc aaaaatgctg ggcggcgctg
      901 ttagcgtcaa tcgttttatt gttacctgcc aacctgttgc ctatttctat catttatctg
      961 aacggaggac ggcaggaaga tacaattctt tccggaatta tgtcgctggc aagtagcaac
     1021 attgcggttg caggaatcgt gtttatcgcc agtattctgg tcccgtttac taaagtgatc
     1081 gtcatgttca ctttactgtt gagcattcat tttaaatgcc agcaaggttt acgcacacgc
     1141 attctgttac tgcggatggt gacctggatt ggtcgctggt cgatgctcga cctgtttgtc
     1201 atatctttaa ccatgtcgct gattaatcgc gatcagatcc tcgcttttac tatgggaccg
     1261 gctgcgtttt atttcggcgc agcggtaatt ttgactattc ttgctgtgga atggctggac
     1321 agccgcttac tttgggatgc acatgagtca ggaaacgccc gcttcgacga ctgaagcgca
     1381 gattaaaaat aaacgccgta tctcaccttt ctggctgctg ccgttcatcg cgctaatgat
     1441 tgccagttgg ctgatttggg acagttatca ggaccggggt aataccgtca ccatcgactt
     1501 tatgtcggcg gatggtattg ttccaggccg tacgcctgtt cgttatcagg gcgttgaagt
     1561 cggaacagtg caggacatca gcctcagcga cgatcttcgt aagattgaag tcaaggtcag
     1621 catcaagtcc gatatgaaag atgcgctgcg cgaagagact cagttctggc tggtgacgcc
     1681 aaaagcatcg ttggcaggtg tctccgggct ggacgccctc gtcggtggga actatatcgg
     1741 catgatgccc gggaaaggta aagagcagga tcactttgtc gcactcgata cccaaccgaa
     1801 atatcggctg gacaatggcg atctgatgat ccacctgcaa gcccccgatc tcggttcgct
     1861 gaacagcggt tcattggtct atttccgcaa gatcccggtg ggaaaagtct acgactatgc
     1921 catcaatccc aacaagcaag gcgtggtgat tgatgtcctg atcgagcggc gttttaccga
     1981 tctggtgaaa aaaggtagcc gtttctggaa cgtttccggc gttgatgcca acgtcagtat
     2041 cagtggcgcg aaggtgaaac tggaaagctt ggcggcactg gttaacggtg cgattgcctt
     2101 cgattcacca gaagagtcga aacctgccga ggcggaagat acctttggtc tgtatgaaga
     2161 tctggcccac agccagcgtg gcgtaataat aaaactggaa ctgccgagtg gggccggatt
     2221 aaccgccgac tcgacgccgt taatgtatca ggggctggaa gtcggacagc tgactaaact
     2281 ggatttaaat cctggtggta aagtcacagg ggaaatgacc gttgatccca gcgtcgttac
     2341 cctgcttcgt gaaaataccc gcatcgaatt acgcaacccg aaattatccc ttagcgatgc
     2401 taatctcagc gccctgctga ccggcaaaac cttcgagctg gtgcccggcg atggcgagcc
     2461 acgcaaagaa ttcgttgttg tgccaggcga aaaagcactg ctgcatgaac ctgatgttct
     2521 gacgctgacc ctgaccgcgc cggaaagtta cggtattgat gcgggtcagc cgctcattct
     2581 tcacggcgtg caggtaggcc aggttatcga tcgtaaactc accagcaaag gcgtcacctt
     2641 taccgtcgcc atcgagcctc agcatcgcga actggtaaaa ggcgatagca aatttgtcgt
     2701 caacagccgt gtcgatgtga aggtggggct ggatggcgtt gagtttctcg gtgccagcgc
     2761 ctcagaatgg atcaatggcg ggatccgtat tctgccgggc gataaaggtg agatgaaagc
     2821 cagctatcca ctgtatgcca atttggaaaa agcgctggag aacagcctta gcgatttacc
     2881 caccacaacc gtgagtttga gtgcagagac gctgccggat gtgcaggcag gatcggtagt
     2941 gctgtaccgt aaatttgaag ttggtgaagt gattaccgtg cgtccgcgag ctaacgcgtt
     3001 tgacatcgat ctgcatatta agccggagta tcgcaacctt ctgaccagca atagcgtgtt
     3061 ctgggcagaa ggcggggcga aagttcagct gaatggtagt ggtctgaccg tacaggcatc
     3121 cccgctctcc agagctctaa agggagccat tagcttcgac aacctcagcg gtgccagcgc
     3181 cagtcagcgt aaaggcgata agcgtattct gtatgcttcc gaaacagcgg cccgtgcggt
     3241 tggcgggcag attacgcttc acgctttcga tgccggaaaa ctggcggtcg ggatgccaat
     3301 tcgctatctc ggtattgaca tcgggcaaat ccagacgctg gatctgatta ccgcacgcaa
     3361 tgaagtacag gcaaaggcgg tactttaccc ggaatatgtc cagacctttg ctcgcggtgg
     3421 tacgcgcttc tcagtggtca caccgcaaat ttcggcagct ggcgttgagc atcttgatac
     3481 tatcctccag ccgtatatca acgtcgaacc aggccggggc aatcctcgcc gcgactttga
     3541 attacaagag gccaccatta ctgattcgcg gtacctggat ggcttaagca ttattgttga
     3601 agcgccggaa gccggttcgt taggcatcgg tacgcctgtg ctgttccgtg gtctggaagt
     3661 cggtacggtt acaggaatga cgctggggac attgtcagat cgcgtgatga ttgcgatgcg
     3721 catcagtaaa cgctatcaac acctggtgcg taacaattcc gtcttctggt tggcatcggg
     3781 ttacagtctg gactttggtc tgacgggcgg cgtagtgaaa accggcacct ttaaccagtt
     3841 tatccgtggc ggcatcgcct tcgccacgcc tccgggtacg ccactggcac cgaaagccca
     3901 ggaaggcaaa cacttcctgt tgcaggaaag tgaaccgaaa gagtggcgtg aatggggaac
     3961 tgcgcttccc aaataatgcc cacctcgagc agatccggct gctaacaaag cccgaaagga
     4021 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     4081 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggataat tcttgaagac
     4141 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4201 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4261 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4321 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4381 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4441 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4501 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4561 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     4621 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4681 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4741 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4801 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4861 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4921 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4981 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5041 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5101 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5161 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5221 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5281 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5401 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5461 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     5521 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5581 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5641 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5701 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5761 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5821 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5881 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5941 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6001 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6061 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6121 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6181 tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6241 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     6301 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6361 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6421 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     6481 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     6541 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     6601 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     6661 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     6721 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     6781 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     6841 ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     6901 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     6961 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     7021 ag
//
