LOCUS       sequence-464743-       20441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0469) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20441)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.1-Dadh-Delta-Elen0469(ElA2)
FEATURES             Location/Qualifiers
     source          1..20441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(2136..2159)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14065..14092
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14224..14251
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14683..15534)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15521..16360)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16871..17842)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19081..19168
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19510..19904
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20067..20110)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20206..20315
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttac
     1501 tcatcacctc cttcccagtg atggtcgcgt aggatgtagg agtacgacgg cttgttgccc
     1561 acgtcgggca gcttgtgcac gtcggcgtcg gtgaacggct ccaggaactc caccatcttg
     1621 cggcgcttgc ccagcgggtc ctcggagccc tcgaacgact cgtagccggc ctcggtgtcg
     1681 ccgatccagc gggcgttgcc gccgcactgg ctgacgcact ggggcagctc gccctgcgac
     1741 agcttctgct cgcacagcgt gcacttctcg acgaccttct cctcctcgtt gaggtagcgg
     1801 acgccgtagg ggcaggccat gacgcagaac tggcagccga tgcacttctc cttgtcgatc
     1861 tggatggagc cgtcctcggc cacgtgcgac gcctcggtgg ggcacacctt cacgcactcg
     1921 gggttctcgc agtgctggca gctgatgggc aggaagtaca tgtacacgtc ggggtactgc
     1981 cctgatccgc ccgggatggg gttcggcccc acgcgcacca ctttgttcca gtagctgccg
     2041 atgggcacgt tgttgaccgc cttgcaggcg accgagcacg ccaggcagcc cacgcaccgg
     2101 ttgaggtcgg tgatgatagc cttcgtcatg gttcagtgat ggtgatggtg atggtgatgc
     2161 tcgagtgcgg cctccctccc ttcgtaggta ggcagccatt ccttcagacg ggggtcgtcg
     2221 gaggtatgga tgatgggcgt gccggtggag tcgcacggca cggggttgtt gaacggcgag
     2281 ttctcgggcg tggccttgta gatcttcacc tggtaggcgc gcaggttgga cgtgccggag
     2341 tgcgggtcct gggcgtagtg gtcgatcagc tggttcacct gggagaactg ccagccgtgg
     2401 ccggcgtcct tcacctcggg gtaccaccac gtgtgctcga ggttgatgac gtcttccttc
     2461 acgccgtagt acaggtcagc cacttcgcgg atcttgcccc actcggtttc gatccacacc
     2521 cagtcgccct gctcgatgcc gtactcggcg gccgtcttcg ggttgatctc cacgcgcggc
     2581 acgggccaca gctcgcggca ccagggaagc tggcgatgct cggagtggaa gtacaccggg
     2641 atgcgacggc cggtggtggc ggtcagcggg tactccttga tgcggtcgcc gtccttcggg
     2701 ccgtgaggcg gctcggtgta ggtgggaagc ctccactcgg gatggtcggg atggtgcgat
     2761 tccatgacgg tggaccagat ctcctgcttc atcgtcgggg tgaaccagcc cggcttccag
     2821 tcgccgatgc ccttgccggc cgtgtagtcg aggcggcccc acacgcggtc gcgtgcgcgc
     2881 atcgcgccgg tctggtagcg gcggtacgtg ccccagttgc gcggctcgat gtccttcgcc
     2941 tgccaccagc cgtgctcctg gaaggccttg acgtactcgt cccacgtctt gtacttgggc
     3001 gtgacctcgg ccatcggaac gccgtggcgc tcgaacgtgg gcttgccgtt ctcgacgtgc
     3061 cacgtggtgt aggtcagctc gtcgtcggtg agcagcttga tggagtcgtc cagctgccag
     3121 ttgatgtcgg gccactcgtt gcccggctcg tcggtccact tccagttcat gcggcgggcg
     3181 aggtccatga cgatctcggg atcgtacttg gcttccgcgg gcggctgcac gcacttgacc
     3241 gtggcgccca tggcgccggc ggaaccttgg ctggcgcgcg gcgagttgag ctcgatccaa
     3301 tgggccaccg gcaggatgat gtcggcggtg tcgttctgcg gcgtgtgcca caggttgagg
     3361 tccacgtaga agtcaagcga gcacagggcc tcccaggcgc gcgtggtgtt cgtctggctc
     3421 atgaagttgc cggactcgtt ccagagggcg cgcaccgggt aggggtcgcc cgtgatgacg
     3481 gcgtcccaca gcgaatggct gtcgcaccag tactgccacc agcccagaag cgggaacttg
     3541 tcgatgccga tctgcttgcg gttgacttcc tccggcgggg tggtggcgcc gggggcccag
     3601 gcgctgaagc cctggaggtc gccgtcgatg gggacgatgg tcgggccgcg gttgccgccc
     3661 ggggtgtcca tgttgccggt gatgcccacg aggttgtcga aggcgcggca gttctggatg
     3721 gcgctgcaga agtgctcgac cgccagcatg tactggatgc cgccgttgcc gtagccggtg
     3781 gacgggtcga tgcgcgtgcc gtaggccgtg gccgccgcct cgatctcgga ggcggggatg
     3841 ccggtgatct cggccgccac ctcgggcttg tactcggcgg cgcgggcctt gtagtgctcc
     3901 cacaccggct tgaccttcac ggtcttgccg tccttcagcg tgatctcgaa ctcgccttcc
     3961 agcgcggggt cgatggcggg gtcgaacggc atgggatcgg gcagccagcc ctgcgtctgg
     4021 cccggcagca ggttgtcctg cgcggcctcg cggccttggc gcgccttctc ggagtcgtag
     4081 ttctcgccct cccagaagcc gccggtctcg tcgatgacgc cttcctggtc ggcgttgaac
     4141 caggtgaacg cgccgtactc gtgctcgacg ccctcggcct tcagcttctc ccagttgttg
     4201 tcgtacacga ggaacttata ggggctgccg ccctccttga tgtcgctttc cttgagcagc
     4261 gcggttttca cgtcccagta ggaaccgtcg gtgcgcacgg tggggaagcc ggagggctcc
     4321 atgtcctcgc acacgaggaa cggggcgttc gtccacttct tcacgtacag ctcgtcgtag
     4381 agcttcttct cgatgatgac gttcgtccag gccagggcca gggcgccgtc ggtgccgggg
     4441 cgcaggtgct gccagtagtc ggcttccttg cccatgttgg ccatgcgggg gtcgacgctg
     4501 atgtgcacgt cggcgcgcga tgccacgtcc accgtggtgc ggcaggagtc gtcgtagttg
     4561 gacagctcgg acgcgccgcc ccactgcacg tacactttcg ggcgcgtgat ggtttccatc
     4621 cacgacatgg cgaactgcga caccatggtg gtggcgaagt ggcgcgggcc cttgcagatc
     4681 tgccaggcct caacgttgtt cggcgtttcc agcatgttct tcaggatgga ctccgagtgc
     4741 atgcaccaga tgcgcgacgt gcccacctgg caggcgatgg cctcgccgcc gtgcttcgcc
     4801 ttgatgtcca tgaagttgtc cacgatggac tgcatggcct cgtcccacga gatgcgctgc
     4861 cagccgggct cctcgccctt cgggttcgtg cgcttcatgg gatagtgcag acggtcgggg
     4921 tggtacgccg cctggatgga cgactgcgac ttcgtgcagc agttgcccat ggactggaac
     4981 gctccctcgt cgccctcgac gcgtatggcc cgcccgttct ggacgatgac cttgacgccg
     5041 cactccatct tgccgcagcc gcggcagcac gttttcacgg ccacggtgtc gttgccggac
     5101 acgctgctgt acgtctcttc ggccagcgcg gtgtgcgtcg aagcgccgaa cgccgcggct
     5161 gccgcaccgg tgatggcggc ggtcttgacg aacgtgcgtc gtgacatggt caggttaccc
     5221 atactcctcc tccttctttc tcctcgttct cgtcttgctc gtcttcgttc gtgcgatcgg
     5281 ggaggggggc tgccgggtga agcctcgggc tccgaaccgg tgcaaagtcc ccgtgtcgct
     5341 tgggccgacc atacggatgc gagggggtgc agagcatcgt ccaaaggttg gaaagcaggt
     5401 tcgaccgcat cataaattct tatgatcttg tgatcatgaa aaattcgata gtgctttgaa
     5461 ctggggaaac tttaaccgta ggtcataaat tattctgaaa ctagaatagt ttcctcaaag
     5521 agggccgctc atcttttccg tataatcgaa cccgtcgaca gaggggtcgg cgcgagcgac
     5581 gttatgtacg ggctcacagg gggtggggca tggaaaagct tcggagtgca tgggataccg
     5641 cgttggggac gagcctttcc ctcaagcgca tcgcgggcgc ggccttgcaa tggctttgga
     5701 tgctcatgat gttctacagc atcgtgccgt acggcttctc gtcggacgtg cgcggctcgc
     5761 tgtacgtcag cctgatcata tcgctggtgg ccatggtggt gaccatgctg ttcgtctccg
     5821 tcatcgtgcg gcgcgagcgg gtgagcggca accgctccat cgtgctggga tcggccatca
     5881 tgatgagcgc gggcagcgtg ctcacgccgt tctccgatcc taccacgccc gtcggcatgc
     5941 tggtgctggg cctgagcgcg gtcatgaccg gcacgggctc ggccgtgctg ttcttgtgct
     6001 ggatcgagct cgagtcgggg ctgggaggcc gcttggcgct tgtggagctt gcctcgtcgc
     6061 tgtgcatcgc gttcgtcgtg gggttcttgc tgatcgtagg gccggcgata cccgtcatcg
     6121 tgctcatcgt tgtcatgccc gtgctttcgg cggcagctct caggcgcagc gcgcccgata
     6181 cgccgcagct gccgcgcgag cccgagcagc ctttgtcgcg ccatacggcg accctgttcg
     6241 cgaaggcgct tctgggcgcg gtgctcatcg gcatgttgat ggggttcttc gacgtggtgt
     6301 cggggttcaa aacctacgag gtgcaggaca tcttcggcac gtacctgttt ctggcgggct
     6361 tcatcggagc gttggccatc tgcctgatag ccgtgttcct gcagcgcgac agcgtgttct
     6421 tctcgtaccg cgtctccatg ctgatgctgt gcttgggatg cttgtccacg ccgttcatgg
     6481 ccgacaacaa cacgtattcg agcgcgctta tcttcggcgg ctaccactgc ttcgtgctgg
     6541 tgctgtgcgt ggtgtgcatc gacgtggcgt ccagcttccg cgtgagcccg gcgcggacga
     6601 tcggcctggg gttcgtggcg ctgtacggcg gcgagaccgc aggccagctg ttcgcccatt
     6661 cgctggaggc gaccggggtc agcatgttcg acctcacgct ggtcacgctg gtggccgtgt
     6721 cgctgctgtt catcgcgcat ttgttcctgt tcacggagac cgacctcatc aaaatcggca
     6781 tcggcgaggt cagcctgatg ggcacggtgc ccgaagaggc cgccgacgac ccggccgacc
     6841 cggtggatcc gtgcgcgctg atcgcggagc gcttcgccct gtcgccgcgc gagaccgacg
     6901 tgcttccgct gctgctggag ggccgcacca tctcgcgcat ccaggagacg ctgttcatct
     6961 cggcgggcac ggtgagcacg catatccgcc acatctacca gaagacgggc gtggacaatc
     7021 gccaggagct catcgacctg gcccacgaga cggccgcgca ggacgaggcg gtctgaggcg
     7081 atggagacgg cgtatctgca cgagttcgcg aaggtggccg attgcggcag cttcacggcc
     7141 gccgcgcgcg agctgcatct gacgcagtcg acgctgagca agcacgtggc cttgttggag
     7201 cgcgaattcg gggccgacct gttcgtgcgc gaccgcagcg gcgtgagcct gaccgaggcg
     7261 ggcggcgtgc tgtacgccca ggcgcggcag atggagaagc tgctgcgcgc caccgagctg
     7321 ctggtgcgcg cggcgcgcga cgggcagggt tcggcgggcg cgggggttgc gggcgcgacg
     7381 gcgggcgcag cggcgggcgg gggtgccgtc gacggctcgc ctgcggccgc gtgcgacacg
     7441 gcgcttcgct gcaagtgccg gctcgcggcc gagcgctgcg ggctcgaccg gcgcgagctg
     7501 ggcgcgctca tcctgtacgt ggaggagcgc ggcttcgagg cgatccaggg cgagctggcg
     7561 ctgacgcgcg acgaggtggc cgacgtgctg ggcagcgtgt accgcaagct gggcgtgggc
     7621 gacaagcagg aggcgctcga tttcatccat tccgtttcgg aatgatggta gacgcgcccg
     7681 cggcggttgg gcatactgcg tagaagtgaa agcgctatga agctgggggg cttcgagcaa
     7741 agggcttcat ccgaagaatg gatggagacc gaatatgagt gcttggactg ctgcgtccgt
     7801 cgaagacgcg gcgaacaatc ttcttgaaga actgaatagc ggcgcgatgc gcttgtgcgt
     7861 ggagcatgac gcgtccgacg acgttgcgcc ggcgttgctg cgcgcggctc gcgctcgcgt
     7921 cgaggcgggg tcgaacgtgc gcgtcgtgtg cgccgacgcc gttgcagctg cgcgctttcg
     7981 ggcgcttgcg gccgacgatc cgctgctggg cgcgctcgac gtggtatcgg tgcgcgagct
     8041 ggccctctcg attctaggcg acgcgcgggt cggcgatgcc gtgggacgcg acgcgcgcgt
     8101 gctggacgag aacgagctgg ccgtgctcat ggaggacgtg aaggtgagcg gcctcaagcc
     8161 gggccggctg cgcgagatgc tcaagttctt ctacaagagc atcagcgact gcgccgacga
     8221 tgacgagcgc tggctgatca ccgccgaaga gcagaccgtg cacgccatcc tcaccgagaa
     8281 cctggaggcg cgccgcgcgt tgctgccgtg cgaggtttcg tcgctggcgt atcgcggcct
     8341 tgtgaaagcc ggggtcgagc gcgagccgct gacgctggtg gcagacgact tcgggtcgct
     8401 cagcaaggcg tcgcagcgcc tcgtgcgcca tctggcgacg gacggcctcg tggcggcggg
     8461 ccgcacgctg gcctcgtcca gctccgagga gccgtatccg tgcttcgacg ggttccgcgc
     8521 gcttgccgac gaggccgact gcctggtgac gctggcgtgc gagcggccgg ccgcggcgcg
     8581 cgagtcccgc gcgctggacg acccggcggc cgagttcgcg ttcgcggcgg cgtccgtgga
     8641 ggagtgcctg gccgacggct tccggccagg cgacgtggct gtggccgtgc cgaacgccgc
     8701 ttggggcgag cgcatcgccg ccgagctgga agggcgcggc atcgccgtgg agcgcgggtt
     8761 cgacagcggg aagatcaagg gcgacccgcg caccgagggc cgttacgccg acctcaagct
     8821 ggccgcgttc ctgcggttgt acctcgatcc gcacgatttc acgtcgttgc gctcgtggct
     8881 ggggttcggc gattggctgc tgcgctccga tgcgtttttg gagctgatgg cgtacgcgcg
     8941 cgaccatgag acgaccgtgc ccgaagccgt ggcgcagctg cgcacgcagc gcgacgccga
     9001 tcgctcctcg acgatattcg ggaagttcga cggcccgctc gacgagctgg acgagctgct
     9061 gcgcgcctgc gaggaagggc tgacgcgcga ggccgccgta gcgctgttcg aagcgcacgg
     9121 aatgccgttg tccccgcgcc acgtggagct gctgggcgac gaccctgcgc atgcggacgt
     9181 cgagcgcttg gcgcgctgcg ggttcgcgaa gccggtggag gatgtcgcgc gcgatgccgt
     9241 gcatgtggtc tcctacgcgc gctgccacgg gcaccatgtg cgcacggtgt tcgtcaccgg
     9301 cctggtgaac ggcttcttgc cggcgagcga cgccgtggac gacaagttca ccatcgacca
     9361 tcggcgcgtg gcgctcgaac gcgagcgcgc gctgttcctg gacgtgctgt cctgtgcgag
     9421 cgaacgagcg gtatgcacgc ggttcgtgcg cgacctttta gagaacaccg cgttcacgaa
     9481 ggtgcagacc tcgcgcgtgt tcgtgcgcga cggcgagcgc ttcgcgcgcg tgacgccgtc
     9541 ggagttcgcg tcgctgaccg acgaggtgct ctctcccgcg cccgacgcgc ctcgcgagct
     9601 gccgacgaag gtgctgtacg cgtcttcgac tttgtagctc caacggtctg acgaccgcgc
     9661 gaaccttcgc tgacttttcc tgaccggtga ttttgaaatg atgaaaggac tgatagatat
     9721 gagcgatgtt gatgtattcg tgatcgatga cgatttcgat cccctggccg acctgagcct
     9781 ggacgacggc gagcgggacg atgccgaggc ggattacctg ccgcccatcc ccgatgccga
     9841 caagagcgtg gtgcctcccg tggtgccgtt gtctgcgacc gagcgcatcg agaagctgct
     9901 ggcgggcatt cccggccagc agttccgcct gctgcacgcc gtggaattct gcaccgagcc
     9961 gaagacgatg gacgaggccg tggccaattt ggacgcggcg tatccgaaca cgacgagcgt
    10021 gtacggctcg gcgcaggtgg tgcagctgct cgagcgcgac ggcgcgctgg agcgcatcgt
    10081 ggacgaggac gccgcccatg cggccgccgg cgacgctgcc gatacgcccg ccgaggacga
    10141 gggcttcatc tcggtgacgc ccgctccgcc gtgtcactac cgtgccacgc aagcgggcct
    10201 cgatgccgtc gcggcgcacg tcaacgaggg cctcgtggcg gagcggatca gcgaagacga
    10261 gcgctatctg cccatcttcc agcgcgtgct tgagatgtgc gcgcgcgaag gaggctgccc
    10321 caccaaggag ctggaccagg cggtggacgg cgacccgctg tgccaagagc cgcgccgctt
    10381 ctgcgggttc ttccgcggca agctggaaga aacaggcgcc atcgaatggc gcgacgcctg
    10441 gaccattacc gatctggggc gcagcgtgct cgcgtccggc ttgttcgccg ccgcttcccc
    10501 gtccgaacgc tagagaagga gccacccatg tcccacgatc aggaaaccac catcgccgac
    10561 gtgctgcgcg gccgcatggc aacctaccag ttcctctcgc gcctgttccg cgtggaggtg
    10621 gaccaggagc tgtacgacac gctgatgtcc atgcgcttcc cggcgaacac cggcaacgcg
    10681 ctggtggacg aaggctaccg gatgatctgc ggttatctga gccaagccga cggcacggtg
    10741 ctcaccgagc tggcggtgga ctacgtgcgc gcgttcatcg ggtcgggcaa cgacggcttc
    10801 tcggccgcgt acccgtacga gtcggtgtac acgagcccga agcgcctcat gatgcaggac
    10861 gcccgcgacg aggtgctggt gctgtaccgc gccttcggtc tggacaagca ggaaagctgg
    10921 aaggagggcg aggaccatat cgcgctggag ctggagttcg agcagatcct atgcgagcgc
    10981 gctatcaggg cgtacgaggc cggcgacgag gacgagtgcc tgaagctgct gctgtcgcag
    11041 cgcaacttcc tggaggacca cctgctggcg tggtacccca tgatggcggc cgacttgcag
    11101 aagttcccgc agaccgactt ctacaagggc ctgggcaagc tgaccgacgg cttcctgcgc
    11161 aacgaccgcg aatttttgga cgccgtgctg tctgagaacg aagcggattg ccatccagag
    11221 cggaggccgc aggccgaagc cgaaggatcc cgtgcggcgt cggccgaaac cgaagtcgag
    11281 gttgcatgat ggacgagcac ggcatcgagc ccaagcgcga tacgggcccc tgcgtgtcgc
    11341 gccgcacgtt cgccctggga gccgtgggag cgtgcgccgt catcgggctg ggcggcgtga
    11401 agtacctgcc cagtgccacg ttgctgcgcc cgccgggcgg gcaggacgag gagcagctgg
    11461 cgcgcggctg cctgcattgc gagaagtgcc gcgaggtgtg cccgaagcat gccatcgctc
    11521 ctgctcatct tgaggacggc atcttgaacg cgcgcacacc gcgcatggac ttcaaaagcg
    11581 gctggtgcga tttctgcgag aacgagccgg gcgggccgaa gtgcgtcgcg gcgtgcccga
    11641 cgcatgcgct ttcgtgcccc gacccgtcgc aggcgatcat cggcaaggcg gagatcaacc
    11701 gcgactggtg ccttgccgcg aagggcatgg gctgccacga gtgcgtcgac gtgtgcaact
    11761 acgaggcgct cgagctgggc gccgacaacg tgccggtggt ggacgtggat gcgtgcaacg
    11821 gctgcggcgc ctgcgagctg gcgtgcatct cgctgtcggc aggttccatc acggccggcg
    11881 ccaccgatcg cgccatcgtc gtccgtccga ccgaagaagt ggaggcgtga tcaggatgaa
    11941 gaagctcaaa acctatcggc tgcggttcct gatcatgctg gcgctgctgg ccgtggtggc
    12001 cgtggggtac ttcacggccg gcggcatcgg caacttctgc ggcatcggct tcgagtccat
    12061 cacgctgctg tgccccctag gcgcgctgct ggccatgatc gccgagcgca cagccatccc
    12121 gatggccgtg atcagcgttg cggtcgtgct gctggtgtgt atcgtgctag gcaaggtgtt
    12181 ctgcgcctgg gcgtgccccg tgcacttcct gtcgcgagga aggaagaagg gggataaggg
    12241 ggacaaggag ggtaaagggg gcaaggttgc ttccccttgc tccgcgtgcg cttccccttg
    12301 cggcaaagcc aagggcatca agatcgacag ccgtcacggc atcctcgctg cggcgttggg
    12361 ctccacgctc gtcttcggct tcccggtgtt ctgcctcgtc tgcccgatcg gcctcacgtt
    12421 cgccacggtg ctgctggtca tgcgcctgtt cgcgttcggc gacgccacgt ggacggtcgt
    12481 cgccttcccg gccatcatcg cgctggagat cctgctgctg ccgaagtggt gccagcgctt
    12541 ctgcccgttg ggcgcgttgc tttcgctgtt cagcgggctg aaccggacgt tccgccccca
    12601 ggtggacgcc gagaagtgcc tgcgcgaggg tcgcggcaag gcctgcaacc tgtgcgagca
    12661 ggcatgcccc gagggcatca acctgcacga tgtcgcggcg ggcgagacca cgctcaacga
    12721 ctgcagcaag tgccgcgcgt gcgccgacgt gtgccccgag cgcgccatca cgttcccgtt
    12781 gctgccgaag aagcaggcgc aggcgacgcc gctgcccgtc gacgacgaga tcccggaaag
    12841 gggataggcc atgtccctga tcaacggcct gctggccctg ttgggcgaga tcgagagcga
    12901 tgccatcgcc gtgcatgagg aacgctgcat ctcggttcgc aaccgcaacg ccgactgcct
    12961 gcgctgcgtg gaggcatgca cgtcgggcgc gctggcgtac cgcgcgggcg agctgctcgt
    13021 agagcccgag cgctgcatcg gatgcggcac gtgcgccacg gcgtgcccca cctgcgccat
    13081 cgagctgcgc aacccgacgg atgccgagct gacggcgcag ctcaagcact ccatcgtggc
    13141 gacgaaaggc catccggtca tcgtgtgcga gcaggcgctc gaggcggcca acgtcgcggc
    13201 cgacgatgcg tcggccgcgt gcgcggtgcc gtgcctgggg cgcatggacg agtcggcgct
    13261 cgtggggttg gcggcgtacc gcgccttcga cgcgacgctg gcctgcggtt cgtgcgagac
    13321 gtgcccacat gctccgggcg gcgcgctcgt gcgcgaggtg gtggagggcg cgcgcaacct
    13381 gcttgaggcg ttcggatcct ccatgcccat cgacctggag gaatgcgtgc ccgagcgcgt
    13441 gttggagccc ggcgggtcgc acggccaggc aggcagcgac ggggtggggc ggcgcgactt
    13501 cttccgctcg gccaaggacg catcgacccg cgcagctggc gcggcggtgg ccgaggagtt
    13561 ggggatggtc gaggccgagc ccgttcctgc ggcgcatcgc aaagtgggcg cagatgggac
    13621 gctgtcgcag ttcgtcccca cgcgccgcgt gcggctgtac aactacctgc agcacgtggg
    13681 gcaaccgacg gccgacgagg tggagacgcg tgtcatcggc gccgtgtcca tcgatgccca
    13741 gaagtgcagt tcgtgccgca tgtgcgcggt gttctgtccc acgggcgcca tcaagaagct
    13801 cgacgagggc ggcgtgttcg gcatcgtgca ccgtccgagc gcctgcatgc agtgccgcct
    13861 gtgcgagcac atctgtcccg agcaggccat cgcggtgagc gggcgcgtgc ccatcaagca
    13921 gttcatgggc aaggaagccg tgtgcttcgc tatgaagcgt ccaacctgga ccccgaacaa
    13981 gccctcatcg atgtacgata agctgcacgc gacgctcggc gaacacttgg agatgtgcat
    14041 gttctagact agtcgactca cactggctca ccttcgggtg ggcctttctg cgtttatata
    14101 ctagagagag aatataaaaa gccagattat taatccggct tttttattat ttaattcctg
    14161 ttataaaaaa aggatcaatt ttgaactctc tcccaaagtt gatcccttaa cgatccctgc
    14221 aggagaaggc catcctgacg gatggccttt ttgaagcttt ctagaatcgg gggcccccgg
    14281 ggggattcta gaacaatgga agtcctcgtc tcgccctcga attagcccgc ttaatgagcg
    14341 ggcttttttt gaattaattc tcgcgagctg gcacgacagg tttcccgact ggaaagcggg
    14401 cagtgagcgc aacgcaatta atgtaagtta gcgcgaattg caagctggcc gacgcgctgg
    14461 gctacgtctt gctggcgttc gggagcagaa gagcatacat ctggaagcaa agccaggaaa
    14521 gcggcctatg gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc
    14581 ttttctgagc atggtatttt tcatggtatt accaattagc aggaaaataa gccattgaat
    14641 ataaaagata aaaatgtctt gtttacaata gagtgggggg ggtcagcctg ccgccttggg
    14701 ccgggtgatg tcgtacttgc ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag
    14761 ctccggcaac gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac cactggcctc
    14821 tgacggccag acatagccgc acaaggtatc tatggaagcc ttgccggttt tgccggggtc
    14881 gatccagcca cacagccgct ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac
    14941 ctcgtccatg ctgatgcgca catgctggcc gccacccatg acggcctgcg cgatcaaggg
    15001 gttcagggcc acgtacaggc gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg
    15061 aaacccctgc cgcttgcggc cattctgggc gatgatggat accttccaaa ggcgctcgat
    15121 gcagtcctgt atgtgcttga gcgccccacc actatcgacc tctgccccga tttcctttgc
    15181 cagcgcccga tagctacctt tgaccaccat ggcatcagcg gtgacggcct cccacttggg
    15241 ttccaggaac agccggagct gccgtccgcc ttcggtcttg ggttccgggc caagcactag
    15301 gccattaggc ccagccatgg ccaccagccc ttgcaggatg cgcagatcat cagcgcccag
    15361 cggctccggg ccgctgaact cgatccgctt gccgtcgccg tagtcatacg tcacgtccag
    15421 cttgctgcgc ttgcgctcgc cccgcttgag ggcacggaac aggccggggg ccagacagtg
    15481 cgccgggtcg tgtcggacgt ggctgaggct gtgcttgttc ttaggcttca ccacggggca
    15541 cccccttgct cttgcgctgc ctctccagca cggcgggctt gagcaccccg ccgtcatgcc
    15601 gcctgaacca ccgatcagcg aacggtgcgc catagttggc cttgctcaca ccgaagcgga
    15661 cgaagaaccg gcgctggtcg tcgtccacac cccattcctc ggcctcggcg ctggtcatgc
    15721 tcgacaggta ggactgccag cggatgttat cgaccagtac cgagctgccc cggctggcct
    15781 gctgctggtc gcctgcgccc atcatggccg cgcccttgct ggcatggtgc aggaacacga
    15841 tagagcaccc ggtatcggcg gcgatggcct ccatgcgacc gatgacctgg gccatggggc
    15901 cgctggcgtt ttcttcctcg atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc
    15961 ggccctcggc ggcgcgcttg aggccgtcga accactccgg ggccatgatg ttgggcaggc
    16021 tgccgatcag cggctggatc agcaggccgt cagccacggc ttgccgttcc tcggcgctga
    16081 ggtgcgcccc aagggcgtgc aggcggtgat gaatggcggt gggcgggtct tcggcgggca
    16141 ggtagatcac cgggccggtg ggcagttcgc ccacctccag cagatccggc ccgcctgcaa
    16201 tctgtgcggc cagttgcagg gccagcatgg atttaccggc accaccgggc gacaccagcg
    16261 ccccgaccgt accggccacc atgttgggca aaacgtagtc cagcggtggc ggcgctgctg
    16321 cgaacgcctc cagaatattg ataggcttat gggtagccat tgattgcctc ctttgcaggc
    16381 agttggtggt taggcgctgg cggggtcact acccccgccc tgcgccgctc tgagttcttc
    16441 caggcactcg cgcagcgcct cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat
    16501 ccctttggcc ttcatgcgct cggcatatcg cgcttggcgt acagcgtcag ggctggccag
    16561 caggtcgccg gtctgcttgt ccttttggtc tttcatatca gtcaccgaga aacttgccgg
    16621 ggccgaaagg cttgtcttcg cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg
    16681 ccatatcagc gactgaaaag cggccagcct cggccttgtt tgacgtataa ccaaagccac
    16741 cgggcaacca atagcccttg tcacttttga tcaggtagac cgaccctgaa gcgctttttt
    16801 cgtattccat aaaaccccct tctgtgcgtg agtactcata gtataacagg cgtgagtacc
    16861 aacgcaagca ctacatgctg aaatctggcc cgcccctgtc catgcctcgc tggcggggtg
    16921 ccggtgcccg tgccagctcg gcccgcgcaa gctggacgct gggcagaccc atgaccttgc
    16981 tgacggtgcg ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc
    17041 tggcctcggc catggccttg ccgatttcct cggcactgcg gccccggctg gccagcttct
    17101 gcgcggcgat aaagtcgcac ttgctgaggt catcaccgaa gcgcttgacc agcccggcca
    17161 tctcgctgcg gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc cgctcggaca
    17221 gttcgaggct ggccagcctg cgggccttct cctgctgccg ctgggcctgc tcgatctgct
    17281 ggccagcctg ctgcaccagc gccgggccag cggtggcggt cttgcccttg gattcacgca
    17341 gcagcaccca cggctgataa ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc
    17401 ccgccaagcg gccatagtgg cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg
    17461 ccagcgtccg ggcaatctgc ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc
    17521 atgcctgata gttcttcggg ctggtttcca ctaccagggc aggctcccgg ccctcggctt
    17581 tcatgtcatc caggtcaaac tcgctgaggt cgtccaccag caccagacca tgccgctcct
    17641 gctcggcggg cctgatatac acgtcattgc cctgggcatt catccgcttg agccatggcg
    17701 tgttctggag cacttcggcg gctgaccatt cccggttcat catctggccg gtggtggcgt
    17761 ccctgacgcc gatatcgaag cgctcacagc ccatggcctt gagctgtcgg cctatggcct
    17821 gcaaagtcct gtcgttcttc atcgggccac caagcgcagc cagatcgagc cgtcctcggt
    17881 tgtcagtggc gtcaggtcga gcaagagcaa cgatgcgatc agcagcacca ccgtaggcat
    17941 catggaagcc agcatcacgg ttagccatag cttccagtgc cacccccgcg acgcgctccg
    18001 ggcgctctgc gcggcgctgc tcacctcggc ggctacctcc cgcaactctt tggccagctc
    18061 cacccatgcc gcccctgtct ggcgctgggc tttcagccac tccgccgcct gcgcctcgct
    18121 ggcctgctgg gtctggctca tgacctgccg ggcttcgtcg gccagtgtcg ccatgctctg
    18181 ggccagcggt tcgatctgct ccgctaactc gttgatgcct ctggatttct tcactctgtc
    18241 gattgcgttc atggtctatt gcctcccggt attcctgtaa gtcgatgatc tgggcgttgg
    18301 cggtgtcgat gttcagggcc acgtctgccc ggtcggtgcg gatgccccgg ccttccatct
    18361 ccaccacgtt cggccccagg tgaacaccgg gcaggcgctc gatgccctgc gcctcaagtg
    18421 ttctgtggtc aatgcgggcg tcgtggcctg cccgctctaa tgcccggttg gcatggtcgg
    18481 cccatgcctc gcgggtctgc tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg
    18541 ccccgccctt ctccggggtc ttgccgttgt accgcttgaa ccactgagcg gcgggccgct
    18601 cgatgccgtc attgatccgc tcggagatca tcaggtggca gtgcgggttc tcgccgccac
    18661 cggcatggat ggccagcgta tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact
    18721 cggacgccag cgccttctgc tggtcgaggg tcagctcgac cggcagggca aattcgacct
    18781 ccttgaacag ccgcccattg gcgcgttcat acaggtcggc agcatcccag tagtcggcgg
    18841 gccgctcgac gaactccggc atgtgcccgg attcggcgtg caagacttca tccatgtcgc
    18901 gggcatactt gccttcgcgc tggatgaagt cggccttggc cctggccgat tggccgcccg
    18961 acctgctgcc ggttttcgcc gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt
    19021 gcttttcggc tccatgcaat ggccctcgga gagcgcaccg cccgaagggt ggccgttagg
    19081 ccagtttctc gaagagaaac cggtaagtgc gccctcccct acaaagtagg gtcgggattg
    19141 ccgccgctgt gcctccatga tagcctacga gacagcacat taacaatggg gtgtcaagat
    19201 ggttaagggg agcaacaagg cggcggatcg gctggccaag ctcgaagaac aacgagcgcg
    19261 aatcaatgcc gaaattcagc gggtgcgggc aagggaacag cagcaagagc gcaagaacga
    19321 aacaaggcgc aaggtgctgg tgggggccat gattttggcc aaggtgaaca gcagcgagtg
    19381 gccggaggat cggctcatgg cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc
    19441 cttgttcggt ctgccgccac gccagaagga tgagccgggc tgaatgatcg accgagacag
    19501 gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg
    19561 gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc
    19621 gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca
    19681 gctatccggc ctctggccgg gcatattggg caagggcagc agcgccccac aagggcgctg
    19741 ataaccgcgc ctagtggatt attcttagat aatcatggat ggatttttcc aacaccccgc
    19801 cagcccccgc ccctgctggg tttgcaggtt tgggggcgtg acagttattg caggggttcg
    19861 tgacagttat tgcagggggg cgtgacagtt attgcagggg ttcgtgacag ttagtacggg
    19921 agtgacgggc actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag
    19981 aactttccgc taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc
    20041 ctcatgtggc ggccctcgcg agaattaatt cagataaaaa aaatccttag ctttcgctaa
    20101 ggatgatttc tagcgatgac cctgctgatt ggttcgctga ccgtagactg gttggcttgg
    20161 tttcatcagc catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca
    20221 gagcaggatt cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc
    20281 cgctcgcggg tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag
    20341 gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg tgcgacatcg cgcgcgcgcg
    20401 cgcggatgct agcggcgcgc caaaaaaaag cccgctcatt a
//
