LOCUS       sequence-464764-       20110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0482) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20110)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.10-Dadh-Delta-Elen0482(ElA2)
FEATURES             Location/Qualifiers
     source          1..20110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      13734..13761
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      13893..13920
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14352..15203)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15190..16029)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16540..17511)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            18750..18837
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19179..19573
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(19736..19779)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            19875..19984
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatatgagtg cttggactgc tgcgtccgtc gaagacgcgg cgaacaatct tcttgaagaa
     8701 ctgaatagcg gcgcgatgcg cttgtgcgtg gagcatgacg cgtccgacga cgttgcgccg
     8761 gcgttgctgc gcgcggctcg cgctcgcgtc gaggcggggt cgaacgtgcg cgtcgtgtgc
     8821 gccgacgccg ttgcagctgc gcgctttcgg gcgcttgcgg ccgacgatcc gctgctgggc
     8881 gcgctcgacg tggtatcggt gcgcgagctg gccctctcga ttctaggcga cgcgcgggtc
     8941 ggcgatgccg tgggacgcga cgcgcgcgtg ctggacgaga acgagctggc cgtgctcatg
     9001 gaggacgtga aggtgagcgg cctcaagccg ggccggctgc gcgagatgct caagttcttc
     9061 tacaagagca tcagcgactg cgccgacgat gacgagcgct ggctgatcac cgccgaagag
     9121 cagaccgtgc acgccatcct caccgagaac ctggaggcgc gccgcgcgtt gctgccgtgc
     9181 gaggtttcgt cgctggcgta tcgcggcctt gtgaaagccg gggtcgagcg cgagccgctg
     9241 acgctggtgg cagacgactt cgggtcgctc agcaaggcgt cgcagcgcct cgtgcgccat
     9301 ctggcgacgg acggcctcgt ggcggcgggc cgcacgctgg cctcgtccag ctccgaggag
     9361 ccgtatccgt gcttcgacgg gttccgcgcg cttgccgacg aggccgactg cctggtgacg
     9421 ctggcgtgcg agcggccggc cgcggcgcgc gagtcccgcg cgctggacga cccggcggcc
     9481 gagttcgcgt tcgcggcggc gtccgtggag gagtgcctgg ccgacggctt ccggccaggc
     9541 gacgtggctg tggccgtgcc gaacgccgct tggggcgagc gcatcgccgc cgagctggaa
     9601 gggcgcggca tcgccgtgga gcgcgggttc gacagcggga agatcaaggg cgacccgcgc
     9661 accgagggcc gttacgccga cctcaagctg gccgcgttcc tgcggttgta cctcgatccg
     9721 cacgatttca cgtcgttgcg ctcgtggctg gggttcggcg attggctgct gcgctccgat
     9781 gcgtttttgg agctgatggc gtacgcgcgc gaccatgaga cgaccgtgcc cgaagccgtg
     9841 gcgcagctgc gcacgcagcg cgacgccgat cgctcctcga cgatattcgg gaagttcgac
     9901 ggcccgctcg acgagctgga cgagctgctg cgcgcctgcg aggaagggct gacgcgcgag
     9961 gccgccgtag cgctgttcga agcgcacgga atgccgttgt ccccgcgcca cgtggagctg
    10021 ctgggcgacg accctgcgca tgcggacgtc gagcgcttgg cgcgctgcgg gttcgcgaag
    10081 ccggtggagg atgtcgcgcg cgatgccgtg catgtggtct cctacgcgcg ctgccacggg
    10141 caccatgtgc gcacggtgtt cgtcaccggc ctggtgaacg gcttcttgcc ggcgagcgac
    10201 gccgtggacg acaagttcac catcgaccat cggcgcgtgg cgctcgaacg cgagcgcgcg
    10261 ctgttcctgg acgtgctgtc ctgtgcgagc gaacgagcgg tatgcacgcg gttcgtgcgc
    10321 gaccttttag agaacaccgc gttcacgaag gtgcagacct cgcgcgtgtt cgtgcgcgac
    10381 ggcgagcgct tcgcgcgcgt gacgccgtcg gagttcgcgt cgctgaccga cgaggtgctc
    10441 tctcccgcgc ccgacgcgcc tcgcgagctg ccgacgaagg tgctgtacgc gtcttcgact
    10501 ttgtagctcc aacggtctga cgaccgcgcg aaccttcgct gacttttcct gaccggtgat
    10561 tttgaaatga tgaaaggact gatagatatg agcgatgttg atgtattcgt gatcgatgac
    10621 gatttcgatc ccctggccga cctgagcctg gacgacggcg agcgggacga tgccgaggcg
    10681 gattacctgc cgcccatccc cgatgccgac aagagcgtgg tgcctcccgt ggtgccgttg
    10741 tctgcgaccg agcgcatcga gaagctgctg gcgggcattc ccggccagca gttccgcctg
    10801 ctgcacgccg tggaattctg caccgagccg aagacgatgg acgaggccgt ggccaatttg
    10861 gacgcggcgt atccgaacac gacgagcgtg tacggctcgg cgcaggtggt gcagctgctc
    10921 gagcgcgacg gcgcgctgga gcgcatcgtg gacgaggacg ccgcccatgc ggccgccggc
    10981 gacgctgccg atacgcccgc cgaggacgag ggcttcatct cggtgacgcc cgctccgccg
    11041 tgtcactacc gtgccacgca agcgggcctc gatgccgtcg cggcgcacgt caacgagggc
    11101 ctcgtggcgg agcggatcag cgaagacgag cgctatctgc ccatcttcca gcgcgtgctt
    11161 gagatgtgcg cgcgcgaagg aggctgcccc accaaggagc tggaccaggc ggtggacggc
    11221 gacccgctgt gccaagagcc gcgccgcttc tgcgggttct tccgcggcaa gctggaagaa
    11281 acaggcgcca tcgaatggcg cgacgcctgg accattaccg atctggggcg cagcgtgctc
    11341 gcgtccggct tgttcgccgc cgcttccccg tccgaacgct agagaaggag ccacccatgt
    11401 cccacgatca ggaaaccacc atcgccgacg tgctgcgcgg ccgcatggca acctaccagt
    11461 tcctctcgcg cctgttccgc gtggaggtgg accaggagct gtacgacacg ctgatgtcca
    11521 tgcgcttccc ggcgaacacc ggcaacgcgc tggtggacga aggctaccgg atgatctgcg
    11581 gttatctgag ccaagccgac ggcacggtgc tcaccgagct ggcggtggac tacgtgcgcg
    11641 cgttcatcgg gtcgggcaac gacggcttct cggccgcgta cccgtacgag tcggtgtaca
    11701 cgagcccgaa gcgcctcatg atgcaggacg cccgcgacga ggtgctggtg ctgtaccgcg
    11761 ccttcggtct ggacaagcag gaaagctgga aggagggcga ggaccatatc gcgctggagc
    11821 tggagttcga gcagatccta tgcgagcgcg ctatcagggc gtacgaggcc ggcgacgagg
    11881 acgagtgcct gaagctgctg ctgtcgcagc gcaacttcct ggaggaccac ctgctggcgt
    11941 ggtaccccat gatggcggcc gacttgcaga agttcccgca gaccgacttc tacaagggcc
    12001 tgggcaagct gaccgacggc ttcctgcgca acgaccgcga atttttggac gccgtgctgt
    12061 ctgagaacga agcggattgc catccagagc ggaggccgca ggccgaagcc gaaggatccc
    12121 gtgcggcgtc ggccgaaacc gaagtcgagg ttgcatgatg gacgagcacg gcatcgagcc
    12181 caagcgcgat acgggcccct gcgtgtcgcg ccgcacgttc gccctgggag ccgtgggagc
    12241 gtgcgccgtc atcgggctgg gcggcgtgaa gtacctgccc agtgccacgt tgctgcgccc
    12301 gccgggcggg caggacgagg agcagctggc gcgcggctgc ctgcattgcg agaagtgccg
    12361 cgaggtgtgc ccgaagcatg ccatcgctcc tgctcatctt gaggacggca tcttgaacgc
    12421 gcgcacaccg cgcatggact tcaaaagcgg ctggtgcgat ttctgcgaga acgagccggg
    12481 cgggccgaag tgcgtcgcgg cgtgcccgac gcatgcgctt tcgtgccccg acccgtcgca
    12541 ggcgatcatc ggcaaggcgg agatcaaccg cgactggtgc cttgccgcga agggcatggg
    12601 ctgccacgag tgcgtcgacg tgtgcaacta cgaggcgctc gagctgggcg ccgacaacgt
    12661 gccggtggtg gacgtggatg cgtgcaacgg ctgcggcgcc tgcgagctgg cgtgcatctc
    12721 gctgtcggca ggttccatca cggccggcgc caccgatcgc gccatcgtcg tccgtccgac
    12781 cgaagaagtg gaggcgtgat caggatgaag aagctcaaaa cctatcggct gcggttcctg
    12841 atcatgctgg cgctgctggc cgtggtggcc gtggggtact tcacggccgg cggcatcggc
    12901 aacttctgcg gcatcggctt cgagtccatc acgctgctgt gccccctagg cgcgctgctg
    12961 gccatgatcg ccgagcgcac agccatcccg atggccgtga tcagcgttgc ggtcgtgctg
    13021 ctggtgtgta tcgtgctagg caaggtgttc tgcgcctggg cgtgccccgt gcacttcctg
    13081 tcgcgaggaa ggaagaaggg ggataagggg gacaaggagg gtaaaggggg caaggttgct
    13141 tccccttgct ccgcgtgcgc ttccccttgc ggcaaagcca agggcatcaa gatcgacagc
    13201 cgtcacggca tcctcgctgc ggcgttgggc tccacgctcg tcttcggctt cccggtgttc
    13261 tgcctcgtct gcccgatcgg cctcacgttc gccacggtgc tgctggtcat gcgcctgttc
    13321 gcgttcggcg acgccacgtg gacggtcgtc gccttcccgg ccatcatcgc gctggagatc
    13381 ctgctgctgc cgaagtggtg ccagcgcttc tgcccgttgg gcgcgttgct ttcgctgttc
    13441 agcgggctga accggacgtt ccgcccccag gtggacgccg agaagtgcct gcgcgagggt
    13501 cgcggcaagg cctgcaacct gtgcgagcag gcatgccccg agggcatcaa cctgcacgat
    13561 gtcgcggcgg gcgagaccac gctcaacgac tgcagcaagt gccgcgcgtg cgccgacgtg
    13621 tgccccgagc gcgccatcac gttcccgttg ctgccgaaga agcaggcgca ggcgacgccg
    13681 ctgcccgtcg acgacgagat cccggaaagg ggatagacta gtcgactcac actggctcac
    13741 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
    13801 aatccggctt ttttattatt taattcctgt tataaaaaaa ggatcaattt tgaactctct
    13861 cccaaagttg atcccttaac gatccctgca ggagaaggcc atcctgacgg atggcctttt
    13921 tgaagctttc tagaatcggg ggcccccggg gggattctag aacaatggaa gtcctcgtct
    13981 cgccctcgaa ttagcccgct taatgagcgg gctttttttg aattaattct cgcgagctgg
    14041 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
    14101 cgcgaattgc aagctggccg acgcgctggg ctacgtcttg ctggcgttcg ggagcagaag
    14161 agcatacatc tggaagcaaa gccaggaaag cggcctatgg agctgtgcgg cagcgctcag
    14221 taggcaattt ttcaaaatat tgttaagcct tttctgagca tggtattttt catggtatta
    14281 ccaattagca ggaaaataag ccattgaata taaaagataa aaatgtcttg tttacaatag
    14341 agtggggggg gtcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac
    14401 tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg
    14461 cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct
    14521 atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg
    14581 cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg
    14641 ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc
    14701 tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg
    14761 atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca
    14821 ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg
    14881 gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct
    14941 tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct
    15001 tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg
    15061 ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttgagg
    15121 gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gtcggacgtg gctgaggctg
    15181 tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc tctccagcac
    15241 ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga acggtgcgcc
    15301 atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt cgtccacacc
    15361 ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc ggatgttatc
    15421 gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca tcatggccgc
    15481 gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg cgatggcctc
    15541 catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga tgtggaaccg
    15601 gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga ggccgtcgaa
    15661 ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca gcaggccgtc
    15721 agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca ggcggtgatg
    15781 aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg gcagttcgcc
    15841 cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg ccagcatgga
    15901 tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca tgttgggcaa
    15961 aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga taggcttatg
    16021 ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc ggggtcacta
    16081 cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc gtattcgtcg
    16141 tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc ggcatatcgc
    16201 gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc cttttggtct
    16261 ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc ggaacaagga
    16321 caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc ggccagcctc
    16381 ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt cacttttgat
    16441 caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt ctgtgcgtga
    16501 gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga aatctggccc
    16561 gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg cccgcgcaag
    16621 ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat ccgcttcgtg
    16681 gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc cgatttcctc
    16741 ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact tgctgaggtc
    16801 atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca gcgccgtgcg
    16861 ccggtggcgg ctaagctgcc gctcggacag ttcgaggctg gccagcctgc gggccttctc
    16921 ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg ccgggccagc
    16981 ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac cggcgcgggt
    17041 ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc ggctgtcggc
    17101 gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc cccgaagttc
    17161 accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc tggtttccac
    17221 taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact cgctgaggtc
    17281 gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca cgtcattgcc
    17341 ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg ctgaccattc
    17401 ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc gctcacagcc
    17461 catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca tcgggccacc
    17521 aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg tcaggtcgag caagagcaac
    17581 gatgcgatca gcagcaccac cgtaggcatc atggaagcca gcatcacggt tagccatagc
    17641 ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg cggcgctgct cacctcggcg
    17701 gctacctccc gcaactcttt ggccagctcc acccatgccg cccctgtctg gcgctgggct
    17761 ttcagccact ccgccgcctg cgcctcgctg gcctgctggg tctggctcat gacctgccgg
    17821 gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt cgatctgctc cgctaactcg
    17881 ttgatgcctc tggatttctt cactctgtcg attgcgttca tggtctattg cctcccggta
    17941 ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg ttcagggcca cgtctgcccg
    18001 gtcggtgcgg atgccccggc cttccatctc caccacgttc ggccccaggt gaacaccggg
    18061 caggcgctcg atgccctgcg cctcaagtgt tctgtggtca atgcgggcgt cgtggcctgc
    18121 ccgctctaat gcccggttgg catggtcggc ccatgcctcg cgggtctgct caagccatgc
    18181 cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc tccggggtct tgccgttgta
    18241 ccgcttgaac cactgagcgg cgggccgctc gatgccgtca ttgatccgct cggagatcat
    18301 caggtggcag tgcgggttct cgccgccacc ggcatggatg gccagcgtat acggcaggcg
    18361 ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc gccttctgct ggtcgagggt
    18421 cagctcgacc ggcagggcaa attcgacctc cttgaacagc cgcccattgg cgcgttcata
    18481 caggtcggca gcatcccagt agtcggcggg ccgctcgacg aactccggca tgtgcccgga
    18541 ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg ccttcgcgct ggatgaagtc
    18601 ggccttggcc ctggccgatt ggccgcccga cctgctgccg gttttcgccg taaggtgata
    18661 aatcgccatg ctgcctcgct gttgcttttg cttttcggct ccatgcaatg gccctcggag
    18721 agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg aagagaaacc ggtaagtgcg
    18781 ccctccccta caaagtaggg tcgggattgc cgccgctgtg cctccatgat agcctacgag
    18841 acagcacatt aacaatgggg tgtcaagatg gttaagggga gcaacaaggc ggcggatcgg
    18901 ctggccaagc tcgaagaaca acgagcgcga atcaatgccg aaattcagcg ggtgcgggca
    18961 agggaacagc agcaagagcg caagaacgaa acaaggcgca aggtgctggt gggggccatg
    19021 attttggcca aggtgaacag cagcgagtgg ccggaggatc ggctcatggc ggcaatggat
    19081 gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc tgccgccacg ccagaaggat
    19141 gagccgggct gaatgatcga ccgagacagg ccctgcgggg ctgcacacgc gcccccaccc
    19201 ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc tccagcgtat ttctgcgggg
    19261 tttggtgtgg ggtttagcgg gctttgcccg cctttccccc tgccgcgcag cggtggggcg
    19321 gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc tctggccggg catattgggc
    19381 aagggcagca gcgccccaca agggcgctga taaccgcgcc tagtggatta ttcttagata
    19441 atcatggatg gatttttcca acaccccgcc agcccccgcc cctgctgggt ttgcaggttt
    19501 gggggcgtga cagttattgc aggggttcgt gacagttatt gcaggggggc gtgacagtta
    19561 ttgcaggggt tcgtgacagt tagtacggga gtgacgggca ctggctggca atgtctagca
    19621 acggcaggca tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa
    19681 acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg gccctcgcga gaattaattc
    19741 agataaaaaa aatccttagc tttcgctaag gatgatttct agcgatgacc ctgctgattg
    19801 gttcgctgac cgtagactgg ttggcttggt ttcatcagcc atccgcttgc cctcatctgt
    19861 tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg
    19921 cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct
    19981 gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc
    20041 tgtatatcgt gcgacatcgc gcgcgcgcgc gcggatgcta gcggcgcgcc aaaaaaaagc
    20101 ccgctcatta
//
