LOCUS       sequence-464765-       13578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13578)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..13578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     regulatory      4350..4359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      7202..7229
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7361..7388
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7820..8671)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8658..9497)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10008..10979)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12218..12305
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      12647..13041
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13204..13247)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13343..13452
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcag
     1501 gctttttcgt ctcggcggtg gcgcatgatg atttcgcgtg attcatcaag tgcttcaatg
     1561 agcggcttga ggcgttcttc gttttcctgc ttgtagatgc agtagatgat gaaatgcgca
     1621 tcctcgtccg aaatggggat gcttacggtt tggtttacat cttcgagaaa tttgagttgc
     1681 cgaagattct tctgcaagat gagcacgccg ccgtcaggag aggtagtcgc atagcttgtc
     1741 acggcacgcc ctagtttggg acgttttttt ggctcgaatc cgtgttcgtg gcatatctgc
     1801 tcgatccgtc tccatcccga aacggagtat tcatcgatga actggataaa cgtctcgtca
     1861 gctagttcgt ctatgctgat ttccgagcga tcagcaagtg gatggtcgcg atcaacgacg
     1921 gcaacaaatg gcgtttgaac aagcgggcgg aaaaccagcc ctcgctcttc gagcgcatga
     1981 gcctcgtcgt acgcgaacac catatccacc tcgtctcgat ccaactgatc gaacagaggt
     2041 cgatcctgat ggtgctcaaa cagaatgggt acatggtgct ggtcattgag cagcacggtg
     2101 gacagcgaga tgatgctcga caccgtgttg tcgtagagta tcccgtctac ccggatgggc
     2161 cgttctttca cgaccgtggc tatggctgct tgggccctgt cgtatgcatc gaccatctcc
     2221 atcgcgcatc cgaacagaat acgtcctgct tcgctcagtt gcacggttct tcggtcgcgg
     2281 atgaataact cggcgtcgaa ctcgcgttcc aacgcaacga tatgcttgct cagcgcaggc
     2341 tgggtcatat gcagcttctc agctgcttcg gtgtagttga ggcagcgcgc caaaacgatg
     2401 aactcgcgat agaagtcgat gttcatagga tccccttccg cccgttagag cgttccccgc
     2461 ttcatggtat cacgtcgacg gcgtccgcgc gtttccggat tgtctgtggt agaaaacgga
     2521 ataacgatat gcaccttcat ctcggttggc ctgcgacgtt cgttttcatt atttccatat
     2581 gctttggctg ctcgccatac tcaggtttaa ggaaagcctt gagagaagga gggtgtatgg
     2641 ggaagacgac cgtgacaagg cgcgcgttcg ctcagcttgc cgccgcgaca ggcgcaatag
     2701 ccgctatggg tgttggaact cgtcctgcgg tggcgcttac cgatggcaat tcggggacgg
     2761 cgggcgagcg ggggatcaag aagatacgct cgtgctgccg cggctgcgga aaggtcgaat
     2821 gcggtgtgtg ggtgtacatc caagatggca aagtggttcg aaccgaaggt gacgaaacct
     2881 gcttcaacac tatgggcaat cattgcagca aggggcaagc gtccattcag gctgcatatc
     2941 atcctgatcg tatcaagttc ccaatgaagc gcacgaaccc gaagggcagc gaagatccag
     3001 gttgggtgag gatcagctgg gacgaggcct accggaccat tgcggacaat attatgcagt
     3061 tgcgtgagaa gtacggtcct gaaagcttgt tcacgtggtg cggtacgggc cgacagtggt
     3121 gcatgcagtc cgatgctggc atggcgcttg agctgttcgg tacgccgaac atcatcgcgg
     3181 cgtaccaagt gtgcaagggt ccgcgccatt tttcgtcgcg tctcgacaac gttcaggcgt
     3241 ggtcttggag tgaggtgata aaccattcga cgaaatacgt ccaatgggct accgatccct
     3301 ccgtttcgaa ttacgacgat tcgtctcgtt tggtaattga tgtggcgcgc gaagctgagg
     3361 cttttatcgt ggttgaccct cgcctgtcga atcttggccg cactgcgaag tattggttaa
     3421 acttgcgacc tggcaccgat aatgccatgg cgctaggatg gtgccatatc atattgaagc
     3481 acgatctggt ggattggcag ttcgtgaaac gctggtccaa tgcctcgttc atcgtcgtgc
     3541 ccgatatgga cccttcgggc tacaccgagg cggttcaaaa cacgaaaagc ccctacgaat
     3601 atcgtacgcg tttgctgacc gaagccgaca tagatccttc tatggttgac tgggagattg
     3661 aaggcgaggg gaatccaaaa cgttatcttg tgtacgatca gatcaatcgg cgctggacat
     3721 attggcaagc cgatcccgaa gacgcgcatt gggaaggcga gacctggaca aaacaaacct
     3781 ctggattcac gcaagacgtt tcgcgtcttc gcgacgacga atcgaaggtg gctggctgga
     3841 tcgcggatct gtcggaattc gatccgcgta tcgatccggc gctcaccggg gaattcgaag
     3901 taaggcttaa ggacggaagt acgcatatcg gccgtcctgc atgggatttg tgggctgagt
     3961 acctgcaaca attcactccc gaacgagtgt cggagatcac gggcgtggat gcccagctta
     4021 tcgaagacgc cgtggttgaa tgggcaacgc gcgatgaccc gcgcataccc aacggcggta
     4081 taaattacgg tctgggcgtg gagcatgcgg gcaactcaac gcagaattgc agggccatta
     4141 tggcggcttg cgcaatggta ggcgccatcg acacgccggg cggccagcgc ggtgcgacga
     4201 acggatggac cgaacagtcg ggtccgtgcg ccatgcttcc gtcgatggcg gcattcgcgt
     4261 tcatgcccac gcccgacttg tcgctcaaga tggccggaaa tgagaagatg ccgcttctat
     4321 actggtacgg tgtgtggtgc gatgccaatg ccgccatgga atgcgcgcac aaggagccgg
     4381 atgcgcccta tgagattcat ggtggcatga tcggctcggg cgaccatatg aacatgggca
     4441 acgcaacgta caattgggaa gcgctcaaca tgctcgactt tctgttcgag gcgaatctgt
     4501 ggcattcccc tacttcaggt gcggccgaca tcctccttcc ggtctgccat tggaccgaga
     4561 tcaacgcgta tcgcattgcc caaggggcat ccggtgggtt tggcctatgc gtgaaagctg
     4621 ttgatcctcc tggcgaatgc aaaagcgatc cgttgtactt catggagctg tcgaagtatt
     4681 tcggcgtccc agcgtttgac ggcgacgatc cgtggctcga gaacaaacct gatgccgatc
     4741 tcgaaatcga aaacctcacg attcagtgct gtgtccaagg atgcgcgcca tacaacaatt
     4801 ggaacgagtt ggaggctgca ttccaagagc acggttggtg gaacatgaaa cgcgagatcc
     4861 cggaagactg gggcacgtac cgtcgatacg aggtggggca ggcgtatcgg ttggctcccc
     4921 atcagcaacc ggctcagctg aacatcaata aaccggggtt ccccacacct acgatgaaac
     4981 atgagttttg gtgcacttcc atcgaatcgt tcttcccgga gggggcggat ggccctgagc
     5041 ttgcacccgg gttcacttcc gaagcgctgc cttattatgc agagcccgca cacggccctg
     5101 tggtcgatgc ggaaacgtac aaggagtatc ccattacctg catcactgga cgacgcatac
     5161 cggtgtactt ccactccgag caccgacagc tgccctggtg tcgcgagctt tggccggtgc
     5221 ctcgcatgga gatcaatccc gatacggctg ctgaacttgg actcgaacaa ggagattggg
     5281 cctggatcga gagcccttgg ggcaaggtgc gacagacggt cgatctctat tacggcatta
     5341 agccgaacat gatcaacgcc gagcatcaat ggtggtatcc ggagttggct caagcggaca
     5401 aaggatatga gttgtcatgc atcaattgca ttaccgatcg gaagactcag gataaataca
     5461 atggatcgtc gaatgtgcgc acctatccag tgaaggtgta caaagccacg cctgagaatt
     5521 ccccctttgg gaatcctatc ccttgcggaa acgatgggac tgagatcatt catgactctt
     5581 ctgatcctcg cctcaagcta tgggaaatcg gcgctgctgg catccatccg gatcacttcg
     5641 agtaggagag ggggaattat gacgcagtac gctatcgtca ccgatctcga tcgttgcgta
     5701 ggttgtcttg cctgttcggt tgcctgcaag gtagtcaacg gcgttccgac gggctcgttt
     5761 tggaacaaga cattgcgcat tggcccgaat cctcgagaag gcggcagtgg gcaatggccc
     5821 gatgtggagc tgtattttct caatgtgcag tgccagcact gcgagaaccc cgaatgcgtg
     5881 aaggtgtgcc cgacgggcgc ctcgcacaag accgaggacg gcaccgtgca gatcgacaag
     5941 tcgaagtgca tcggctgcca gttctgcgcg atgtcgtgcc cgtacggggt gcgctacctc
     6001 aacgaggagg agcgcgtcgt ggagaagtgc accttgtgcg agcagaagat cgctcagggc
     6061 gaacttcctc agtgcgtgag tcaatgctgc ggtatggcac gctggttcgg cgatgcggac
     6121 gaaggtatcg aaggtttcgt cgggcctcgc ggcgagatac tgggcgagta tatatcgcca
     6181 ttctcggatg atcaggtgta tagcctgccg gatgtgggca ataagccgtc gctcaggtac
     6241 atcttgcgcg atatgaaatg gcaaggaggc ggtgaatgat ggaactgcaa tggcctttga
     6301 ttttgttcac cacgctggtt gcgtggagcg cggggctgtt cgggacgcag gcgctgatgg
     6361 cggtgttcgg cgtcgggaag aaggcgcagg tgccggcttg ggtttgctcg gccgtcttgc
     6421 tggcggccgg cgggatcgcg gtgttcttcc atctcgagca ctgggagcgg atcttcaacg
     6481 ggttcgggca tttgacgagc gggatcacgc aggaattgat cgcgatcgtg gtcttggccg
     6541 tggtggctat cgtctacttg gtgctcatgc gcaagtccga cgacggcgcc agcgtgccga
     6601 agtggctggc atgggtctcc gtcgccttgt cggtcgtgct cgtggccgtc atggcgcaca
     6661 gctacacgat ggccgcccgc cccgcctggg actcggccct ctggatcctc tacgtcctcg
     6721 gcaacgcctg cgtgttgggc cccgcgacgt tcgccctcct ctccgccttg gctgcagggg
     6781 gaccccggga ccagcccgcc gagcgcgcag ccgacgccgg ggcccccgcg ggtctcgcgg
     6841 ccctcgccgg cgccgccctc aacgccctcg cggccctcgc cttcgccgcc ttcctccagc
     6901 tctccgccgg ctccttcgcc gacgtcggcc tctacttcga cccgacgcac cccacgaagg
     6961 ccatggccga cgccgccgcg acggtcgcct cccaggcccc gctgctgtgg ctgggcgccg
     7021 tggccgtggg cgcgatcgtg cccctggccg ccgccttcct aggcaggcgc acggggaact
     7081 ggaagctctg ggtcccggtc gcgatagccg ccgccctggt cggcgccgtc tgcatgcgcg
     7141 tcgtcttcta caacctgggg ctcagcgtgt tcatgttcta ctagactagt cgactcacac
     7201 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
     7261 agattattaa tccggctttt ttattattta attcctgtta taaaaaaagg atcaattttg
     7321 aactctctcc caaagttgat cccttaacga tccctgcagg agaaggccat cctgacggat
     7381 ggcctttttg aagctttcta gaatcggggg cccccggggg gattctagaa caatggaagt
     7441 cctcgtctcg ccctcgaatt agcccgctta atgagcgggc tttttttgaa ttaattctcg
     7501 cgagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7561 taagttagcg cgaattgcaa gctggccgac gcgctgggct acgtcttgct ggcgttcggg
     7621 agcagaagag catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca
     7681 gcgctcagta ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca
     7741 tggtattacc aattagcagg aaaataagcc attgaatata aaagataaaa atgtcttgtt
     7801 tacaatagag tggggggggt cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg
     7861 ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc
     7921 gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca tagccgcaca
     7981 aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac agccgctggt
     8041 gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg atgcgcacat
     8101 gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc
     8161 cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc ttgcggccat
     8221 tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg
     8281 ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag ctacctttga
     8341 ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc cggagctgcc
     8401 gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca gccatggcca
     8461 ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg ctgaactcga
     8521 tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc
     8581 gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgt cggacgtggc
     8641 tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt gcgctgcctc
     8701 tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg atcagcgaac
     8761 ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg ctggtcgtcg
     8821 tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg
     8881 atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc
     8941 atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt atcggcggcg
     9001 atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc ttcctcgatg
     9061 tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc gcgcttgagg
     9121 ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg ctggatcagc
     9181 aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg
     9241 cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc
     9301 agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag ttgcagggcc
     9361 agcatggatt taccggcacc accgggcgac accagcgccc cgaccgtacc ggccaccatg
     9421 ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga acgcctccag aatattgata
     9481 ggcttatggg tagccattga ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg
     9541 ggtcactacc cccgccctgc gccgctctga gttcttccag gcactcgcgc agcgcctcgt
     9601 attcgtcgtc ggtcagccag aacttgcgct gacgcatccc tttggccttc atgcgctcgg
     9661 catatcgcgc ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct
     9721 tttggtcttt catatcagtc accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg
     9781 aacaaggaca aggtgcagcc gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg
     9841 ccagcctcgg ccttgtttga cgtataacca aagccaccgg gcaaccaata gcccttgtca
     9901 cttttgatca ggtagaccga ccctgaagcg cttttttcgt attccataaa acccccttct
     9961 gtgcgtgagt actcatagta taacaggcgt gagtaccaac gcaagcacta catgctgaaa
    10021 tctggcccgc ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc cagctcggcc
    10081 cgcgcaagct ggacgctggg cagacccatg accttgctga cggtgcgctc gatgtaatcc
    10141 gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg cctcggccat ggccttgccg
    10201 atttcctcgg cactgcggcc ccggctggcc agcttctgcg cggcgataaa gtcgcacttg
    10261 ctgaggtcat caccgaagcg cttgaccagc ccggccatct cgctgcggta ctcgtccagc
    10321 gccgtgcgcc ggtggcggct aagctgccgc tcggacagtt cgaggctggc cagcctgcgg
    10381 gccttctcct gctgccgctg ggcctgctcg atctgctggc cagcctgctg caccagcgcc
    10441 gggccagcgg tggcggtctt gcccttggat tcacgcagca gcacccacgg ctgataaccg
    10501 gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg
    10561 ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc
    10621 cgaagttcac cgcctgcggc gtcggccacc ttgacccatg cctgatagtt cttcgggctg
    10681 gtttccacta ccagggcagg ctcccggccc tcggctttca tgtcatccag gtcaaactcg
    10741 ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct cggcgggcct gatatacacg
    10801 tcattgccct gggcattcat ccgcttgagc catggcgtgt tctggagcac ttcggcggct
    10861 gaccattccc ggttcatcat ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc
    10921 tcacagccca tggccttgag ctgtcggcct atggcctgca aagtcctgtc gttcttcatc
    10981 gggccaccaa gcgcagccag atcgagccgt cctcggttgt cagtggcgtc aggtcgagca
    11041 agagcaacga tgcgatcagc agcaccaccg taggcatcat ggaagccagc atcacggtta
    11101 gccatagctt ccagtgccac ccccgcgacg cgctccgggc gctctgcgcg gcgctgctca
    11161 cctcggcggc tacctcccgc aactctttgg ccagctccac ccatgccgcc cctgtctggc
    11221 gctgggcttt cagccactcc gccgcctgcg cctcgctggc ctgctgggtc tggctcatga
    11281 cctgccgggc ttcgtcggcc agtgtcgcca tgctctgggc cagcggttcg atctgctccg
    11341 ctaactcgtt gatgcctctg gatttcttca ctctgtcgat tgcgttcatg gtctattgcc
    11401 tcccggtatt cctgtaagtc gatgatctgg gcgttggcgg tgtcgatgtt cagggccacg
    11461 tctgcccggt cggtgcggat gccccggcct tccatctcca ccacgttcgg ccccaggtga
    11521 acaccgggca ggcgctcgat gccctgcgcc tcaagtgttc tgtggtcaat gcgggcgtcg
    11581 tggcctgccc gctctaatgc ccggttggca tggtcggccc atgcctcgcg ggtctgctca
    11641 agccatgcct tgggcttgag cgcttcggtc ttctgtgccc cgcccttctc cggggtcttg
    11701 ccgttgtacc gcttgaacca ctgagcggcg ggccgctcga tgccgtcatt gatccgctcg
    11761 gagatcatca ggtggcagtg cgggttctcg ccgccaccgg catggatggc cagcgtatac
    11821 ggcaggcgct cggcaccggt caggtgctgg gcgaactcgg acgccagcgc cttctgctgg
    11881 tcgagggtca gctcgaccgg cagggcaaat tcgacctcct tgaacagccg cccattggcg
    11941 cgttcataca ggtcggcagc atcccagtag tcggcgggcc gctcgacgaa ctccggcatg
    12001 tgcccggatt cggcgtgcaa gacttcatcc atgtcgcggg catacttgcc ttcgcgctgg
    12061 atgaagtcgg ccttggccct ggccgattgg ccgcccgacc tgctgccggt tttcgccgta
    12121 aggtgataaa tcgccatgct gcctcgctgt tgcttttgct tttcggctcc atgcaatggc
    12181 cctcggagag cgcaccgccc gaagggtggc cgttaggcca gtttctcgaa gagaaaccgg
    12241 taagtgcgcc ctcccctaca aagtagggtc gggattgccg ccgctgtgcc tccatgatag
    12301 cctacgagac agcacattaa caatggggtg tcaagatggt taaggggagc aacaaggcgg
    12361 cggatcggct ggccaagctc gaagaacaac gagcgcgaat caatgccgaa attcagcggg
    12421 tgcgggcaag ggaacagcag caagagcgca agaacgaaac aaggcgcaag gtgctggtgg
    12481 gggccatgat tttggccaag gtgaacagca gcgagtggcc ggaggatcgg ctcatggcgg
    12541 caatggatgc gtaccttgaa cgcgaccacg accgcgcctt gttcggtctg ccgccacgcc
    12601 agaaggatga gccgggctga atgatcgacc gagacaggcc ctgcggggct gcacacgcgc
    12661 ccccaccctt cgggtagggg gaaaggccgc taaagcggct aaaagcgctc cagcgtattt
    12721 ctgcggggtt tggtgtgggg tttagcgggc tttgcccgcc tttccccctg ccgcgcagcg
    12781 gtggggcggt gtgtagccta gcgcagcgaa tagaccagct atccggcctc tggccgggca
    12841 tattgggcaa gggcagcagc gccccacaag ggcgctgata accgcgccta gtggattatt
    12901 cttagataat catggatgga tttttccaac accccgccag cccccgcccc tgctgggttt
    12961 gcaggtttgg gggcgtgaca gttattgcag gggttcgtga cagttattgc aggggggcgt
    13021 gacagttatt gcaggggttc gtgacagtta gtacgggagt gacgggcact ggctggcaat
    13081 gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac tttccgctaa gcgatagact
    13141 gtatgtaaac acagtattgc aaggacgcgg aacatgcctc atgtggcggc cctcgcgaga
    13201 attaattcag ataaaaaaaa tccttagctt tcgctaagga tgatttctag cgatgaccct
    13261 gctgattggt tcgctgaccg tagactggtt ggcttggttt catcagccat ccgcttgccc
    13321 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
    13381 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
    13441 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
    13501 caaaatcctg tatatcgtgc gacatcgcgc gcgcgcgcgc ggatgctagc ggcgcgccaa
    13561 aaaaaagccc gctcatta
//
