LOCUS       sequence-464767-       16122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 16122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF18-C1878_02750_GsVadh(Gs28C)
FEATURES             Location/Qualifiers
     source          1..16122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      9746..9773
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      9905..9932
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(10364..11215)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(11202..12041)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(12552..13523)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            14762..14849
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      15191..15585
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(15748..15791)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            15887..15996
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatacga
     1501 tggcgctttg ggtccctgtc gctttgtcaa acaacgtcca cccccataga cttggttttg
     1561 actcgtatcg atgctgcggc agggcccggg gatgcgtcga ccgctcgcgc ggccatccgc
     1621 cgctgcggcc aggttcgatt gactcgttct ctagatcctc cccttgtacg ccctaccgat
     1681 gcaccaataa taccgtagaa gtcaatcgct ctagctgcga aatttcctgt gattcgatgg
     1741 gaattcgaac acggccccca ccttcgaaac ctaccccaca tcgagaaagg cgtcatcgga
     1801 ctttagattc ggtattgcac cagaggctcg aacgggcatc agacttttgg tacgagacgg
     1861 cccgttgacc ggcgctgagc gcccgctgac aaattccttt tgtcacgccg aacattttag
     1921 acggctactc gcggcggcct ctcgctcgga caatgaaggc gcatcgaggg gaaaccaaac
     1981 ttcgtgcgtt catcgtttcc aaaacgagcg aaaggagagg aacatgacgg agtggaagag
     2041 gcaagagggc gaggagacca tcttcaggac ctgcgcatgg tcgcccccgg ggtgccaccc
     2101 ggtgggatgc ggtttgcagg tgcacgtcaa agacggcaag gtaacgaagg ttgaaggcga
     2161 ccccgagcac cccatcacca aaggctcgct gtgcacgcgc tgcctagcgc ttccggagta
     2221 catctaccat cccgaccgca tcacgcaccc gctgaagcgc gccaaggaag accgcggcaa
     2281 ggacaagtgg gagcggtgca cgtgggacga ggccaccgac ctcatcgcca agaacgcccg
     2341 cgagatcacc gagaagtacg gcgccgaggc catctgcgtg ttcggcggaa ccggccgcga
     2401 gggcaacaac tggtacccgc agtgggcgaa cctcgtgttc ggcacgccga actccgtgta
     2461 cgcccaggcc ggctggtcgt gctacggccc acgtatgacg aacaccgcct tcatgatcgg
     2521 cggcggctat cccgagatcg actacgccca gaagttcgag gaccgctacg agcacgaggg
     2581 ttgggtcgcc cccgagtgca tcctcatctg gggcaaggag ccgctgcgca gcaaccccga
     2641 cggcctgtac ggccacgccg tcatcgagat ggtgcgccag ttcggcacga agctcatctg
     2701 cgtcgaccca cgtgtgacct ggctcggcac gcgctccgag gaagtgctgc agctgcgccc
     2761 cggcaccgac acggcgctgg ccatggcgtt catctacgtc atggcgcacg acgacctcgt
     2821 ggaccacgac ttcatcgaca agtggtgcta cggctacgag cagctcgtcg agcgtgtaca
     2881 ggacatgccg cccgcgaaag ccgccgagat ctgcggcgtg cccgaagagc agatctggcg
     2941 cgcggcacgg ctgtacggca acgccaagcc cagcagcgtg gcctggggcc tcgccgtgga
     3001 cgagaacccg aacggcaccc agctcggcca gtgcctcatc gcgctcatgg ccatcaccgg
     3061 cttcatcgac gcgcccggcg gcacgacgct cggcatgggc gacgaccagg gcaacgtcgt
     3121 gcgcgacggc ggctccatct ccgccgacga gaaggtggac gacgccaccg actccacgct
     3181 ggagctcgcg ttcaagaacg gcatcatgac cgaggagacg tgggcgaagc gcgtgggcat
     3241 cgacaagtac ccggccatgg gctccatcct gtggacggtc atgcccgacg agttcctgaa
     3301 ggcgcttgag accgagaagc cctacaagat ccatatggcg cacttcatct cgtcgaaccc
     3361 catcggcacc gccatctccg ccgagccgca gcgctggtac gaggcgctca aaaagctcga
     3421 cttcaactgc gcggccgacc tgttcatgaa ccccaccacc atggcctgct gcgacgtgtt
     3481 cctgcccatc gcctccacca tcgagcacga cggcatggtg gtcactcatt acggcctgaa
     3541 ctcgtcgttc tacggcgcgc agaacaagtg cgtgcaggtg ggcgaatgca agtccgacgt
     3601 cgaggccatg atggccgtgg gcaagaagat gtaccccgac ttctggaacc gtttcgagac
     3661 ccagaccgac tacgacaact tccacgtcat gcgctcgtgg ctgccctacg aggagctgcg
     3721 cgagaaggtc accgtcatgt ccaacgagcc gtactacaag tacgagatcg gcaagctgcg
     3781 tcccgacgga caggtgggct tccccaccag cacgggccgc gtggagctgt actcctacat
     3841 gtacgagcag ttcggcgaga accccctgcc ctactacgag gatcccgcct acggccccga
     3901 gcgcacgccc gagctcatgg agaagtaccc cttcatcctc accacgggcg cccgtcgcca
     3961 ggagttcttc cattccgagc acaagcaggt tcccagcctg cgccagatca ccccgtatcc
     4021 gcagatcgac ctcaaccccg ccgatgccgc gcgcctcggc atcgaagagg acgactgggt
     4081 ggagatcgcc tcgccctacg gcgccgtgcg gcagcgcgcc cacgtggtgc ccaccatcaa
     4141 ggagggcgtg tgccacgcca tgcacggctg gtggtacccc gaggaagacg gcgaggaacc
     4201 caacctgttc ggcaactgga agtcgaacat caacgtgctc atgcccaaca gcgtgaacgg
     4261 cgtgatgggc ttcggcaaca cgttcaagca gatgatctgc tcgctgaaga aagtcgaggg
     4321 cgagggaggc ccgaacgatc ccgacaacac cggcatcttc ctggagccca gccgccaggc
     4381 cgagttcacc taccagcagg tatggcgtcc caccgacgaa accaagcagg cttagaaccg
     4441 tttgaaagag aaggaggtac tgaaaatgac gcaaatggcc ctgtacatcg actacgaatt
     4501 ctgctctggt tgccacagct gcgaagtcgc atgccgcaac gaaatcgagt gcggaatcga
     4561 cgattgggga atcaaagtcc tcgaggacaa gccgcgccag cacgctgacg gctcctggca
     4621 ctgggactac ctggcgcttc cgggcgagct gtgcgacatg tgcgaagagc gcgtcgcggc
     4681 gggaaagctt cccgcctgcg tgcagtcctg ccaggccaag tgccttgaga tcggaacgat
     4741 cgaggaatgc gcccaaaagc ttgccgccaa gggcaagaaa gcggcgatcc tcgttcctta
     4801 ggaccgaagc gacacgtcga ttcgaagtcg ggggatgcga agggtccgcg tcgcatcccc
     4861 cctcgcaagg gagatgagcg agattgggca gctgcggagc gttttgctcg ttttgcggaa
     4921 ggtgcggcaa aaccgtcgag aagtctttcg ccaacccgaa accccccgac gtcgccccac
     4981 cgggcgtgtc ggctcgggat gcgaagcggg aagcggaggc ccataagacc gaagggaacg
     5041 tgtcgagggg aaaccaccac atatcgaagg aggggttatc ttgagcgcaa ccacagccac
     5101 actaccgaac caaaccgcca acggcaagca gatgaaaaag ctcatcgcca tcgtcgtggc
     5161 agcgttggtc ttcctgttct tccagttcgt ttgcccggtg ccgaccggcc tggagagaac
     5221 cgccatgtcg gcggtcggca tcctgctcgc ctgcatcatc ctatgggtca ccgaagccct
     5281 gccgttcatc gtcacggtcg tgctgatatt cctgctgata cccctaacgg gaatcttgcc
     5341 ctacgcgtcg accgaaaccc aggcgagcgt gttcaattcg tccagcctgc tcgtgcccat
     5401 ctactgcctg ttcgtgttca cggtgtccgg cgccgtcatg tccacgccga ttccgtatcg
     5461 cgtcgccagc gcggccctga agttcgcggg caacagcacg acgaagctca tcatcggctt
     5521 ctcggtggcc acgtcggtcc tgtcgatgtt cgtctccgac ctcgccgcct ccgccatctt
     5581 catcggcatc ggcctgtcca tcgtcgaggc gaacggcgga atcaagggca aatccggtct
     5641 cgccaaagcg ctcacggtgt ccgttgccgc ggcggccgcc atcggcggca tcggcacgcc
     5701 aatcggcaac tcgctgaaca tcctgtgcat gaccttggtc gaaacctaca tgggggtgcg
     5761 cgtcacgttc ttggcctggt gcgccgtcaa catcccgctg gccttggtgg tgtccgtcat
     5821 ttccggcttc tggtgctcca aggtgttcaa gatcgaggag atcaaaccgg aggccctgtc
     5881 ggtcgtgtcc gccaagctcg ccgattacgg caagatgacc gtcatcgaga tcaagctcat
     5941 cgtctggttc gtcatcgcct tcggcctgat gatcatgagc acgtggttcc cgcagatcaa
     6001 ctccatgctc gtcgccttcg ccttcacggt tatcgctttc gtccccggca tcaacctgct
     6061 gagcaaagag cagtactaca agaacatccc ctgggagatc atcatgatga tcatcggcgt
     6121 gcaggccctg gccaccggcc tggtgggcac cggcgtcgcc gcatggttcg tcaacaccgt
     6181 gcttgccggc gcatccgctt ggccgctgct gctcgtcgtg ctcgtcatgt gcttcatcac
     6241 gatgatcctg cacatctgca tccccgtcgg cccgcccacg gtgtccgtcg ccgtgccgct
     6301 catgatcgcg ctggccgcca ccaccggcgt gaacggcgcg gtcatcgcct gtatcggcgg
     6361 catcctcggc ggcgtcacca cctggcttcc catcgactcc atcatgatga tctgctacga
     6421 gaagggctgg atcagcatgg cggagtggct gaagaaggca tggttcccca ccgtcgtgct
     6481 ctgcgtgctg agcgccctgt acttgcccgc catgaccggg ctcatgggct actagtttcg
     6541 tttccgacca tgccgggcgg ccgcggccgc ccggcagcca agctgtcgcg ttttatgcat
     6601 ctaaggagga ggcaatggga gaccccggcc ttgtcggcgc gatcgtcgtc ggcatcgttt
     6661 ttctgatgct ggcagcggcc gtcggtaaaa agaagaacaa gaacaagacg aaagaccagc
     6721 ggttgagcga gtgcttcgag gaatacgacc ggaagatgga caagttccgt cgctagcctc
     6781 cccaacgccg cttcacccca aagagaagga gaaccaccat gtgcttcaga ccagcgacca
     6841 cctccacgcc cgcccccgga gccggagcgc ccggcgcccc ctcggccccc ggtgcaccgg
     6901 gtgcaccagg cgcacccggc gcaccaggcg cacccggtgc gcctgcggcc cccagcgcac
     6961 ccggcactcc cgcggcgccc ggaaaccccg gcaccgccgg caaatccgcc gcaacggacg
     7021 cgcccggcga ataaccggcc attcgcgacc gttcgacttc aaggagaggg ctcaccggtg
     7081 actaccctgc agataatttc gatcgtggtg ttcgtggccg tcatggccct ggtcgtatcg
     7141 gagaagatac accgagcgct cgcggcgctg ctcggcgccg tcgtgctgtt ctgcatcggc
     7201 gtcgtcgact tcgacgcggg catggagtcg atagacttca acacgctcgg cgtcctgtgc
     7261 ggcatgatga tgttcgtggc cgtggtgaag cagtccggcc ttttcgaata cgtggccata
     7321 cggtcggcca agctgtgcaa gggaaacccc tggaagatca tggtcgcctt cgtggtcatc
     7381 acggcggtgt tctccgccct gctcgacaac gtgaccacca tcctgctcat cggccccatg
     7441 accatcatgc tgtgccgaac gctgaagatc gacgcgaccc ccttcctcat cgccgagatc
     7501 atcgcgtcga acgtaggggg gaccgccacg ctcatcggcg acccgccgaa catcatgatc
     7561 ggttcggcgg cgggcctcac cttcttcgac ttcatccgct acgacgcccc cgtcgtggtg
     7621 gtcatcctcg tcgtgctgct gctcgtgttc aggatgctgt acggacgcaa gctgttcgtc
     7681 gacgagggcg acatgttcaa ggtgatggcc ctctccgaac aagacgccat caaggaccgc
     7741 cccctgttcg tcaagagcat cgcgatgatc gtggccgtca ccgccgcgtt catgctccac
     7801 ggcgtgctgc acctcgagtc ggcggtgatc gccttgggtg ccgccggaat catgctcctc
     7861 atatcgagaa gcgatctgga agtggcgctc gagggcgtcg aatggggcac catcggcttc
     7921 ttcctcggcc tgttcgtggt ggtgggggcc atggagcaca ccggcgtcat cgcgcttatc
     7981 ggcgaggggc tcgtgtcgct caccgggggc aacgtggtgc tgctcatgat cgccctgctg
     8041 tggggctccg cgctgctctc ggccgtgctc gacaacatcc cgttcgtcgc caccatgatc
     8101 cccatcatca ccgtcatggc atcgacgggc atcgacgtgt tccccctgtg gtgggccatc
     8161 tcgctcggcg cctgcctcgg cggcaacggg tcgctcgtgg gagcgtcggc caacgtcgtg
     8221 ctgtcgggca tcgcgaacaa agagggctac cccatcagct tcatgggctt caccaagatc
     8281 ggcttcccca tcatgctgct gacggttgcc atcgcaacgg cgtacctgct cgtggtcttc
     8341 tagccgacgg cggcaggcgc gacgcgaagt ggaaggacgg cccgtcgggc tcgaacccga
     8401 cgggccgtcc ttcatgcgcg cccgcaggac gcagctacgt tgcgagcccc gcgaaagcgg
     8461 gcgcggcgtt ccttgaaggc ctagaaggtg ccttggatgc cttgggcgaa cttcgctatc
     8521 gagcggaagc tcgccgagtt tttctggaac agtatgtaga agtgccaatt caggcgagga
     8581 tccgacagag ggatcgcgca gaaccccgca ggaggggcca cggagaactt gtcggagacg
     8641 atgcccgtgc cgccgctcct cttcaccaca tggatcatcg agctcgtgct gggtatgacg
     8701 ctgacgttgg cccgatcgaa gccgagcgag tcgagttgcg cgaacagcgg cgcgtactgg
     8761 aaaaccggct ccgacatcag cagcagcttc tggtccttca agtccatcac cgacaggctc
     8821 tccttgcggc ccaacggcga tgcggcgctc gtcagcgcga acgaaaccga cccctccccc
     8881 atctcgagca cgtcgcaatc ggggaacttc cgctccatgc acataacgac ggcgacgtcg
     8941 gccttccccg tgcaaaccga tcgatagcaa tcctcgcagc tcatctcgga cacccgcagc
     9001 cggtcggaat gctgcgcgat gtactcgtcg gtacccagcg atatccctgc gaagaggttg
     9061 gtgctcaccg cgaggttgag cgtttggtcg gatgcgtcgg ggccggcgaa catgacgtgc
     9121 aagccgtcca ccttcgtgac gatctcgcgc gcctcgccgg ccagctgctg ccccgcggga
     9181 gtgagcacca tctcgttgcc atggcgctcg aagagcgcga tgccgagctc ggtctcgatg
     9241 gccttgatgg agtgcgcgac gttctgcctg ctggtgaagc accgtttggc cgcctgggcg
     9301 taactggagt tctctgccgc agccagcaga tgacgaagtt ggtggatttc catacccgac
     9361 cccctgcccc gctcgttgtc gacggcaagt ccgcggcacc ctgaaacccg cgacgccgtc
     9421 aacctccaag gaaaacgaat ccctccggca aaacgcattc cgcttcgccg tccccatggt
     9481 tcgattataa ccgattatca tgaagcctca acgagagaga cgaaccggca ttgttccgag
     9541 gagtcgcctt cgcatatccg aaatccgccg gctcggctca cgcccgggaa gcgcacttca
     9601 gatatgggct cgatatcgcc ggaattcaga tcaatgcgtc gaatgccgca ccggcaacgt
     9661 cgtttggaac tgacggtgcg atgaaatcga cctgggtttg ccttatcctt tctttagagg
     9721 cgaatcggac tagtcgactc acactggctc accttcgggt gggcctttct gcgtttatat
     9781 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta tttaattcct
     9841 gttataaaaa aaggatcaat tttgaactct ctcccaaagt tgatccctta acgatccctg
     9901 caggagaagg ccatcctgac ggatggcctt tttgaagctt tctagaatcg ggggcccccg
     9961 gggggattct agaacaatgg aagtcctcgt ctcgccctcg aattagcccg cttaatgagc
    10021 gggctttttt tgaattaatt ctcgcgagct ggcacgacag gtttcccgac tggaaagcgg
    10081 gcagtgagcg caacgcaatt aatgtaagtt agcgcgaatt gcaagctggc cgacgcgctg
    10141 ggctacgtct tgctggcgtt cgggagcaga agagcataca tctggaagca aagccaggaa
    10201 agcggcctat ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat attgttaagc
    10261 cttttctgag catggtattt ttcatggtat taccaattag caggaaaata agccattgaa
    10321 tataaaagat aaaaatgtct tgtttacaat agagtggggg gggtcagcct gccgccttgg
    10381 gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca
    10441 gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct
    10501 ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt
    10561 cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca
    10621 cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg
    10681 ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc
    10741 gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga
    10801 tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg
    10861 ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg
    10921 gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta
    10981 ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca
    11041 gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca
    11101 gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt
    11161 gcgccgggtc gtgtcggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc
    11221 acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc
    11281 cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg
    11341 acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg
    11401 ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc
    11461 tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg
    11521 atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg
    11581 ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg
    11641 cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg
    11701 ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg
    11761 aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc
    11821 aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca
    11881 atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc
    11941 gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct
    12001 gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg
    12061 cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt
    12121 ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca
    12181 tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca
    12241 gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg
    12301 gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg
    12361 gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca
    12421 ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt
    12481 tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac
    12541 caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt
    12601 gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg
    12661 ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg
    12721 ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc
    12781 tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc
    12841 atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcggac
    12901 agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc
    12961 tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc
    13021 agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag
    13081 cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg
    13141 gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc
    13201 catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct
    13261 ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc
    13321 tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc
    13381 gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg
    13441 tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc
    13501 tgcaaagtcc tgtcgttctt catcgggcca ccaagcgcag ccagatcgag ccgtcctcgg
    13561 ttgtcagtgg cgtcaggtcg agcaagagca acgatgcgat cagcagcacc accgtaggca
    13621 tcatggaagc cagcatcacg gttagccata gcttccagtg ccacccccgc gacgcgctcc
    13681 gggcgctctg cgcggcgctg ctcacctcgg cggctacctc ccgcaactct ttggccagct
    13741 ccacccatgc cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc tgcgcctcgc
    13801 tggcctgctg ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc gccatgctct
    13861 gggccagcgg ttcgatctgc tccgctaact cgttgatgcc tctggatttc ttcactctgt
    13921 cgattgcgtt catggtctat tgcctcccgg tattcctgta agtcgatgat ctgggcgttg
    13981 gcggtgtcga tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg gccttccatc
    14041 tccaccacgt tcggccccag gtgaacaccg ggcaggcgct cgatgccctg cgcctcaagt
    14101 gttctgtggt caatgcgggc gtcgtggcct gcccgctcta atgcccggtt ggcatggtcg
    14161 gcccatgcct cgcgggtctg ctcaagccat gccttgggct tgagcgcttc ggtcttctgt
    14221 gccccgccct tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc
    14281 tcgatgccgt cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca
    14341 ccggcatgga tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac
    14401 tcggacgcca gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc
    14461 tccttgaaca gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg
    14521 ggccgctcga cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg
    14581 cgggcatact tgccttcgcg ctggatgaag tcggccttgg ccctggccga ttggccgccc
    14641 gacctgctgc cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt
    14701 tgcttttcgg ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag
    14761 gccagtttct cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt
    14821 gccgccgctg tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga
    14881 tggttaaggg gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc
    14941 gaatcaatgc cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg
    15001 aaacaaggcg caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt
    15061 ggccggagga tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg
    15121 ccttgttcgg tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca
    15181 ggccctgcgg ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc
    15241 ggctaaaagc gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc
    15301 cgcctttccc cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc
    15361 agctatccgg cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct
    15421 gataaccgcg cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg
    15481 ccagcccccg cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc
    15541 gtgacagtta ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg
    15601 gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa
    15661 gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg
    15721 cctcatgtgg cggccctcgc gagaattaat tcagataaaa aaaatcctta gctttcgcta
    15781 aggatgattt ctagcgatga ccctgctgat tggttcgctg accgtagact ggttggcttg
    15841 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
    15901 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
    15961 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
    16021 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgacatc gcgcgcgcgc
    16081 gcgcggatgc tagcggcgcg ccaaaaaaaa gcccgctcat ta
//
