LOCUS       sequence-464772-       14172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Cadh without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14172)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 14172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN3-Elen0507-Cadh-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..14172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      7796..7823
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7955..7982
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(8414..9265)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9252..10091)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10602..11573)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12812..12899
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      13241..13635
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13798..13841)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13937..14046
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcat
     1501 gccttcttct gctcgtcgat ggtctgctcg accagattga ggatctcctg ctggttgtgg
     1561 acgtccatct tcgcatagat gttgcgaagg tgggtcttcg cggtggactg cgtgatgacg
     1621 agcgtgtccg cgatatgctg cgcggtgcgg ccgcgtgcca gcaatacgag gatctcctcc
     1681 tcgcgccccg tcagctggta ctggtcggtg agcgcatggc acgcgttctg cagcttgttc
     1741 gcgtggggaa gcgcatcgtg aacgtcgggt gcgtcagggc cgggccattg accgcggatt
     1801 ggcgtgtggt agtcggtgat gcttcccgaa agcgacgtga tggctccgca ggcgaacaat
     1861 acgacaagcg cgatgaccga gctggacacg aatacgtcgg gcgacatatg ctccaataga
     1921 agagagctcg ccgctttgcc cgcggtgaac gagaggaacg cgacgcacca gggcatcagg
     1981 tagggtcgtt tgctttcgat gaggaagggg gccagcatga taaagaggaa caccagcttc
     2041 tgcgtgatgt tcagcaagac gaggtagagg aatatccacg tctcgttcac gaaggacgac
     2101 agccacaagg caagaagcac ggtgggtatg aggaacacct tgtacagctc gaggctccgc
     2161 ctgttccaga agtactggac gagcacgagg atggccgctc cgccgcatgc ggtgcccacg
     2221 gccgtgccca gctggatgat gagcgatacc gtcccgccgt cttgcagggg aatccattgg
     2281 tagtggatat gtccgaacac cacggcatag caagccagcg acagcatcat cgtcacgacg
     2341 aatactcgtc gaccgcgagg agcctggtcc gaggggacga tgagggatcg atcggggcag
     2401 ccgattcccg ccaccatgct ccgctccggg ctcgcgcagg catcctcgct cgggatgtcg
     2461 tcgcgatccg cacgcggcgc gcgcgaacgg gcgtattccc tgaaatagca gagcagcacc
     2521 atcgacacga tggggagcag cgataccgtg ccgtaagcgg catccacctt cagaaacgcg
     2581 atgaggctgt tcattgccgc aacgacgatc gttcccaggc ccagcacggc ggcgcaggcg
     2641 cgcgccccgt agggaaagag cgctttcatc cagagaaaca ggatgacgcc ctctccaagc
     2701 ccgaatacga tgcgcgcgat ggaggatacg atggggttgt cctcccatac gctttgctgc
     2761 aagacgacga acatggaagc cgacatcaac gctcctgcgc cgatgacgat cgcggcggga
     2821 acgtgggtgg gcgcgtccag cttgcgatac gcaagagcca gcatgatcag gagaaggaac
     2881 gatacgagcg acgcgacggc gtggtagtcg ccgctgatat tgacggttct gatcgtcgag
     2941 aggcggtcgc cttcgatggc gagcagcgcg catccgaagc cgatcgaagc gatcgcgatt
     3001 cccggcacct cgcgaatcgc cagcgcaagc ggcgacgatc ctcgttcgtc tgtcatgcgg
     3061 accccctttt actcacatcc ccatggcgat aaggctcgtt cgagcattgt cgtcagtata
     3121 gcaattcgcc gataccgcgg cggcgggcgg atggcgaaat accactctcg gtggtaggtc
     3181 tttcatgagc tcgatagtag ggtttctgac ctggttgaac agagttcgca tcagcggtgg
     3241 ggtgctcaac tcgcgctcga tctgcatcat ggttcgcacg gacgcttggt ccggcccgct
     3301 cgttggagtg cgggtgaaaa gggaaccggc agaggaggag ctatggcgaa caccacgcaa
     3361 aggcggtcgt tcggcaccat gagccgtcgc agcttcatga ggctcgcagg ggtgacgagc
     3421 gctgcgcttg cgctgacgtc ggcaacggcg ccggcggcgc ttgccgaaga gcacgacagc
     3481 ggaacggttg cgtcgtcgga cggcgtgcag cgtattcgca cgatgtgccg cgggtgcggc
     3541 aagatggaat gcggcgtgtg ggtgacggtg gagaacggcc gcgccatcaa gatcgagggg
     3601 gacgagagct cgttcgcgtc gtcggggaac agctgcagca agtcgcaggc ttcgctgcag
     3661 gcgtgctacc atcccgatcg gctcgcgtat cccatgaaac gcacgaatcc caagggcgac
     3721 gacgatccgg gttgggtgcg catcagctgg gacgaggctc tcgccgaagc cggcacgaag
     3781 ctcaacgaga tcaaggagca gcgcggcggg aactccatgt tctcgatgtg cggcaccagc
     3841 cgcatctact gcatggcgag cgcgctcggc atgcagggca ttctgaacac ggcgaacacc
     3901 catcaggcgt accagatttg caagggtccc cgccatgtgg ccaccggtat ggtgtcggct
     3961 cgtgcgtaca gctggatggc cacggtcgac cggccgagcg tgttcgtgca atggggcggc
     4021 gcttcggagc tgtccaacta cgatgactcg tgtcgcacga cggtcgatgc tgcggtcaag
     4081 gcggacaagc acatcatcgt cgatccgcgt cagacgaacc tcggcaaaga agccgacata
     4141 tggaatccgc tgcgtcccgg caccgacggc gcggtggggc tcggctggct caacgtgatc
     4201 atggagtaca acctgtacga cgagctttgg gtgaagcggt ggacgaacgg cccgttcctc
     4261 gtgtgcgagg atatcgagcc ttccggttgg cagcagatgg gtgccggcgg tccagaagaa
     4321 atcaaaacgc gcctgctcaa ggaatccgat gtgcaggaag acggcagtcc gaaacggttc
     4381 atggtctacg atcagttgaa ccagcggctt acgtatttcg atgcggatac gggatactgg
     4441 gagggcgagc agccgcgtac gctgacgggg aaggaggcgc ggcaaaagca cctcgcaccc
     4501 ggcgtgacgc aaggttgggt gcccgatcct accgggttcg atccggagat cgatcctcag
     4561 atattcgggc aggtggaagt caccctgaaa gacgcttcga cctcggtctg caaaacggtg
     4621 tggcaaacgt tctcggacta cgtagccgat ttcacgcccg agaaagtgga ggagatcacc
     4681 agcgtttcgg ctgatgcttt gcgcgaggcg gccatcacct atgcgacgcc tatcgatcca
     4741 tcgaccggat acggcaacgg cggcatccag tacatgctgg ccatcgagca tgcgtgcaac
     4801 tcggtccaga acagccgtat ctgcgatctc atcgtgggga tcacggggaa cttcgacacc
     4861 cccggcggca atcgcggcgc aacggccgcg actttcgacg aagagttcgc catgatgggc
     4921 agcggtctgc ccatggcgtc ggctgacttg tgggacaagg tgctgggcgt ggaggacatt
     4981 cctttgctca agcatcacgg catctgggcc gattcgacgg ccatttggga tgcgtgcaac
     5041 aacgaagggg cgccgtaccc gttgtacggc ggcgtctgcc agtcgggcga tgtgatgaac
     5101 atgtccaacg ctctttgggg ttgggaaggc ttgaagaagc tggacttcct gctggacatc
     5161 gacctgtggc atacgcccac gtcgcagctg gcggatatcc tgctgcctgc gcgccattgg
     5221 ctcgaggtgg attgccctcg ccgctcccaa gggtctggcg gcatggaggg aagccattgc
     5281 aagtgcgtgg agccgctcgg cgagagctgg ttcgacgtgg acatcatcat ccagctgtgc
     5341 aaggccatgg gcattccctg gagcgccgac cccgacgatc cgtggccgga ctccatcaag
     5401 gagcttgatg cggcatgcga gccgatgggt cttacctggg aggagtggaa gcaggagttt
     5461 cagaagaccg gtttccgcga ctgcaagaag gaataccccg acgactgggg cacttaccga
     5521 cgctacgaaa cgggccactg ccgctccgat ggcaagccgg gcttgcagac gcccacgctc
     5581 aagcaggaga tatggtcgac catcatcgag acgtaccatc ctgacggccg gtacaacctt
     5641 cccacgtatt ccgagccccc ggagagtccc gttgcgcagc ctgagcgggc tcaggagtac
     5701 ccctacatca tgacgacggg ccgtcgcatt cccgtgtact tccactccga gcaccggcag
     5761 ctgccgtggt gccgcgagct gtggccggtg ccgcgcgtag agatcaaccc gaaggatgcg
     5821 cttgagctcg gcatagagca gggcgattgg gtgtggatcg agacagagcg cggcaaggtg
     5881 cgacaagtgg cggatctcta tcacggcatc cgcccgggga ccatcaactg cgagcatcag
     5941 tggtggctgc ccgagttcca cggcgcgacg aagggtttcg acctcatcag catcaactgc
     6001 ctggtgaaca aggacatgcg cgatcctctg tgcggatctt cgtacgcgcg cgcttacaac
     6061 gtgaaggtgt acaaagccac gcccgagaac tcgccgttcg gcaatcccgt gccgtgcgac
     6121 gtcgacggga ccgagatgat cacgtcgccc gatgacccgc gtttgaagga atggctgccg
     6181 aactacgagg ggagggacta ggctatgact cagtatgcga ttgccaccga tctcaatcgg
     6241 tgcgtaggct gcctggcatg ctcggtgacc tgcaaagtgg tgaacgacgt tcctatcggg
     6301 tcgtattgga acaaggtgct gcgcatcggc ccgaatccga agttcgaagg cgccgagttc
     6361 cccgatgtgg agatgtactt cctcaccgtg caatgccagc actgcgccga ccccgagtgc
     6421 gtgaaggtgt gcccgaccgg tgcctcgcac aagctggagg acggaaccgt gcagatcgac
     6481 aagtccaagt gcatcggctg ccagttctgc gccatgtcgt gcccgtacgg ggtgcggtat
     6541 ttgaacgagg aggagcgcgt ggtggagaag tgcaccttgt gcgagcagaa gattgcccag
     6601 ggcgagctgc cgcagtgcgt cacgcaatgt ggcggccgtg cgcgctattt cggtgatctg
     6661 gagcaaggga tcgcaggttt cgagggcccg gctcccatcg atatcgcttc aaggaacgtc
     6721 gactacgatg cgaccgtcca gagtcgcgcg aagttggtcg acgagatcga gtcgtatgcc
     6781 gaaagcgatg tgtatcgcct gccgaacgtg gggaacaacc cgagcttcgt gtacatcctg
     6841 cgcaatcgta cgtggcaagg aggcggtgaa taatggaact gcagtggcct ttgattatct
     6901 ttaccaccct ggttgcctgg tcggcggggc tgttcgggac gcaggcgctg atggcggtgt
     6961 tcggcgtcgg caagaaggcg caggtgccgg cttgggtttg ctcggccgtc ttgctcgcgg
     7021 ccggcgggat cgcggtgttc ttccacctgg agcactggga gcggatcttc aacgggttcg
     7081 ggcacctgac gagcgggatc acgcaggagc tgatcgcgat cgtcgtgctg gcggtcgtcg
     7141 cggtcgcgta tctcgtgctc atgcgcaagt ccgacgacgg cgcgagcgtg cccaagtggc
     7201 tggcgtggct gtccgtcgcg ctgtcggtgg cgctcgtggc cgtcatggcg cactcgtaca
     7261 ccatggccgc gaggccggcg tgggactcgg tcctctggat cctctacgtc ctcggcaacg
     7321 cctgcgtgtt ggggccggcg acgttcctcc tcctctccgc cttggctgca gggggacccg
     7381 gggaccagcc cgccgagcgc gcagccggcg ccggggcccc cgcgggtctc gcggccctcg
     7441 ccggcgccgc cctcaacgcc ctcgcggccc tcgccttcgc cgccttcctc cagctctccg
     7501 ccggctcctt cgccgacgtc ggcctctact tcgacccgac gcaccccacc agggccatgg
     7561 ccgacgccgc ggccaccgtg ggcgcgcagg ccccgctcct gtggctggga gtggtggccg
     7621 tcggcgcgat cgtccccctg gccgccgcct tcgtgggcag gcgcaccggc aactggaagc
     7681 tctgggcccc ggtcgccatc gccgccgccc tcgtcggagc cgtctgcatg cgcgtcgcct
     7741 tctacaacct ggggctctcg gtcttcatgt tctactagac tagtcgactc acactggctc
     7801 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     7861 ttaatccggc ttttttatta tttaattcct gttataaaaa aaggatcaat tttgaactct
     7921 ctcccaaagt tgatccctta acgatccctg caggagaagg ccatcctgac ggatggcctt
     7981 tttgaagctt tctagaatcg ggggcccccg gggggattct agaacaatgg aagtcctcgt
     8041 ctcgccctcg aattagcccg cttaatgagc gggctttttt tgaattaatt ctcgcgagct
     8101 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
     8161 agcgcgaatt gcaagctggc cgacgcgctg ggctacgtct tgctggcgtt cgggagcaga
     8221 agagcataca tctggaagca aagccaggaa agcggcctat ggagctgtgc ggcagcgctc
     8281 agtaggcaat ttttcaaaat attgttaagc cttttctgag catggtattt ttcatggtat
     8341 taccaattag caggaaaata agccattgaa tataaaagat aaaaatgtct tgtttacaat
     8401 agagtggggg gggtcagcct gccgccttgg gccgggtgat gtcgtacttg cccgccgcga
     8461 actcggttac cgtccagccc agcgcgacca gctccggcaa cgcctcgcgc acccgctggc
     8521 ggcgcttgcg catggtcgaa ccactggcct ctgacggcca gacatagccg cacaaggtat
     8581 ctatggaagc cttgccggtt ttgccggggt cgatccagcc acacagccgc tggtgcagca
     8641 ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat gctgatgcgc acatgctggc
     8701 cgccacccat gacggcctgc gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg
     8761 cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg ccgcttgcgg ccattctggg
     8821 cgatgatgga taccttccaa aggcgctcga tgcagtcctg tatgtgcttg agcgccccac
     8881 cactatcgac ctctgccccg atttcctttg ccagcgcccg atagctacct ttgaccacca
     8941 tggcatcagc ggtgacggcc tcccacttgg gttccaggaa cagccggagc tgccgtccgc
     9001 cttcggtctt gggttccggg ccaagcacta ggccattagg cccagccatg gccaccagcc
     9061 cttgcaggat gcgcagatca tcagcgccca gcggctccgg gccgctgaac tcgatccgct
     9121 tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga
     9181 gggcacggaa caggccgggg gccagacagt gcgccgggtc gtgtcggacg tggctgaggc
     9241 tgtgcttgtt cttaggcttc accacggggc acccccttgc tcttgcgctg cctctccagc
     9301 acggcgggct tgagcacccc gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg
     9361 ccatagttgg ccttgctcac accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca
     9421 ccccattcct cggcctcggc gctggtcatg ctcgacaggt aggactgcca gcggatgtta
     9481 tcgaccagta ccgagctgcc ccggctggcc tgctgctggt cgcctgcgcc catcatggcc
     9541 gcgcccttgc tggcatggtg caggaacacg atagagcacc cggtatcggc ggcgatggcc
     9601 tccatgcgac cgatgacctg ggccatgggg ccgctggcgt tttcttcctc gatgtggaac
     9661 cggcgcagcg tgtccagcac catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg
     9721 aaccactccg gggccatgat gttgggcagg ctgccgatca gcggctggat cagcaggccg
     9781 tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc caagggcgtg caggcggtga
     9841 tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca ccgggccggt gggcagttcg
     9901 cccacctcca gcagatccgg cccgcctgca atctgtgcgg ccagttgcag ggccagcatg
     9961 gatttaccgg caccaccggg cgacaccagc gccccgaccg taccggccac catgttgggc
    10021 aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct ccagaatatt gataggctta
    10081 tgggtagcca ttgattgcct cctttgcagg cagttggtgg ttaggcgctg gcggggtcac
    10141 tacccccgcc ctgcgccgct ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt
    10201 cgtcggtcag ccagaacttg cgctgacgca tccctttggc cttcatgcgc tcggcatatc
    10261 gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc ggtctgcttg tccttttggt
    10321 ctttcatatc agtcaccgag aaacttgccg gggccgaaag gcttgtcttc gcggaacaag
    10381 gacaaggtgc agccgtcaag gttaaggctg gccatatcag cgactgaaaa gcggccagcc
    10441 tcggccttgt ttgacgtata accaaagcca ccgggcaacc aatagccctt gtcacttttg
    10501 atcaggtaga ccgaccctga agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt
    10561 gagtactcat agtataacag gcgtgagtac caacgcaagc actacatgct gaaatctggc
    10621 ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc gtgccagctc ggcccgcgca
    10681 agctggacgc tgggcagacc catgaccttg ctgacggtgc gctcgatgta atccgcttcg
    10741 tggccgggct tgcgctctgc cagcgctggg ctggcctcgg ccatggcctt gccgatttcc
    10801 tcggcactgc ggccccggct ggccagcttc tgcgcggcga taaagtcgca cttgctgagg
    10861 tcatcaccga agcgcttgac cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg
    10921 cgccggtggc ggctaagctg ccgctcggac agttcgaggc tggccagcct gcgggccttc
    10981 tcctgctgcc gctgggcctg ctcgatctgc tggccagcct gctgcaccag cgccgggcca
    11041 gcggtggcgg tcttgccctt ggattcacgc agcagcaccc acggctgata accggcgcgg
    11101 gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc ggccatagtg gcggctgtcg
    11161 gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc gggcaatctg cccccgaagt
    11221 tcaccgcctg cggcgtcggc caccttgacc catgcctgat agttcttcgg gctggtttcc
    11281 actaccaggg caggctcccg gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg
    11341 tcgtccacca gcaccagacc atgccgctcc tgctcggcgg gcctgatata cacgtcattg
    11401 ccctgggcat tcatccgctt gagccatggc gtgttctgga gcacttcggc ggctgaccat
    11461 tcccggttca tcatctggcc ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag
    11521 cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca
    11581 ccaagcgcag ccagatcgag ccgtcctcgg ttgtcagtgg cgtcaggtcg agcaagagca
    11641 acgatgcgat cagcagcacc accgtaggca tcatggaagc cagcatcacg gttagccata
    11701 gcttccagtg ccacccccgc gacgcgctcc gggcgctctg cgcggcgctg ctcacctcgg
    11761 cggctacctc ccgcaactct ttggccagct ccacccatgc cgcccctgtc tggcgctggg
    11821 ctttcagcca ctccgccgcc tgcgcctcgc tggcctgctg ggtctggctc atgacctgcc
    11881 gggcttcgtc ggccagtgtc gccatgctct gggccagcgg ttcgatctgc tccgctaact
    11941 cgttgatgcc tctggatttc ttcactctgt cgattgcgtt catggtctat tgcctcccgg
    12001 tattcctgta agtcgatgat ctgggcgttg gcggtgtcga tgttcagggc cacgtctgcc
    12061 cggtcggtgc ggatgccccg gccttccatc tccaccacgt tcggccccag gtgaacaccg
    12121 ggcaggcgct cgatgccctg cgcctcaagt gttctgtggt caatgcgggc gtcgtggcct
    12181 gcccgctcta atgcccggtt ggcatggtcg gcccatgcct cgcgggtctg ctcaagccat
    12241 gccttgggct tgagcgcttc ggtcttctgt gccccgccct tctccggggt cttgccgttg
    12301 taccgcttga accactgagc ggcgggccgc tcgatgccgt cattgatccg ctcggagatc
    12361 atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga tggccagcgt atacggcagg
    12421 cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca gcgccttctg ctggtcgagg
    12481 gtcagctcga ccggcagggc aaattcgacc tccttgaaca gccgcccatt ggcgcgttca
    12541 tacaggtcgg cagcatccca gtagtcggcg ggccgctcga cgaactccgg catgtgcccg
    12601 gattcggcgt gcaagacttc atccatgtcg cgggcatact tgccttcgcg ctggatgaag
    12661 tcggccttgg ccctggccga ttggccgccc gacctgctgc cggttttcgc cgtaaggtga
    12721 taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg ctccatgcaa tggccctcgg
    12781 agagcgcacc gcccgaaggg tggccgttag gccagtttct cgaagagaaa ccggtaagtg
    12841 cgccctcccc tacaaagtag ggtcgggatt gccgccgctg tgcctccatg atagcctacg
    12901 agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg gagcaacaag gcggcggatc
    12961 ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc cgaaattcag cgggtgcggg
    13021 caagggaaca gcagcaagag cgcaagaacg aaacaaggcg caaggtgctg gtgggggcca
    13081 tgattttggc caaggtgaac agcagcgagt ggccggagga tcggctcatg gcggcaatgg
    13141 atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg tctgccgcca cgccagaagg
    13201 atgagccggg ctgaatgatc gaccgagaca ggccctgcgg ggctgcacac gcgcccccac
    13261 ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc gctccagcgt atttctgcgg
    13321 ggtttggtgt ggggtttagc gggctttgcc cgcctttccc cctgccgcgc agcggtgggg
    13381 cggtgtgtag cctagcgcag cgaatagacc agctatccgg cctctggccg ggcatattgg
    13441 gcaagggcag cagcgcccca caagggcgct gataaccgcg cctagtggat tattcttaga
    13501 taatcatgga tggatttttc caacaccccg ccagcccccg cccctgctgg gtttgcaggt
    13561 ttgggggcgt gacagttatt gcaggggttc gtgacagtta ttgcaggggg gcgtgacagt
    13621 tattgcaggg gttcgtgaca gttagtacgg gagtgacggg cactggctgg caatgtctag
    13681 caacggcagg catttcggct gagggtaaaa gaactttccg ctaagcgata gactgtatgt
    13741 aaacacagta ttgcaaggac gcggaacatg cctcatgtgg cggccctcgc gagaattaat
    13801 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctagcgatga ccctgctgat
    13861 tggttcgctg accgtagact ggttggcttg gtttcatcag ccatccgctt gccctcatct
    13921 gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg
    13981 tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc
    14041 ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat
    14101 cctgtatatc gtgcgacatc gcgcgcgcgc gcgcggatgc tagcggcgcg ccaaaaaaaa
    14161 gcccgctcat ta
//
