LOCUS       sequence-464773-       14132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 eCadh without accessory genes 
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14132)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 14132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN5-Elen0515-eCadh-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..14132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      7756..7783
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      7915..7942
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(8374..9225)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9212..10051)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(10562..11533)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            12772..12859
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      13201..13595
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(13758..13801)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            13897..14006
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcag
     1501 tcgatttctt gcacgaggtt gatcagctct tgctgggagt gaatccccag cttttggtag
     1561 atgtgctggc gatgggtgga aaccgtgctc ttcgacaggc atagctggtc ttgcacgtac
     1621 ggcaggttgc gtcccttcgc gaacatgatg agaatctcgg tttcgcgttc ggtcagaccg
     1681 taccgatcga ccaccttcag gcatttttcc tggaagcggc gcttgcgttc aagcggaatg
     1741 atgtccatcg ctttcagcag atccgattcg gtgaacacgg cgctgctcac cagcagaatg
     1801 accaaagccg aaaccagcat gatgggaaaa gctgaagcaa gctggatgcc gtgctggtga
     1861 agcacgaacc ggcccagcag catgcccagc agcggtccga ccgcccatgc ggcacgcgcg
     1921 aatgaaaacg tgcgtatcct gaccgacgcg tatcgattgc atacgcccgt cacgatgacc
     1981 cacataaaca agctgaagca ttggtaggcg gccgcattcg cggcgtaggg aaggtaggcc
     2041 gacgaatcgg ggtatacgag aggcagcggc agcagaagcg cgccgatgac gaggaacacg
     2101 acgatcgcgt ggtagcagtt tttcgccatg cgctcggatc ttgagttgag aaggatgagc
     2161 gcgatggcga tcgctccggc ggtcactccg aatgcgacaa gggcctgggt gaacgcgtag
     2221 acggtgcctc cgacgctcgc gatgcccatt tgtatgtaga gggttcggac ggcctcgttc
     2281 gcgaatccca ccaaaagggc gcacagcccg agcctcgtga cgaggtgggc ccagtcgcgt
     2341 cgctcgagcg ggtcttcgat gggcgtctgc tggtcgagcg ggcatggcgc ctcctgcggc
     2401 gccaacccga tgttcttcat gccgaactcg agcaatacgg ctgcgatcag gggcatgctg
     2461 gcggcaaaca cgcatgcctc gaaaggcgag aacagcaaga acagggcgaa aagaagcgtc
     2521 gacaggatgt gggccgatgc ggcgcccgtc acgatggcgc gcatgggcag gaggctgaag
     2581 ctcatgccga atctgatgag gaacagtccc gaaaacacgc ccgacaaaac gcccgccaag
     2641 ctcacgaacg cggtgcccgc gattccctcg aaccccgcaa gcggcataag cagcgtggac
     2701 gcgcacacgc cgaaagggag caggtaaagc gacagcttgg atttcaagaa acgtcccgta
     2761 gccctgtcgg ctataccggc aagcgcaagc gtcaacgtga cggcgatgat ggatatcagg
     2821 taggtggtat cgagcaggct ttcgccgtga tggtagcttg cgaacaggct catgctcttg
     2881 aagctcatcg acacccaggc gatgttgcag cccagcgctg ctcctacggt gatttctgca
     2941 gctgttggga agaccctttt tggattgacc ggctccatgg atttgtcccc atcccccccc
     3001 cgaatgcggg ctttccgccg tccgcaagcg aacggctccg agatgcctat tgtaccgcgc
     3061 tctcgtgccg gtcgagttcc gattgcgaca aaaagactgg gagatttgga aacgcgatca
     3121 gcgtttctcc aggtgaacgg ccatcgtgcg atgcgcacga taccgatttg atcgaaaacg
     3181 tgcgaccagg ccgatgtgcg acggcgacgg gatccatagg gtgaagcgca tcggagagga
     3241 cgatgaaagg aggaggaatg ggcgaactgc tcatgacccg acgtgcgttt gcgaaggtga
     3301 tggccgtgac ggctgcggcc gccggtttca cgggggctca gtcggcgttg gcggataccg
     3361 agccggctgc ctcgtcgggc gaagtcaagc gcatacgctc ggcctgtcgc ggatgcggca
     3421 agatggagtg cggcgtttgg gtgaccgttc aggatgggcg ggttgtcaaa acggagggcg
     3481 atgaaagcgc tttccagtcg gcgggaaacc attgcgcgaa agggcaggcg tccttgcagg
     3541 cggcgtatca tcccgaccgt ctcatgtacc cgctcaagcg cacgaatccc aaagggcaag
     3601 agcccggttg ggtgcgcatc agctgggatg aggcgtatcg atccaccgtc gaggcaatcc
     3661 ataagaacca ggaaaagtac ggcaacgaga cgtgcttctt catgggcggc acgtcgcgta
     3721 tctgggccat gggcccttat ggcgcgctga agcagtgctt cgggtcgccg aacggcatac
     3781 aggccaacga gatatgcaaa ggcccgcgct tctacgctac gaagctgaac gattcgaacg
     3841 cctacagctg gatggaagtg gtggggcgtc cgcgcgtgta cgtgcaatgg ggcggcgcgt
     3901 cggagctgtc caattacgac gatagctgtc gcaccacggt cgacgtggcg acgcgcgccg
     3961 acaagcacat cctggtcgat ccgcgccaga cgaacttggg gaaagaggcg gatatctggg
     4021 tgaaccttcg tcccggaacc gatggggcag tggcgaactg ctgggccaac gtgatcatcg
     4081 agaacgagct gtacgacgat ttgtacgtac ggaagtggat gaacgctccc atgttggtgg
     4141 tggaagatga gagcttcgag ccaacccctt cctcgtcgag cgcgcagacg gccaaggtgc
     4201 gtacgcgcct tctcaaagag tccgacctcg tcgagggcgg ggcggatacc cgcttcatgg
     4261 tgctcaacga gatcacaaac gagctgagct ggtacgatgc cggcggcgac aaccccggct
     4321 gggagggcga ggactgggtt cccgcaaccg agggcaaaga agctcatcag ccgggattgg
     4381 atctgacggg tcagacgcag ggcttcgtgc ttgactacgt accgtttccc gacgggctgc
     4441 ttcccgcgct gcataccccc gaaggcggct tcgaggtgga gttgaaagac ggctccagcg
     4501 tgcacgtgcg cacggtgtgg gagcgctata tcgagttctt ggaagactac actcccgaaa
     4561 aggtgtcgga gatatcgggc gttgacgtcg aagtgctaaa agaggcggcc atcacgtacg
     4621 caacgcgcgt cgacccttcg accgggtacg gcaatggcgg aatccagtac atgctggcgt
     4681 tggaacacgc gtgcaattcg acgcagaaca accgtgcctg cgacctgctg gcgggcataa
     4741 cgggcaacat ggacactccg ggcggcatgc gcggctcgac tcccggttgg cccgtctacg
     4801 acctcggcat gtgcgtgccc gactccggca aaaccaccga gtacaccatc gagaagattt
     4861 tgggcaaaga gcgttttccg atgatcggct cggactgcaa ccccagctgg gcggatgcga
     4921 cgtcggtgta cgacgctatc gagagcggcg agccttataa cgtgacgtgc ggcatcgggc
     4981 aaacgggcga cttcatgaac cagtcgaact ccctctttgc cgcggagcag cttcagaagc
     5041 tcgacttctg gtgctcgatc gatctgtggc acaccccgtg cgtggatatg atggccgata
     5101 tcgcaatgcc cgccgctcat tggctggagc tcgattgcat tcgcaaaagc cagggctcgt
     5161 cgggtgcgtt cggcgctacg gtgaaagcgg tggagcctcc cggagaagcg aagaacgatc
     5221 tggagatcgt ggttggcctg tataaggccg ccggggtccc gtacttcgac gaggagtatc
     5281 acggtgcggc gtggctcgag ggggatgaag ccgtggacgc gtgcaacaac gttgcgctca
     5341 aaagcttccg cattcccgat tggaatgact acaagaagga attccaagag aagggctggt
     5401 tcgattccaa ggtggagaag cccgacgatt ggggcgtcta ccggcgctat caaaccggaa
     5461 acggccacat caacggaggg ttcccgccca atccgaacca gcatcaaggc tggaacacga
     5521 ccacgcacaa gcaggagatc tggtcgacgg tgctggaatc atggttgccc ggcgagggcg
     5581 aggagttccc gaaattcgtg gaggctccgc atggccccgt cgccgacccc gatttgttca
     5641 cggacgacaa ctcgttcttg atgaccaccg ggcgtcgtca aggaacctac ttccattccg
     5701 agcaccggca gctgccatgg tgccgcgagc tgtggcccgt tcctcggctt gagatgaatc
     5761 ccgttgacgc cgagcgactt ggtctcgagc agggggattg ggtatggatc gaaaccgatc
     5821 agcacaagat tcgcgaagtg gtcgatctgt actacggcat cgccccgggt gtggtgaacg
     5881 ccgagcacca atggtggtac cccgagctca atcagcccga tcacgggttc aaattgtccg
     5941 gcgtgaactg tttgatcgat cgccatgccc aagatcgcat catcgggtcg tcgaacctgc
     6001 gtgcttacgg tgtgaaggtg tacaaggcca cgcccgagaa ttcgccgttc ggcaaccccg
     6061 tgccgtgcgg agacgacggg acgcccatca tccacacctg cgacgatccg cgcctgaagg
     6121 aatggcaacc gttgtacgag gggagggagt gacctatggc tcgttatgcg attctgaccg
     6181 atctcaacaa atgcgtcggc tgccttgcgt gctcgatagc gtgcaaggtg gtgaacagcg
     6241 tgccggtggg gtcgtattgg aacaaggtct tgcgcatcgg accgaacccg cgcaccaagg
     6301 gcggccaatg gcccgacgtg tacacctatt tcctcaccgt gcagtgccaa cactgcgaga
     6361 atcccgagtg cgtgaaggtg tgcccgaccg gcgcctctca caagctggag gacggcaccg
     6421 tgcagatcga caagtcgaag tgcatcggct gccagttctg cgcgatgtcg tgcccgtaca
     6481 gcgttcgcta cttgaacgag gaggagggcg tcgtggagaa gtgcacgctg tgcgagcaga
     6541 agatcgccca gggcgagctg ccgcaatgcg tgagcgaatg cggcggtcgc gctcggttct
     6601 tcggcgattt ggaccagggg ctcgagagct ttgaggcgcc ggggtgcgat ctgcaggacc
     6661 cgtcgtacga tgcgcaggcg aaggccagga tgaagtgggg cgatttgata ggcgactcat
     6721 gcgactatcc ggtgaagcct tacgacgaga acgaagtgta ccaccttccc gacgtgggca
     6781 acgcccccag cttcgtctac atcctgcgaa acgagaagtg gcaaggaggc ggtgaatgat
     6841 ggaactccaa tggcctttga ttttgttcac cacgctggtg gcgtggagcg cggggctgtt
     6901 cgggacgcag gctctgatgg cggtgttcgg ggtggggaag cgggcgcagg tcccggcttg
     6961 ggttgcgtcg gcggtgctgc tcgcggccgg cgggatcgcg gtgttcttcc acctggagca
     7021 ctgggagcgg atcttcaacg ggttcgggca cctgacgagc gggatcacgc aggaattgat
     7081 cgcgatcgtg gtgctggcgg tcgtcgcggt cgcgtatctc gtgctcatgc gcaagtccga
     7141 cgacggcgcg agcgtgccga agtggctggc ctgggtcgcc gtcgccttgt cggtggtgct
     7201 cgtggccgtc atggcgcact cgtacacgat ggccgcgagg ccggcgtggg actcggtgct
     7261 ctggatcctc tacgtcctcg gcaacgcctg cgtcctgggc cccgcgactt tcctcctggt
     7321 cttggctgca gggggacccg gggaccagcc cgccgatcgc gcagccgacg ccggggcccc
     7381 cgcaggtagg acggccctcg ccggcgccgc cctcaacgcc ctcgcggccc tcgccttcgc
     7441 gatcttcctc cagctctccg ccggctcctt cgccgacgtc ggcctctact tcgacccgac
     7501 gcaccccacc aaggccatgg ccgacgcagc cgcgacggtc gcctcccagg ccccgctcct
     7561 gtggctgggc gccgtggccg tcggcgcgat cgtccccctg gccgccgcct tcctaggcag
     7621 gcgcacgggg aactggaagc tctgggcccc ggcagccata atcgccgccc tcgtcggagc
     7681 cgtctgcatg cgcgtcgtct tctacaacct ggggctctcg gtcttcatgt tctactagac
     7741 tagtcgactc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
     7801 gaatataaaa agccagatta ttaatccggc ttttttatta tttaattcct gttataaaaa
     7861 aaggatcaat tttgaactct ctcccaaagt tgatccctta acgatccctg caggagaagg
     7921 ccatcctgac ggatggcctt tttgaagctt tctagaatcg ggggcccccg gggggattct
     7981 agaacaatgg aagtcctcgt ctcgccctcg aattagcccg cttaatgagc gggctttttt
     8041 tgaattaatt ctcgcgagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8101 caacgcaatt aatgtaagtt agcgcgaatt gcaagctggc cgacgcgctg ggctacgtct
     8161 tgctggcgtt cgggagcaga agagcataca tctggaagca aagccaggaa agcggcctat
     8221 ggagctgtgc ggcagcgctc agtaggcaat ttttcaaaat attgttaagc cttttctgag
     8281 catggtattt ttcatggtat taccaattag caggaaaata agccattgaa tataaaagat
     8341 aaaaatgtct tgtttacaat agagtggggg gggtcagcct gccgccttgg gccgggtgat
     8401 gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca gctccggcaa
     8461 cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct ctgacggcca
     8521 gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt cgatccagcc
     8581 acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca cctcgtccat
     8641 gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg ggttcagggc
     8701 cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc gaaacccctg
     8761 ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga tgcagtcctg
     8821 tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg ccagcgcccg
     8881 atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg gttccaggaa
     8941 cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta ggccattagg
     9001 cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca gcggctccgg
     9061 gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca gcttgctgcg
     9121 cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt gcgccgggtc
     9181 gtgtcggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc acccccttgc
     9241 tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc cgcctgaacc
     9301 accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg acgaagaacc
     9361 ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg ctcgacaggt
     9421 aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc tgctgctggt
     9481 cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg atagagcacc
     9541 cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg ccgctggcgt
     9601 tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg cggccctcgg
     9661 cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg ctgccgatca
     9721 gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg aggtgcgccc
     9781 caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc aggtagatca
     9841 ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca atctgtgcgg
     9901 ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc gccccgaccg
     9961 taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct gcgaacgcct
    10021 ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg cagttggtgg
    10081 ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt ccaggcactc
    10141 gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca tccctttggc
    10201 cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca gcaggtcgcc
    10261 ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg gggccgaaag
    10321 gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg gccatatcag
    10381 cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca ccgggcaacc
    10441 aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt tcgtattcca
    10501 taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac caacgcaagc
    10561 actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt gccggtgccc
    10621 gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg ctgacggtgc
    10681 gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg ctggcctcgg
    10741 ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc tgcgcggcga
    10801 taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc atctcgctgc
    10861 ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcggac agttcgaggc
    10921 tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc tggccagcct
    10981 gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc agcagcaccc
    11041 acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag cccgccaagc
    11101 ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg gccagcgtcc
    11161 gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc catgcctgat
    11221 agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct ttcatgtcat
    11281 ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc tgctcggcgg
    11341 gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc gtgttctgga
    11401 gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg tccctgacgc
    11461 cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc tgcaaagtcc
    11521 tgtcgttctt catcgggcca ccaagcgcag ccagatcgag ccgtcctcgg ttgtcagtgg
    11581 cgtcaggtcg agcaagagca acgatgcgat cagcagcacc accgtaggca tcatggaagc
    11641 cagcatcacg gttagccata gcttccagtg ccacccccgc gacgcgctcc gggcgctctg
    11701 cgcggcgctg ctcacctcgg cggctacctc ccgcaactct ttggccagct ccacccatgc
    11761 cgcccctgtc tggcgctggg ctttcagcca ctccgccgcc tgcgcctcgc tggcctgctg
    11821 ggtctggctc atgacctgcc gggcttcgtc ggccagtgtc gccatgctct gggccagcgg
    11881 ttcgatctgc tccgctaact cgttgatgcc tctggatttc ttcactctgt cgattgcgtt
    11941 catggtctat tgcctcccgg tattcctgta agtcgatgat ctgggcgttg gcggtgtcga
    12001 tgttcagggc cacgtctgcc cggtcggtgc ggatgccccg gccttccatc tccaccacgt
    12061 tcggccccag gtgaacaccg ggcaggcgct cgatgccctg cgcctcaagt gttctgtggt
    12121 caatgcgggc gtcgtggcct gcccgctcta atgcccggtt ggcatggtcg gcccatgcct
    12181 cgcgggtctg ctcaagccat gccttgggct tgagcgcttc ggtcttctgt gccccgccct
    12241 tctccggggt cttgccgttg taccgcttga accactgagc ggcgggccgc tcgatgccgt
    12301 cattgatccg ctcggagatc atcaggtggc agtgcgggtt ctcgccgcca ccggcatgga
    12361 tggccagcgt atacggcagg cgctcggcac cggtcaggtg ctgggcgaac tcggacgcca
    12421 gcgccttctg ctggtcgagg gtcagctcga ccggcagggc aaattcgacc tccttgaaca
    12481 gccgcccatt ggcgcgttca tacaggtcgg cagcatccca gtagtcggcg ggccgctcga
    12541 cgaactccgg catgtgcccg gattcggcgt gcaagacttc atccatgtcg cgggcatact
    12601 tgccttcgcg ctggatgaag tcggccttgg ccctggccga ttggccgccc gacctgctgc
    12661 cggttttcgc cgtaaggtga taaatcgcca tgctgcctcg ctgttgcttt tgcttttcgg
    12721 ctccatgcaa tggccctcgg agagcgcacc gcccgaaggg tggccgttag gccagtttct
    12781 cgaagagaaa ccggtaagtg cgccctcccc tacaaagtag ggtcgggatt gccgccgctg
    12841 tgcctccatg atagcctacg agacagcaca ttaacaatgg ggtgtcaaga tggttaaggg
    12901 gagcaacaag gcggcggatc ggctggccaa gctcgaagaa caacgagcgc gaatcaatgc
    12961 cgaaattcag cgggtgcggg caagggaaca gcagcaagag cgcaagaacg aaacaaggcg
    13021 caaggtgctg gtgggggcca tgattttggc caaggtgaac agcagcgagt ggccggagga
    13081 tcggctcatg gcggcaatgg atgcgtacct tgaacgcgac cacgaccgcg ccttgttcgg
    13141 tctgccgcca cgccagaagg atgagccggg ctgaatgatc gaccgagaca ggccctgcgg
    13201 ggctgcacac gcgcccccac ccttcgggta gggggaaagg ccgctaaagc ggctaaaagc
    13261 gctccagcgt atttctgcgg ggtttggtgt ggggtttagc gggctttgcc cgcctttccc
    13321 cctgccgcgc agcggtgggg cggtgtgtag cctagcgcag cgaatagacc agctatccgg
    13381 cctctggccg ggcatattgg gcaagggcag cagcgcccca caagggcgct gataaccgcg
    13441 cctagtggat tattcttaga taatcatgga tggatttttc caacaccccg ccagcccccg
    13501 cccctgctgg gtttgcaggt ttgggggcgt gacagttatt gcaggggttc gtgacagtta
    13561 ttgcaggggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg gagtgacggg
    13621 cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg
    13681 ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg
    13741 cggccctcgc gagaattaat tcagataaaa aaaatcctta gctttcgcta aggatgattt
    13801 ctagcgatga ccctgctgat tggttcgctg accgtagact ggttggcttg gtttcatcag
    13861 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
    13921 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
    13981 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
    14041 cacgaaccct ttggcaaaat cctgtatatc gtgcgacatc gcgcgcgcgc gcgcggatgc
    14101 tagcggcgcg ccaaaaaaaa gcccgctcat ta
//
