LOCUS       sequence-464774-       14575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta DSM 2243 El Vadh without accessory genes
            (inactive) in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14575)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 14575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN6-Elen0497-ElVadh-noAccs(ElDSM2243)
FEATURES             Location/Qualifiers
     source          1..14575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      8199..8226
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      8358..8385
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(8817..9668)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(9655..10494)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(11005..11976)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            13215..13302
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      13644..14038
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(14201..14244)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            14340..14449
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttac
     1501 gcgacttctt cctctgagcc ctccaccagg tccatcagct cttgctgggt gtggatgtcg
     1561 agctttttgt agatgtggcg catatgggtg cgggcggtgc cctcggagat gtagagctgc
     1621 tcctggatgt aggcggcgtt gcggcctttc gccagcagga acaggacttc tgtttccttc
     1681 ttcgacagga ggtagcggtt agccaccagc tcgcacttgc gcttgaaccg cccggcgcgc
     1741 gctttttcgg gggactcggc gcgctcgcgc ccgcggagca tctcgcctgc cgggttgtcc
     1801 gattcaccca cgatgagggc gcgcagctcg ctgccgcgcg gcagggcgcc gaagccgaaa
     1861 atgatgcatg cgaacacgat gatcagcgtg ttcgccggat tggttgcgga gtcgagcgcg
     1921 gggatggcga aggaggccgt catcccgatg agcgcgccca gcgcgagtgc cccgcgtccg
     1981 aacccgaaca ccacgaacga cgtcaggcgg aaacgctggg tgatgtcggc gtagctcacc
     2041 cacatggtgc cctcgaacag gaggtagcac agcagcagcg cgaacgtggt gaagggcgtc
     2101 gcgtcggcga taaagtagac cgcgaccagc gcgatcgcga taaggggcag cagggggcgg
     2161 aacatgaagt tgatgtgctg gcgctgcgtt agcatgacgg cgatcatgag cgcgcacgcc
     2221 gtgagcgcgg ccgccccgat gcagacgacc tgcgttccca ggccccctac caagtgcatg
     2281 tcggcgatgg cttgcgtgcg cacggcgccc agcgcgaacc cgaacagcag gctgggcgcg
     2341 cacaggcgca gcgcgaacgg ggttttcctc accttgagcg tgatggcggc gaactcgagc
     2401 ttgtcgacga gcgcggtgct gcaccggtag aggcaccagc actcgaggaa cgggatgagg
     2461 gccgcggcca gggctcccag cggatggagc aggtccaggt tagacactgc cgtgtacagc
     2521 gcgatgcccg ccaccagcga gagcgccgta ctggtgacga tggtggcgat gtcgcattgc
     2581 ccgaacgaca cgccgtagct catcaggagg atggccgagc cgatgccggt ggtcgcacct
     2641 cccgccgcga gcgcggcgat gcccgctgcg cttccgacgt ccgccaagca catgaacagc
     2701 gtgccggcga aaccgagcgt cgcgccgatg aaccgtaggc gtgcgcgtcg gcgctgcgtg
     2761 gcgaacaaac ggcggtatgc gcgcgatagc acgccgtagg acagcagcgt catgacgaag
     2821 aacacgagcg acacggggtg gaagagcccg gccggcaccg ccggatcggc cgcgttccag
     2881 aagaacagct gctggccgtt gaacatcgtg aggtagaccc acgcccagtg ggcgccgaag
     2941 ccgacgatga gcgctggcga cagctttccc agcgagaacc cctcatcctc gcgcacctcc
     3001 actttgaccc gcggaagctt gatttccatc aggtacccgc tttcccctcg tctgcgaacc
     3061 cttgctgttc atcatactgc accgtcatcg ggttgctgct ccgcgcctgc cacctttccc
     3121 ctttgtcgat ggctgtttcc tggggttttg cgaaaaatca tagccacggt atgatgcccc
     3181 gccactccgt ctcgacgcaa aatcatagcg ctgctgggag gggatgcccg ccttcgccgc
     3241 ttaggatggc tccatcgttt caggtatgaa gcgagagacg agaaaggaag gtggggatca
     3301 atgactcagc tcacgctcac gcgccgcagc ttcatgaagg cgatggcggt caccggtgcc
     3361 gctgcgtcgc tcacggcggt cgccgagccg atgcgggcgc ttgccgaggg cgtcgatgcc
     3421 gatgcggggg aagtcaagcg cgttcgctcg tgctgccgcg cttgcggcaa ggtggagtgc
     3481 ggcgtttggg taaccgtgcg cgacggcaag gtcgtcaagg tggaaggcga cgagtccgcg
     3541 ccgcaaagcc gcgggcactg ctgctcgaag tcgcagtcat cgatgcaggc gctatatcat
     3601 cccgaccgcc ttcgtttccc ggtgaagcgc acgaacccta agggcgagga cgatcccggt
     3661 tgggtgcgca tcacgctgga cgaggcgttc gagatatgcg gcgagaagct caaggaagtg
     3721 aaggagaagt acggcgggga gtccatcttc gtgatgtgcg gcacgtcgcg cgtgtggtcg
     3781 ctcgggcctt accagggcat gaagcagctg ttcggcacgc cgaacgcgca tttggcctac
     3841 caggtgtgca aaggcccgcg ccacttcggc ggcatcatga ccgacgagat ggggtccccg
     3901 tggatggagg tggaggccga gcccagcgtg tacgtgcagt ggggcacggc gtgcgagtac
     3961 tccaactacg actccacgaa ccgcacggtc accgacgtcg cgcatcgtgc gtctaagcat
     4021 atcgtggtgg atccgcgcgt cacgcctttg ggcaaggaag ccgacatctg gctgccgctt
     4081 cgtcccggca ccgacggcgc gctggcgctg tcgtggctca actggatcat cgaaaacgaa
     4141 gcttacgatg acacgatggt gcgtcgctgg tcgaacgcgt cgttcctcta cgtggacgac
     4201 aagcccgagc tgacgcaagg ctggttggtg gagggcaacg gcggcatcaa catgaagacg
     4261 aagctgctga ccgaggccga cctcaaggaa gatggcaagt accagcgctt catggtgtgg
     4321 gacgaggcga acgagcgtct cacgtactgg gatgccgagc ttggcatgtg ggagggcgag
     4381 gagcaccgca tccccaccac cggcacatgg atcgagcatc cgtacaagcc gcttgttgcc
     4441 gatgcatggc tgcccgatca gtcgacgttc gccgatccgg ctaccgagcc cgaccgcttc
     4501 cccgacggtt tcgaggaatg caatcccaag gggttgccga agcgcccatc tctgcttcct
     4561 ggtgaggtag aggtaacgct caaagacgga accgtgcaca aggctcgctc tgtgtgggat
     4621 gcgttccatc agatgaccag cgagtacacc atggagaagg cagaggagat caccggcgtg
     4681 ccggcggcga agagcgagga ggctgtacgc gcctggacga cgcgcgtcaa tccgctgcat
     4741 ggcaacggcg gcatccactt ccagctggcc accgaccaga acggcaactc catccagaac
     4801 gtgcgtgcgc tgcagatcct gtcgtgcatc accggcaact ccgacgagcc ggccggcaac
     4861 cgcggctcgt cgaaggcgca gttcgacggc aatccagggc gttcgaacat gcaggccggc
     4921 gcgccgtacg gcgacgaggc caagatgtgg gacggccgcg acgtcgacct cgaagggctc
     4981 gccgcgaaga tgcaggattt cgtgcagtac ctcatcgaca acgattcgcc cctggccgag
     5041 cgctacggca acaaggtgcc cagccatgag gaagccctcg ttatcgccaa gcgcatgggc
     5101 ggcgcattcc gcacgtcgca ggcgtggccg aaccccaaga ccgtgttcga gcgacaagcc
     5161 aacgaggttg acgccgagcg attccccctc aaccgctact gggcgcgctg ggcggacgcg
     5221 aactccatct gggacggctg cctggacaac gacgtgccgt accagttgca tgcgggcgtg
     5281 tgccagtcgg gcgacttcat gaatatgggc aacatcgacg tggcgtggga ggccatgacg
     5341 aagctggact tcttcaccga catcaacctg tggttctgcc cgaacaacgg caacgccgac
     5401 gtcatctttc cgtgctacca ctggttggaa gttgacacga cgcgcgtcag ccagggcgcc
     5461 ggcgggttct tcggctgcgg gtgcgccgct gtcgaggcgc cgggcgagtg catctacgat
     5521 cccgactgga acgtgggcat gtacaaggcc atgggcgtgc catggaacac gaaggacgag
     5581 tcggccgagc cgtccgagat cctcaacttc ggcgagaaga ccgactaccg ttggccgaac
     5641 cgcagccgcg tgctcaagga caacgtggac gcgtggaaga cagccgagtt ccccgacggt
     5701 cccacgtggg agcaagcgaa ggagcacttc cagaagaacg gctggatgga ttgccgcacg
     5761 tggcatcccg agcgttgggg cacgtatcgc cggcacgaga tgggctggcg tcgccagcag
     5821 ggcggcttca acctgttccc gttggtggac gaccatccgg gcttcatgac gccgtcgggc
     5881 ctcattgaaa tctggtcgct tgtgtgcgag gcgtacttag gcgacgagga caagttcccc
     5941 gtgtaccgcg aacccacgaa ctcgccggtg accacgcccg agtacttcga cgccgcgaag
     6001 gttgccgaca tcgatgagtc gaagctgcgc aatatggatt atcccgccag cctgcaggag
     6061 cacgccgatg cgacgttcct catgaccacg ggcgcgcgcc agccggtgta cttccacagc
     6121 gagcatcgcc agctgccgtg gtgccgcgag ctgtggcccg cgccgcgcct ggagatgaac
     6181 ccgaacgacg ctgcgcgtct ggggctcgag cagggcgact gggtgtggat cgagagcccg
     6241 tggggcaagg tgcgcgaggt gctggatctg tactacggca tcagcaaggg cgtggtgaac
     6301 gcgaaccacg cgtggtggtt ccccgagatg gacacggcca gccacggcta cgagctggtg
     6361 aacatcaact gcgtgatgga tccttacggc caggatgtgg tgtgcggtgc agcaacgatg
     6421 cgctcggttc ccgtgctggt ttataaggct acggcggaaa actcgccgtt cggcaacccg
     6481 gtgccgtgcg atccgcaggg taacccgtgc atctgcgatg cgggcgatcc gcggttgaag
     6541 gaatggatgg gaacgggatt gcgatctcgc gttgaaggcg aagaggcgtg gaccggagag
     6601 ggggcgtaga cgatgacaca gtacgcaatc atcaccgatt tgaaccggtg cgtcggatgc
     6661 cttgcgtgca cggtcgcatg caaagcggtg aacaacgtgc ccgtcggggc gttttggatc
     6721 aagacgctgc gcgtgggtcc gaatccgatc gagggcggaa gcggcgattg gcccgacgtt
     6781 gagatgtatt tcctgcccat ccaatgccag cattgcgccg atcccgagtg cgtgaaggtg
     6841 tgtcccacgg gcgcctcgca taaggcagcc gacggcaccg tgcagatcga caagtcgaag
     6901 tgcattggct gccagttctg cgcgatgagc tgcccctaca acgttcgcta cctcaacgaa
     6961 gaggagcgcg tggtggagaa gtgcacgctg tgcgagcaga agatcgccca aggcgagctt
     7021 ccgcagtgcg tggcgcaatg cggctcgcgt gcgcgcttct tcggcgactt ggagcagggc
     7081 gtggacgggt tcgaggcacc ggctccgcct caagccctgt cgtgcgacta cgccgaaatg
     7141 cagcagacgc gcgtcacgct gaaggagtac gtcgaaccgt atacggaaga cgagatccat
     7201 cgtctgcccg atgttggaaa tggcccggag ctcatgtacc tgctgcgctc cgaccgaaag
     7261 tggagggggt agaccatgga actgcaatgg cctttgatcg tgtttaccac cctggttgcc
     7321 tggtcggcgg ggctgttcgg gacgcaggcg ctgatggcgg tgttcggcgt cgggaagaag
     7381 gcgcaggtgc cggcttgggt ctgctcggcc gtgctgctgg cggccggcgg gatcgcggtg
     7441 ttcttccatc tcgagcactg ggagcggata ttcaacgggt tcgggcacct gacgagcggg
     7501 atcacccagg aattgatcgc gatcatcgtg cttgccgtgg tggctatcgt ctacttggtg
     7561 ctcatgcgca agtccgacga cggcgcgagc gtgccgaagt ggctggcctg ggtcgccgtc
     7621 gcgctgtcgg tggtgctggt cgccgtgatg gcgcactcgt acacgatggc cgcccgcccc
     7681 gcctgggact cggccctctg gatcctctac gtcctcggca acgcctgcgt gttgggcccc
     7741 gcgacgttcg ccctcctctc cgccttggct gcagggggac cccgggacca gcccgccgag
     7801 cgcgcagccg acgccggggc ccccgcgggt aggacgcccc tggtaggcgc agccgccaac
     7861 gccctcgcaa ccctcgcctt cgccgccttc ctccagctct ccgccggctc cttcgccgac
     7921 gtcggcctct acttcgaccc gacgcacccc accaaggcca tggccgacgc agccgcgacg
     7981 gtcgcctccc aggccccgct cctgtggctg ggcgccgtgg ccgtcggcgc gatcgtcccc
     8041 ctggccgccg ccttcctagg caggcgcacg gggaactgga agctctgggc cccggcagcc
     8101 ataatcgccg ccctcgtcgg agccgtctgc atgcgcgtcg tcttctacaa cctggggctc
     8161 tcggtcttca tgttctacta gactagtcga ctcacactgg ctcaccttcg ggtgggcctt
     8221 tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta
     8281 ttatttaatt cctgttataa aaaaaggatc aattttgaac tctctcccaa agttgatccc
     8341 ttaacgatcc ctgcaggaga aggccatcct gacggatggc ctttttgaag ctttctagaa
     8401 tcgggggccc ccggggggat tctagaacaa tggaagtcct cgtctcgccc tcgaattagc
     8461 ccgcttaatg agcgggcttt ttttgaatta attctcgcga gctggcacga caggtttccc
     8521 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagcgcga attgcaagct
     8581 ggccgacgcg ctgggctacg tcttgctggc gttcgggagc agaagagcat acatctggaa
     8641 gcaaagccag gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa
     8701 aatattgtta agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa
     8761 ataagccatt gaatataaaa gataaaaatg tcttgtttac aatagagtgg ggggggtcag
     8821 cctgccgcct tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag
     8881 cccagcgcga ccagctccgg caacgcctcg cgcacccgct ggcggcgctt gcgcatggtc
     8941 gaaccactgg cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg
     9001 gttttgccgg ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg
     9061 tccagcgccc gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc
     9121 tgcgcgatca aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac
     9181 tccgacagca gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc
     9241 caaaggcgct cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc
     9301 ccgatttcct ttgccagcgc ccgatagcta cctttgacca ccatggcatc agcggtgacg
     9361 gcctcccact tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc
     9421 gggccaagca ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga
     9481 tcatcagcgc ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca
     9541 tacgtcacgt ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg
     9601 ggggccagac agtgcgccgg gtcgtgtcgg acgtggctga ggctgtgctt gttcttaggc
     9661 ttcaccacgg ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac
     9721 cccgccgtca tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct
     9781 cacaccgaag cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc
     9841 ggcgctggtc atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct
     9901 gccccggctg gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg
     9961 gtgcaggaac acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac
    10021 ctgggccatg gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag
    10081 caccatcagg cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat
    10141 gatgttgggc aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg
    10201 ttcctcggcg ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg
    10261 gtcttcggcg ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc
    10321 cggcccgcct gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc
    10381 gggcgacacc agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg
    10441 tggcggcgct gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg
    10501 cctcctttgc aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc
    10561 gctctgagtt cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac
    10621 ttgcgctgac gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg
    10681 tcagggctgg ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc
    10741 gagaaacttg ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc
    10801 aaggttaagg ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt
    10861 ataaccaaag ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc
    10921 tgaagcgctt ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa
    10981 caggcgtgag taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc
    11041 tcgctggcgg ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag
    11101 acccatgacc ttgctgacgg tgcgctcgat gtaatccgct tcgtggccgg gcttgcgctc
    11161 tgccagcgct gggctggcct cggccatggc cttgccgatt tcctcggcac tgcggccccg
    11221 gctggccagc ttctgcgcgg cgataaagtc gcacttgctg aggtcatcac cgaagcgctt
    11281 gaccagcccg gccatctcgc tgcggtactc gtccagcgcc gtgcgccggt ggcggctaag
    11341 ctgccgctcg gacagttcga ggctggccag cctgcgggcc ttctcctgct gccgctgggc
    11401 ctgctcgatc tgctggccag cctgctgcac cagcgccggg ccagcggtgg cggtcttgcc
    11461 cttggattca cgcagcagca cccacggctg ataaccggcg cgggtggtgt gcttgtcctt
    11521 gcggttggtg aagcccgcca agcggccata gtggcggctg tcggcgctgg ccgggtcggc
    11581 gtcgtactcg ctggccagcg tccgggcaat ctgcccccga agttcaccgc ctgcggcgtc
    11641 ggccaccttg acccatgcct gatagttctt cgggctggtt tccactacca gggcaggctc
    11701 ccggccctcg gctttcatgt catccaggtc aaactcgctg aggtcgtcca ccagcaccag
    11761 accatgccgc tcctgctcgg cgggcctgat atacacgtca ttgccctggg cattcatccg
    11821 cttgagccat ggcgtgttct ggagcacttc ggcggctgac cattcccggt tcatcatctg
    11881 gccggtggtg gcgtccctga cgccgatatc gaagcgctca cagcccatgg ccttgagctg
    11941 tcggcctatg gcctgcaaag tcctgtcgtt cttcatcggg ccaccaagcg cagccagatc
    12001 gagccgtcct cggttgtcag tggcgtcagg tcgagcaaga gcaacgatgc gatcagcagc
    12061 accaccgtag gcatcatgga agccagcatc acggttagcc atagcttcca gtgccacccc
    12121 cgcgacgcgc tccgggcgct ctgcgcggcg ctgctcacct cggcggctac ctcccgcaac
    12181 tctttggcca gctccaccca tgccgcccct gtctggcgct gggctttcag ccactccgcc
    12241 gcctgcgcct cgctggcctg ctgggtctgg ctcatgacct gccgggcttc gtcggccagt
    12301 gtcgccatgc tctgggccag cggttcgatc tgctccgcta actcgttgat gcctctggat
    12361 ttcttcactc tgtcgattgc gttcatggtc tattgcctcc cggtattcct gtaagtcgat
    12421 gatctgggcg ttggcggtgt cgatgttcag ggccacgtct gcccggtcgg tgcggatgcc
    12481 ccggccttcc atctccacca cgttcggccc caggtgaaca ccgggcaggc gctcgatgcc
    12541 ctgcgcctca agtgttctgt ggtcaatgcg ggcgtcgtgg cctgcccgct ctaatgcccg
    12601 gttggcatgg tcggcccatg cctcgcgggt ctgctcaagc catgccttgg gcttgagcgc
    12661 ttcggtcttc tgtgccccgc ccttctccgg ggtcttgccg ttgtaccgct tgaaccactg
    12721 agcggcgggc cgctcgatgc cgtcattgat ccgctcggag atcatcaggt ggcagtgcgg
    12781 gttctcgccg ccaccggcat ggatggccag cgtatacggc aggcgctcgg caccggtcag
    12841 gtgctgggcg aactcggacg ccagcgcctt ctgctggtcg agggtcagct cgaccggcag
    12901 ggcaaattcg acctccttga acagccgccc attggcgcgt tcatacaggt cggcagcatc
    12961 ccagtagtcg gcgggccgct cgacgaactc cggcatgtgc ccggattcgg cgtgcaagac
    13021 ttcatccatg tcgcgggcat acttgccttc gcgctggatg aagtcggcct tggccctggc
    13081 cgattggccg cccgacctgc tgccggtttt cgccgtaagg tgataaatcg ccatgctgcc
    13141 tcgctgttgc ttttgctttt cggctccatg caatggccct cggagagcgc accgcccgaa
    13201 gggtggccgt taggccagtt tctcgaagag aaaccggtaa gtgcgccctc ccctacaaag
    13261 tagggtcggg attgccgccg ctgtgcctcc atgatagcct acgagacagc acattaacaa
    13321 tggggtgtca agatggttaa ggggagcaac aaggcggcgg atcggctggc caagctcgaa
    13381 gaacaacgag cgcgaatcaa tgccgaaatt cagcgggtgc gggcaaggga acagcagcaa
    13441 gagcgcaaga acgaaacaag gcgcaaggtg ctggtggggg ccatgatttt ggccaaggtg
    13501 aacagcagcg agtggccgga ggatcggctc atggcggcaa tggatgcgta ccttgaacgc
    13561 gaccacgacc gcgccttgtt cggtctgccg ccacgccaga aggatgagcc gggctgaatg
    13621 atcgaccgag acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa
    13681 aggccgctaa agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt
    13741 agcgggcttt gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg
    13801 cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg cagcagcgcc
    13861 ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat ggatggattt
    13921 ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg cgtgacagtt
    13981 attgcagggg ttcgtgacag ttattgcagg ggggcgtgac agttattgca ggggttcgtg
    14041 acagttagta cgggagtgac gggcactggc tggcaatgtc tagcaacggc aggcatttcg
    14101 gctgagggta aaagaacttt ccgctaagcg atagactgta tgtaaacaca gtattgcaag
    14161 gacgcggaac atgcctcatg tggcggccct cgcgagaatt aattcagata aaaaaaatcc
    14221 ttagctttcg ctaaggatga tttctagcga tgaccctgct gattggttcg ctgaccgtag
    14281 actggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
    14341 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
    14401 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
    14461 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgac
    14521 atcgcgcgcg cgcgcgcgga tgctagcggc gcgccaaaaa aaagcccgct catta
//
