LOCUS       sequence-464775-       11421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80EN13-Elen0616-Cber(ElDSM2243)
FEATURES             Location/Qualifiers
     source          1..11421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      5045..5072
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5204..5231
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5663..6514)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6501..7340)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7851..8822)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10061..10148
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10490..10884
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(11047..11090)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            11186..11295
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcaa
     1501 cccttcgtca ggcgcaggat gtcctcgcgc gaggagacgt cgagcttgcg atagatgttg
     1561 tggatgtggg tgcgcaccgt gttcggcgag atgaacagca cgtcggagat gtaggcgccg
     1621 ctgtggcctt cggcgaggta ggcgaggatt tcctgctcgc gcgccgtgag gttgtgcgcg
     1681 tccgccagct gggcgcagcg gtcgggcgag cttgcgggcg tcgcctcgtc ctcggcgtgc
     1741 tcggcggcgc gctgttcgtc gtgcgcatcg cgggtgcggc gcatgtacga cgagagcacc
     1801 atgacgaacg cgtacagcgt ggtggtgacc gtcaccgcca catggacgag gtcgtcgtcg
     1861 agcacgctcg cgaagctctg gccggcgaac gaggctgcgc agaacagcag cacggcggtg
     1921 gagaacacga ggtcggccga gaactcgccc gcgttcgcga tggcgcatag cgtggccagc
     1981 gtgaggatgg ccgccagcgc gtacagcgcg taggtcagaa acgacacgag tcccgacccg
     2041 ctgcccaccg atgccgagat gctggtggcc gccagcagca ccatggccat gagcggcagg
     2101 aacgtctggt gcatgccgcc tcgcagcgcg aagcgctttt tgcgaagggt cgtgtacgcc
     2161 agcagcgtcg cggcatcgag cgcgagcgcg gccaggtagg cgttctggct ctcgttgaac
     2221 gcggtgcgca tgacggccat cgcgaacgcg aacacgagca gccccagccc gggcgcgacg
     2281 accacgtcgg ccagcgaggc cagggtggcg cccgtcgtgc ggccctcggc gcgttcgggg
     2341 gcggtctgct cggtgctgtc cgccgttccc gagaacgcga gagggacgat gacggccgcg
     2401 atggagctga gcacgaacag caccgtcacg cccggcaggg gcagcacggc gtacagaaag
     2461 cagatcccgg ccgacagcat gctggcaagg gaaacggcga cgagcgcgcg cttgattttg
     2521 aaacgcgcgc agatgcgccc ccacgtgagc gccaagcaca cgtcgccgag ggcggcggcc
     2581 acggcaagcg cgatgagcag cgccgtaccc aagctgccga accaggagaa gtacgcgaac
     2641 gcgagcgcgc agcacaggta cagcatgccg cccccggcgc ccagcagcat ggacggcagg
     2701 cgctttcccg ccagcgacaa gccgaggaac agcgcgccga acagcgtggc cgccaacagg
     2761 gtggtttcgg cgaaggtgcc cacgaacagc aacgtatgct cgaagcgcag aaacgagatg
     2821 accgttcccg aatagatgag cagcgtggag aacaggcagg cgacgccaac gatgagcgct
     2881 gtcatcgcag gcaaggagag cccttcgccc gctcgcccgc gttcgagctt gcccgttgtt
     2941 tcgcgttgcg tctcgatagc cgcctccttc cctcgccgtg cgtcgcatta tagcattcgc
     3001 gcgtctcacc tgcgtaaaca atcgacgttg aggcgtctac gtaaaatcgt tgagaagcat
     3061 tcgccatctg cggaaacgca aatcaacgtt ttttcggatg gaagcgtgcg tcggttcggg
     3121 caaagatggg gtcgcagggt acgaagcaca acgcaaagga gatggggaac atggaacttt
     3181 ctcgcaggaa cttcttggtg ggcgccgcca gcgccggcgt cctcggcgcg atggccgggc
     3241 tgaccggatg cgcgccgcag gcgtcgtccg cgtcggctaa gaacggcgac gcgtcgtccg
     3301 ccgacgcggc ggtcgcgcgt ccgacctatt acatgtgcga cgaggattgg ctgggaagcg
     3361 cgcccgagat cgccgacgcc gacatcaaag agacgaagag cttcgacgtc gtggtggtgg
     3421 gcggcggcca tgccggcacg caggcggcgc ttgcggcggc gcaggagggc gcgaaggtcg
     3481 ccgtcatcga gaagcacaac gacggcgaga tcgtttaccg cggcgacgac atctgctcgt
     3541 acaactccaa gatgctggag gcgtggggct tcggcccgta cgacctcgac gagatcgtca
     3601 acgagtacgt gcgccgcgcc aacggccgct gcaacaccga agtcatccgc tcgttcgtgt
     3661 acaactccgg cgagatgatg gacaacctgg cctcgctgat ccccgacacg tcggacgtgt
     3721 tcgactacga gggcggccag tgcatcgtgc agatcgccta cgacaagccc agcggtgccg
     3781 attaccccgt ggaggtttcg ggctacaaga tgtgggcgtc cacggtgcag acggtgggca
     3841 cgaagaacga gcagcccgtg ggcaagaagc agaagacggg catctcgcgc ctgtccgaaa
     3901 tcgaggaata ctgccgcgac gcggccgagg atctgggcgc cgaatggttc tgcgggcaga
     3961 ccgcggcgcg ctgcgtgcag gatgccgacg gcgctgttaa gggcatcatc gccgaggacg
     4021 ccgacggtaa ctacgtgaag ttcgaggcgg ccaagggcgt catcctggcc acgggcgact
     4081 tcggtggcaa caccgacatg gtgtgggagc tgtgctccga atgcgccgag aacgccgagc
     4141 gccacggcgt ggaccgcgcc gagctcatgg gcatgaccga ctgcgacgga tccggtcata
     4201 agatcgggtg ctgggcgggc ggcgccatcg agagccatcc ccgcccggtt gcgggcgacg
     4261 cgccctccat ctcgttcggc ccttggggct ccacgccgtg tctgtggatg aactgcaacg
     4321 gccagcgctt catgaacgaa tcgttcgcgg gcctcgtgct ggcgcagtcg tgccgccagc
     4381 ctatcaaccc ggaggccaag atggtgggca acttcgccat catggacagc aagtacatga
     4441 gctacatcca ggcgggcggc ctcgatcacg gcgctcccaa ctggggcttc cccgagggca
     4501 ttgaggagtt ccaggcgcag atggcggcgg cggatcccgc tgccggcacg gtggaagtgc
     4561 gcggtttgga gatcgcgaac cgcacgagcc cgttcgtgaa cgagatattc gtgggagcca
     4621 cgctcgaaga cgcgctgaag aacgccggtc tcgaaggcga ggcgctcgag aacgccaagg
     4681 ccagcgtcga gcgctacaac gagctgtgcg ccgcaggcaa ggacaccgac ttcggcaagc
     4741 cggagaacct gctgatcccc atcgacgaag gtccgttctt cgttgccacg cagggcacga
     4801 acaagctgta cggccccggc ctgaacaccc tggcgggctt gtgcgtgaac ggcgattacc
     4861 aggtgctgac cgcttcgaag aacgaggtga tcaagggtct gtatgccgtg ggcaacacga
     4921 tgggcgagcg ctacggcaac gcctacaact gcccctcggc cggcaacaac atgggcaacg
     4981 ctatgaccag cggccgcgtg gccggcaagc acgccgcaac cgcgtagact agtcgactca
     5041 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     5101 gccagattat taatccggct tttttattat ttaattcctg ttataaaaaa aggatcaatt
     5161 ttgaactctc tcccaaagtt gatcccttaa cgatccctgc aggagaaggc catcctgacg
     5221 gatggccttt ttgaagcttt ctagaatcgg gggcccccgg ggggattcta gaacaatgga
     5281 agtcctcgtc tcgccctcga attagcccgc ttaatgagcg ggcttttttt gaattaattc
     5341 tcgcgagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5401 atgtaagtta gcgcgaattg caagctggcc gacgcgctgg gctacgtctt gctggcgttc
     5461 gggagcagaa gagcatacat ctggaagcaa agccaggaaa gcggcctatg gagctgtgcg
     5521 gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc ttttctgagc atggtatttt
     5581 tcatggtatt accaattagc aggaaaataa gccattgaat ataaaagata aaaatgtctt
     5641 gtttacaata gagtgggggg ggtcagcctg ccgccttggg ccgggtgatg tcgtacttgc
     5701 ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag ctccggcaac gcctcgcgca
     5761 cccgctggcg gcgcttgcgc atggtcgaac cactggcctc tgacggccag acatagccgc
     5821 acaaggtatc tatggaagcc ttgccggttt tgccggggtc gatccagcca cacagccgct
     5881 ggtgcagcag gcgggcggtt tcgctgtcca gcgcccgcac ctcgtccatg ctgatgcgca
     5941 catgctggcc gccacccatg acggcctgcg cgatcaaggg gttcagggcc acgtacaggc
     6001 gcccgtccgc ctcgtcgctg gcgtactccg acagcagccg aaacccctgc cgcttgcggc
     6061 cattctgggc gatgatggat accttccaaa ggcgctcgat gcagtcctgt atgtgcttga
     6121 gcgccccacc actatcgacc tctgccccga tttcctttgc cagcgcccga tagctacctt
     6181 tgaccaccat ggcatcagcg gtgacggcct cccacttggg ttccaggaac agccggagct
     6241 gccgtccgcc ttcggtcttg ggttccgggc caagcactag gccattaggc ccagccatgg
     6301 ccaccagccc ttgcaggatg cgcagatcat cagcgcccag cggctccggg ccgctgaact
     6361 cgatccgctt gccgtcgccg tagtcatacg tcacgtccag cttgctgcgc ttgcgctcgc
     6421 cccgcttgag ggcacggaac aggccggggg ccagacagtg cgccgggtcg tgtcggacgt
     6481 ggctgaggct gtgcttgttc ttaggcttca ccacggggca cccccttgct cttgcgctgc
     6541 ctctccagca cggcgggctt gagcaccccg ccgtcatgcc gcctgaacca ccgatcagcg
     6601 aacggtgcgc catagttggc cttgctcaca ccgaagcgga cgaagaaccg gcgctggtcg
     6661 tcgtccacac cccattcctc ggcctcggcg ctggtcatgc tcgacaggta ggactgccag
     6721 cggatgttat cgaccagtac cgagctgccc cggctggcct gctgctggtc gcctgcgccc
     6781 atcatggccg cgcccttgct ggcatggtgc aggaacacga tagagcaccc ggtatcggcg
     6841 gcgatggcct ccatgcgacc gatgacctgg gccatggggc cgctggcgtt ttcttcctcg
     6901 atgtggaacc ggcgcagcgt gtccagcacc atcaggcggc ggccctcggc ggcgcgcttg
     6961 aggccgtcga accactccgg ggccatgatg ttgggcaggc tgccgatcag cggctggatc
     7021 agcaggccgt cagccacggc ttgccgttcc tcggcgctga ggtgcgcccc aagggcgtgc
     7081 aggcggtgat gaatggcggt gggcgggtct tcggcgggca ggtagatcac cgggccggtg
     7141 ggcagttcgc ccacctccag cagatccggc ccgcctgcaa tctgtgcggc cagttgcagg
     7201 gccagcatgg atttaccggc accaccgggc gacaccagcg ccccgaccgt accggccacc
     7261 atgttgggca aaacgtagtc cagcggtggc ggcgctgctg cgaacgcctc cagaatattg
     7321 ataggcttat gggtagccat tgattgcctc ctttgcaggc agttggtggt taggcgctgg
     7381 cggggtcact acccccgccc tgcgccgctc tgagttcttc caggcactcg cgcagcgcct
     7441 cgtattcgtc gtcggtcagc cagaacttgc gctgacgcat ccctttggcc ttcatgcgct
     7501 cggcatatcg cgcttggcgt acagcgtcag ggctggccag caggtcgccg gtctgcttgt
     7561 ccttttggtc tttcatatca gtcaccgaga aacttgccgg ggccgaaagg cttgtcttcg
     7621 cggaacaagg acaaggtgca gccgtcaagg ttaaggctgg ccatatcagc gactgaaaag
     7681 cggccagcct cggccttgtt tgacgtataa ccaaagccac cgggcaacca atagcccttg
     7741 tcacttttga tcaggtagac cgaccctgaa gcgctttttt cgtattccat aaaaccccct
     7801 tctgtgcgtg agtactcata gtataacagg cgtgagtacc aacgcaagca ctacatgctg
     7861 aaatctggcc cgcccctgtc catgcctcgc tggcggggtg ccggtgcccg tgccagctcg
     7921 gcccgcgcaa gctggacgct gggcagaccc atgaccttgc tgacggtgcg ctcgatgtaa
     7981 tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc tggcctcggc catggccttg
     8041 ccgatttcct cggcactgcg gccccggctg gccagcttct gcgcggcgat aaagtcgcac
     8101 ttgctgaggt catcaccgaa gcgcttgacc agcccggcca tctcgctgcg gtactcgtcc
     8161 agcgccgtgc gccggtggcg gctaagctgc cgctcggaca gttcgaggct ggccagcctg
     8221 cgggccttct cctgctgccg ctgggcctgc tcgatctgct ggccagcctg ctgcaccagc
     8281 gccgggccag cggtggcggt cttgcccttg gattcacgca gcagcaccca cggctgataa
     8341 ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg gccatagtgg
     8401 cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg ggcaatctgc
     8461 ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc atgcctgata gttcttcggg
     8521 ctggtttcca ctaccagggc aggctcccgg ccctcggctt tcatgtcatc caggtcaaac
     8581 tcgctgaggt cgtccaccag caccagacca tgccgctcct gctcggcggg cctgatatac
     8641 acgtcattgc cctgggcatt catccgcttg agccatggcg tgttctggag cacttcggcg
     8701 gctgaccatt cccggttcat catctggccg gtggtggcgt ccctgacgcc gatatcgaag
     8761 cgctcacagc ccatggcctt gagctgtcgg cctatggcct gcaaagtcct gtcgttcttc
     8821 atcgggccac caagcgcagc cagatcgagc cgtcctcggt tgtcagtggc gtcaggtcga
     8881 gcaagagcaa cgatgcgatc agcagcacca ccgtaggcat catggaagcc agcatcacgg
     8941 ttagccatag cttccagtgc cacccccgcg acgcgctccg ggcgctctgc gcggcgctgc
     9001 tcacctcggc ggctacctcc cgcaactctt tggccagctc cacccatgcc gcccctgtct
     9061 ggcgctgggc tttcagccac tccgccgcct gcgcctcgct ggcctgctgg gtctggctca
     9121 tgacctgccg ggcttcgtcg gccagtgtcg ccatgctctg ggccagcggt tcgatctgct
     9181 ccgctaactc gttgatgcct ctggatttct tcactctgtc gattgcgttc atggtctatt
     9241 gcctcccggt attcctgtaa gtcgatgatc tgggcgttgg cggtgtcgat gttcagggcc
     9301 acgtctgccc ggtcggtgcg gatgccccgg ccttccatct ccaccacgtt cggccccagg
     9361 tgaacaccgg gcaggcgctc gatgccctgc gcctcaagtg ttctgtggtc aatgcgggcg
     9421 tcgtggcctg cccgctctaa tgcccggttg gcatggtcgg cccatgcctc gcgggtctgc
     9481 tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg ccccgccctt ctccggggtc
     9541 ttgccgttgt accgcttgaa ccactgagcg gcgggccgct cgatgccgtc attgatccgc
     9601 tcggagatca tcaggtggca gtgcgggttc tcgccgccac cggcatggat ggccagcgta
     9661 tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact cggacgccag cgccttctgc
     9721 tggtcgaggg tcagctcgac cggcagggca aattcgacct ccttgaacag ccgcccattg
     9781 gcgcgttcat acaggtcggc agcatcccag tagtcggcgg gccgctcgac gaactccggc
     9841 atgtgcccgg attcggcgtg caagacttca tccatgtcgc gggcatactt gccttcgcgc
     9901 tggatgaagt cggccttggc cctggccgat tggccgcccg acctgctgcc ggttttcgcc
     9961 gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt gcttttcggc tccatgcaat
    10021 ggccctcgga gagcgcaccg cccgaagggt ggccgttagg ccagtttctc gaagagaaac
    10081 cggtaagtgc gccctcccct acaaagtagg gtcgggattg ccgccgctgt gcctccatga
    10141 tagcctacga gacagcacat taacaatggg gtgtcaagat ggttaagggg agcaacaagg
    10201 cggcggatcg gctggccaag ctcgaagaac aacgagcgcg aatcaatgcc gaaattcagc
    10261 gggtgcgggc aagggaacag cagcaagagc gcaagaacga aacaaggcgc aaggtgctgg
    10321 tgggggccat gattttggcc aaggtgaaca gcagcgagtg gccggaggat cggctcatgg
    10381 cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc cttgttcggt ctgccgccac
    10441 gccagaagga tgagccgggc tgaatgatcg accgagacag gccctgcggg gctgcacacg
    10501 cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg ctccagcgta
    10561 tttctgcggg gtttggtgtg gggtttagcg ggctttgccc gcctttcccc ctgccgcgca
    10621 gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca gctatccggc ctctggccgg
    10681 gcatattggg caagggcagc agcgccccac aagggcgctg ataaccgcgc ctagtggatt
    10741 attcttagat aatcatggat ggatttttcc aacaccccgc cagcccccgc ccctgctggg
    10801 tttgcaggtt tgggggcgtg acagttattg caggggttcg tgacagttat tgcagggggg
    10861 cgtgacagtt attgcagggg ttcgtgacag ttagtacggg agtgacgggc actggctggc
    10921 aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc taagcgatag
    10981 actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc ggccctcgcg
    11041 agaattaatt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tagcgatgac
    11101 cctgctgatt ggttcgctga ccgtagactg gttggcttgg tttcatcagc catccgcttg
    11161 ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc
    11221 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
    11281 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
    11341 tggcaaaatc ctgtatatcg tgcgacatcg cgcgcgcgcg cgcggatgct agcggcgcgc
    11401 caaaaaaaag cccgctcatt a
//
