LOCUS       sequence-46478-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins.
  JOURNAL   Mol Cell Biol 1997 Nov;17(11):6448-58.
  PUBMED    9343407
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1997-11"; volume: "17(11)"; pages: "6448-58"
COMMENT     Sequence Label: HOXA9 (murine) HIS-tag pET
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             94..126
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc catatggcta gcatgactgg tggacagcaa
      121 atgggtcggg atcccaccac cggggccctg ggcaactact atgtggactc cttcctgctg
      181 ggcgccgacg ctgctgatga gctgggtgcg ggacgctacg ctccagggac cctgggtcaa
      241 cccccaaggc aggcggcagc tctggccgaa caccccgact tcagtccttg cagcttccag
      301 tccaaggcgg cggtgtttgg tgcctcgtgg aacccagtgc acgcggcggg cgccaatgcg
      361 gtgcctgctg cagtgtatca tcaccaccac cacccctacg tgcatcccca ggcgcccgtg
      421 gcggcggcgg cgccggacgg caggtatatg cgctcctggc tggaacccac gcccggtgcg
      481 ctctccttcg cgggcttacc ctccagccgg ccttatggca ttaaacctga accgctctcg
      541 gccagaaggg gtgactgtcc cacgcttgac actcacactt tgtccctgac tgactatgct
      601 tgtggttctc ctccagttga
//
