LOCUS       sequence-464794-        6911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetic complementation plasmid of LetA(Delta-ZnR-N Delta-ZnR-C) 
            with LetB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Santarossa CC, Li Y, Yousef S, Hasdemir HS, Rodriguez CC, Haase MB, 
            Baek M, Coudray N, Pavek JG, Focke KN, Silverberg AL, Bautista C, 
            Yeh J, Marty MT, Baker D, Tajkhorshid E, Ekiert DC, Bhabha G
  TITLE     LetA defines a structurally distinct transporter family involved in 
            lipid trafficking
  JOURNAL   bioRxiv 2025.03.21.644421
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.21.644421"
COMMENT     Sequence Label: pBEL2792
FEATURES             Location/Qualifiers
     source          1..6911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     62..81
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        62..80
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3930..3948)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3944..3991
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4036..4054)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4122..4226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4227..4295,4296..5087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4445..4464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5258..5846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5747..5766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6000..6017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6032..6171
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6097..6116)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6257..6279
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6273..6464)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatctcga tcccgcgaaa
       61 ttaatacgac tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatataca tatggctctt aacacaccac aaattacgcc gacaaaaaag
      181 ataacagtga gggcaatcgg cgaggaactg ccgcgtagta gtggaggcgg gtctgacggg
      241 cgcgactggt cgctaacgcg cctggcggca atggccttca ccatgctgtt gcttatgccg
      301 tttgcctggg gcgaaccgct gttgcatatc tggctgttag gcattcgtat tgacgccaac
      361 gttatgcaag gcatctggca aatgaccaaa cagggcgatg cgataacggg gtcgatggtc
      421 tttttctgcg ttattggtgc cccgctcatt ctggtgacct ccatagctta tttatggttt
      481 ggtaaccgac tgggaatgaa tttacgtccg gtactgctga tgcttgagcg acttaaagag
      541 tgggtaatgc tcgatatcta cctggtcggc attggcgttg cttctataaa ggtacaggat
      601 tatgcccata tccaggctgg tgtcggcttg ttctcttttg tggcgttggt gattttaacg
      661 acggtgacgt tgtcacatct taatgtcgaa gaactgtggg agcgatttta tccgcagcgc
      721 agtagtggag gcgggtctgg tggcggctcc cgtcgtcgtc atagtctgca aaaatgctgg
      781 gcggcgctgt tagcgtcaat cgttttattg ttacctgcca acctgttgcc tatttctatc
      841 atttatctga acggaggacg gcaggaagat acaattcttt ccggaattat gtcgctggca
      901 agtagcaaca ttgcggttgc aggaatcgtg tttatcgcca gtattctggt cccgtttact
      961 aaagtgatcg tcatgttcac tttactgttg agcattcatt ttaaatgcca gcaaggttta
     1021 cgcacacgca ttctgttact gcggatggtg acctggattg gtcgctggtc gatgctcgac
     1081 ctgtttgtca tatctttaac catgtcgctg attaatcgcg atcagatcct cgcttttact
     1141 atgggaccgg ctgcgtttta tttcggcgca gcggtaattt tgactattct tgctgtggaa
     1201 tggctggaca gccgcttact ttgggatgca catgagtcag gaaacgcccg cttcgacgac
     1261 tgaagcgcag attaaaaata aacgccgtat ctcacctttc tggctgctgc cgttcatcgc
     1321 gctaatgatt gccagttggc tgatttggga cagttatcag gaccggggta ataccgtcac
     1381 catcgacttt atgtcggcgg atggtattgt tccaggccgt acgcctgttc gttatcaggg
     1441 cgttgaagtc ggaacagtgc aggacatcag cctcagcgac gatcttcgta agattgaagt
     1501 caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc gaagagactc agttctggct
     1561 ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg gacgccctcg tcggtgggaa
     1621 ctatatcggc atgatgcccg ggaaaggtaa agagcaggat cactttgtcg cactcgatac
     1681 ccaaccgaaa tatcggctgg acaatggcga tctgatgatc cacctgcaag cccccgatct
     1741 cggttcgctg aacagcggtt cattggtcta tttccgcaag atcccggtgg gaaaagtcta
     1801 cgactatgcc atcaatccca acaagcaagg cgtggtgatt gatgtcctga tcgagcggcg
     1861 ttttaccgat ctggtgaaaa aaggtagccg tttctggaac gtttccggcg ttgatgccaa
     1921 cgtcagtatc agtggcgcga aggtgaaact ggaaagcttg gcggcactgg ttaacggtgc
     1981 gattgccttc gattcaccag aagagtcgaa acctgccgag gcggaagata cctttggtct
     2041 gtatgaagat ctggcccaca gccagcgtgg cgtaataata aaactggaac tgccgagtgg
     2101 ggccggatta accgccgact cgacgccgtt aatgtatcag gggctggaag tcggacagct
     2161 gactaaactg gatttaaatc ctggtggtaa agtcacaggg gaaatgaccg ttgatcccag
     2221 cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta cgcaacccga aattatccct
     2281 tagcgatgct aatctcagcg ccctgctgac cggcaaaacc ttcgagctgg tgcccggcga
     2341 tggcgagcca cgcaaagaat tcgttgttgt gccaggcgaa aaagcactgc tgcatgaacc
     2401 tgatgttctg acgctgaccc tgaccgcgcc ggaaagttac ggtattgatg cgggtcagcc
     2461 gctcattctt cacggcgtgc aggtaggcca ggttatcgat cgtaaactca ccagcaaagg
     2521 cgtcaccttt accgtcgcca tcgagcctca gcatcgcgaa ctggtaaaag gcgatagcaa
     2581 atttgtcgtc aacagccgtg tcgatgtgaa ggtggggctg gatggcgttg agtttctcgg
     2641 tgccagcgcc tcagaatgga tcaatggcgg gatccgtatt ctgccgggcg ataaaggtga
     2701 gatgaaagcc agctatccac tgtatgccaa tttggaaaaa gcgctggaga acagccttag
     2761 cgatttaccc accacaaccg tgagtttgag tgcagagacg ctgccggatg tgcaggcagg
     2821 atcggtagtg ctgtaccgta aatttgaagt tggtgaagtg attaccgtgc gtccgcgagc
     2881 taacgcgttt gacatcgatc tgcatattaa gccggagtat cgcaaccttc tgaccagcaa
     2941 tagcgtgttc tgggcagaag gcggggcgaa agttcagctg aatggtagtg gtctgaccgt
     3001 acaggcatcc ccgctctcca gagctctaaa gggagccatt agcttcgaca acctcagcgg
     3061 tgccagcgcc agtcagcgta aaggcgataa gcgtattctg tatgcttccg aaacagcggc
     3121 ccgtgcggtt ggcgggcaga ttacgcttca cgctttcgat gccggaaaac tggcggtcgg
     3181 gatgccaatt cgctatctcg gtattgacat cgggcaaatc cagacgctgg atctgattac
     3241 cgcacgcaat gaagtacagg caaaggcggt actttacccg gaatatgtcc agacctttgc
     3301 tcgcggtggt acgcgcttct cagtggtcac accgcaaatt tcggcagctg gcgttgagca
     3361 tcttgatact atcctccagc cgtatatcaa cgtcgaacca ggccggggca atcctcgccg
     3421 cgactttgaa ttacaagagg ccaccattac tgattcgcgg tacctggatg gcttaagcat
     3481 tattgttgaa gcgccggaag ccggttcgtt aggcatcggt acgcctgtgc tgttccgtgg
     3541 tctggaagtc ggtacggtta caggaatgac gctggggaca ttgtcagatc gcgtgatgat
     3601 tgcgatgcgc atcagtaaac gctatcaaca cctggtgcgt aacaattccg tcttctggtt
     3661 ggcatcgggt tacagtctgg actttggtct gacgggcggc gtagtgaaaa ccggcacctt
     3721 taaccagttt atccgtggcg gcatcgcctt cgccacgcct ccgggtacgc cactggcacc
     3781 gaaagcccag gaaggcaaac acttcctgtt gcaggaaagt gaaccgaaag agtggcgtga
     3841 atggggaact gcgcttccca aataatgccc acctcgagca gatccggctg ctaacaaagc
     3901 ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg
     3961 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggataatt
     4021 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4081 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4141 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4201 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4261 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4321 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4381 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4441 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     4501 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4561 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4621 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4681 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4741 caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat
     4801 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4861 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4921 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4981 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5041 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5101 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5161 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5221 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5281 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5341 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5401 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5461 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5521 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5581 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5641 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5701 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5761 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5821 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5881 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5941 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6001 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     6061 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     6121 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     6181 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6241 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6301 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     6361 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     6421 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     6481 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     6541 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     6601 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     6661 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     6721 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     6781 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     6841 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     6901 aacgacagga g
//
