LOCUS       sequence-46480-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Liao B, Bao X, Liu L, Feng S, Zovoilis A, Liu W, Xue Y, Cai J, Guo 
            X, Qin B, Zhang R, Wu J, Lai L, Teng M, Niu L, Zhang B, Esteban MA, 
            Pei D
  TITLE     MicroRNA cluster 302-367 enhances somatic cell reprogramming by 
            accelerating a mesenchymal-to-epithelial transition.
  JOURNAL   J Biol Chem. 2011 May 13. 286(19):17359-64.
  PUBMED    21454525
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 May 13. 286(19):17359-64."
COMMENT     Sequence Label: pMXs-MB
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             483..506
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    513..546
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attgctctta cgattagttt tgcagatttg cagttcagcg tatatgtgaa tatatggctg
       61 tgcaaatcca tgcaaaactg attgtgggaa tgtgtacctt tctgcatcgt aatggacacc
      121 tttatgcaca tcttcagcat ccatgcccat tcatccacag gtggggattg gtggcattac
      181 ttgtgttaga tataaagtat tgcacttgtc ccggcctgag gaagaaagag ggtttttaat
      241 cgtcttttag tttctgagta ttgtaagtta tgttagactg taattatgaa atgaactgtt
      301 ttgctgttat caggtggaac acgatgcaat tttggttggt gtaataggag gaaaattgca
      361 cggtatccat ctgtaaaccg caggacctat gtggacagcc gtctatcagt gctatagtaa
      421 gattgtaaaa tcctttagct agtcattgct ttgaacacat ttgggaattc gtatacacta
      481 gtgactacaa ggacgacgat gacaagtgat aaataacttc gtataatgta tgctatacga
      541 agttattcta gtatttaaat gccctcgagc tgactgcggc cgccagcaca gtggtcgacg
      601 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
      661 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
      721 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
      781 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggancagct
      841 gaatatgggn ccaancagga natctgtggt aagcagttcc tgcccccgg
//
