LOCUS       sequence-464810-       15145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The N-terminus of Prp8 was restored thereby destroying the NheI site
            and correcting the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15145)
  TITLE     Hoskins lab plasmids 2025
REFERENCE   2  (bases 1 to 15145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS424 Prp8 WT TRP1
FEATURES             Location/Qualifiers
     source          1..15145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(6930..6947)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7746..7765)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7747..7765)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7775..7792)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7775..7791)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7784..7806)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7932..8387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8019..8038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8229..8250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8490..9164)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(9165..9446)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(9467..9486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9586..9608
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9646..9664)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      9705..11047
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    10037..10084
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        11074..11178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11179..11247,11248..12039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11397..11416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12210..12798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12699..12718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12952..12969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13086..13107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13122..13127,13128..13145,13146..13152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13160..13176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13165..13187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13184..13200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13184..13200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13219..13239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13221..13239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgtgctgaaa gtaggctcaa acccttccga tagcacgttc attatatcct tattttcttc
       61 accccattgg taaccttcgt cggtcaggtt ataagcgctt aatgatactg atcccggggt
      121 ggaaaaaatt gaaatatcaa tgcagtctct tttcttatcc gcaaaaagtt tagaatgagt
      181 tgctacttca gacgcagcca tgaatttaag ttcttctgtc tgggtatgga tccaaccgag
      241 tagttcaagg ccttcagtat ccggaaggtc tccgatgtca gggatgttgc ttatctgaac
      301 ggatcctacg tggcctaatt gaggtaccag cacaacagtc ttgatttctt tcaccttcgg
      361 atgatctttt gctgacatac cataaataaa ggccgccacc tggattttca catcggatat
      421 ctcaataaac ttctttaata aattttttgg aaggacgtaa acattctgtt cctccacgaa
      481 atcatcagcc gaaacataaa tgttttttaa cctcaaatat aatagtgtat tagcaattgc
      541 agattttctc cattcattct ttgaactaaa cgtttgactt tcgtaatcag cggaagctac
      601 tacaactatc tcctcacctt gcgcattgat agttttagtt ttcataacag ttgatgcacc
      661 tgcagcttct tcgtcgttct gcttttctga cctggcagct tctaattcag ccattttttg
      721 tctcttcaca gatggtgcct tgatattttg acccaatata atatctttaa tttccgtttg
      781 agttaaggca gatatattga cattgtattt tctgccgtac tcagtcaaaa tcaaatctct
      841 catttgagat tcgatagtga tccattgttc atcagtgaat gaaggccata aatggtacga
      901 tttgatagtg atcgtggggt cactgagtag aatcattttg gcactttcct cgttcgtctt
      961 taaagctctt aaaagtagtg taagtctaga aaatgcagtg tacgatgaaa tacggtctaa
     1021 ccaatcatca taaatgttaa acagaaccat ctgaggttca gtagctttca tcacaacatc
     1081 agaaagttta tctattgaca ttgcagctga aaatggtagt cgcagctctg ttggcctgat
     1141 tgctatgtta ggaaagtcaa gcatgtgaac ctctaaagga tcaagcattg cctttcttgt
     1201 gacgataatt tgttttggct gctcttcttt gggcaaagat ctcacaagag cactaacttc
     1261 ctcagctgtt ttccatttgg ccaattggct taaacgtttt tgaccggccc atacagaggt
     1321 atggataatt ttcagaaaca gatgcccagt ctttggattt aaagtgaaaa tgcaaccatt
     1381 aattgctttt gtagcaacgt tcccttcaaa agtcttgtgg acagtaactc tatacacatt
     1441 agtgtcgtca acgaagagtt taatgtcgtt attgaataac tcggcatagt ttgaggagtt
     1501 taaaaatggt tcctgtacac tagattgata tatttgaaga ccttttctaa tacgttcacg
     1561 aaggacatat aatgctgggt tagctttcat aattgttctc atactatttt gtatcaaagg
     1621 ttttaatccg ttgaaccaat taccataggc atcatacatg ttataagcca aatcgatacc
     1681 gatcataaca cctgttgggg agggatacat gctgacatta tctgttgtat aatcgaggaa
     1741 ttttgccctc acataacgag atatatcatg agaatcataa tcaccgtatc tcaattgtac
     1801 atcaaaccac attttgttgg taatcaatcc cttaaatgag tcatttgttt catgtaatag
     1861 ggagggtttt gaaacttccc attcatgaac actttccata gttatgtcag cggcagatga
     1921 attcatcttg tacgatttgc ggggatgcac tgtttcttta gtcacagatt caatttgcaa
     1981 aacatctagc tcaccatcga gaatttgaca aatatcaaaa actatacttt catgtatttt
     2041 ttgccacaaa tgagcacgga atatttgaat taaagatatt tttaacgtag gaattttacc
     2101 atggaggaag ataccagtca gatccaattg caccaagaaa ccgacataga catttgctct
     2161 gttaatagtt ggtgaccacc ataaggtaaa tctacgattt ggaatttggc ttaaaccagt
     2221 tctttgagca tgagttagct tcttaaattg catggaatcc tcaaacccgg acgccttttc
     2281 ccaaaacaaa ccttcccatg aattaaaacc agtcccctta aataaagtgt gttccaaaat
     2341 tgtttcaata cctcctaggg cttgaatcac atcagttcta taggcattta aattccataa
     2401 tttaccgtca tggtgggagt ttgtccacca aaaggggctg tttctctcta gagagtactg
     2461 tttaaattcc ctacggattc tgtgacctct gtcatacgct agagtatgtc gatctctttg
     2521 aaataaagtg ctaatacgag gtataccacg atcccacgat ccttcaagtt cttcaaaagc
     2581 taacctccta ttctgctgaa tagcttcctg ccttttcgtt gcatactcgg cccacaccct
     2641 ttgagagtct aaaaattcat tttcccaagt agtgatgtaa cggaagatag ttgggattaa
     2701 tttttcatcc tcgtgcgtca taccagcacg aaaatgcgta attccagtat cggtctgttt
     2761 tgaccaactt aagtcagatg caggaattaa aatatgagaa gcactaatca tacccaaccc
     2821 accaagttct tttggtgtat aaaaaacagc aggtggaaat cttgttggca ttttcgaatt
     2881 taaaccaagt ttgacacggt tttgaattct tgtttctcct ttcaccagga tatctaataa
     2941 cggctccgtg gcaacaatgg cttctctaaa gtatgtgaaa agggatatca gtgaagtgtt
     3001 ccatttagcg gccactttag taaaagtggt tgaaccggag gccatcaata tgcctcttat
     3061 tctgctgtca aattttttga tttcttcttc agataccttt aaatatgctt ttgcagttct
     3121 ttgcttggtt ctttcgtcaa ctaaatccca aacaccttca tcgttagaaa ccacctcttc
     3181 cattctttgt cttggtaata ttctgacttc aaaaccacac atagagaaga gtaagttcgg
     3241 attgttttta ctgtatacgg aaacaaaagc attttcccat ttaattgacg ttaacgaagt
     3301 tggcacccta ctttgaattt cccaaaacac tgctcgccct aggttaacgt cctgacgtat
     3361 tagcctcatg cgcgaatctt tgggccagca ttttctatta ttgtatccga tgctgttttc
     3421 aaagtttgga tctggattct ctgccaagta ttcatcagtc aattcctctc cctcatcttc
     3481 ttcaaagtga aaaagcatat atatacgatc taaatatctg gtgtaaagtc tgatcggatg
     3541 tgccttttct acttctttgc tcttgaattg cataaattca tttggattgt tagctggacc
     3601 agccaaatct gtagcccttt cctgacccaa tagtaaaaga tctataacta gtccgtaata
     3661 ttggaatatg aaagaagcga acttcaaccc gcgtattaag ccatatttgt tgacgtgact
     3721 catatcttta aagttgataa caacattatt ttttgcagtg atatagtcag caatattagg
     3781 atctacaatc aggcgaagta atctattaag taaagtaaag tcaatttttt cggccatttc
     3841 acccaaagta gtctcaagca aaactgcaga ttggcctctg gatacatccc aaatttcaga
     3901 taggttgttt ataccctgag tccacttgta taccagaaga ggtggtatct ctgaatcact
     3961 tggtttaatc cagtttggga aaagctttcg ttggtcagct tcgtaccata aatactgatc
     4021 aagatatgca tcggtaatct tttccaaggg atcaactgaa taaacagggg aaatgttttg
     4081 atagttttcc atcatggtta tatcaacagg cttaaaaact cgctgggtca acaagtactt
     4141 tttgattctg ttcaaagtat cgtgaggatt atcataagcc tcctctatca acgcaagttc
     4201 ttccctttca gatgcattta aacgtacttt tgatgcgtag acatctttta ggtcttcgag
     4261 ggcaagaact aatatcttag tatcattttt gtaagttaaa ggtggaaatg gtattggtga
     4321 aaagctcctt gattcaagcc actcaaccat aacagaaaat atggtcgtag cctcttcggg
     4381 agtaatttcg ggaccgtttt tctgtatttg cctttgacgt tcctgctcat ttttgatcca
     4441 aagtctcgtt aaacgcccca gatttttttt aaccatagtc ttttcgacgt gagctccacg
     4501 ctttatacgt tctcgattgt aatgagctgc agatacccag gcatcagctt tggattttat
     4561 ataccgttca attatttttt tgattggggc tggcatacca ggaacatccc atggaatatt
     4621 agccttccaa catctccaag cctcacttaa atgttgcagt atagttcttg cctttttctg
     4681 tcgaatactc tcaggcatca tttctaaaat gtcatccatg accgagtttc gtaactcaag
     4741 atcgtagtag gcgtcaagtc tttgtttagt tgtagttttg acaatttcat tagaacggcc
     4801 ttcaaattga cgtgtgataa ggtttccaat atatctttcg agtagtggta tagtgcctct
     4861 taagaaattt agccacactc tccacgcagg ttgccaaaat ccacaaccag gtccttttcc
     4921 caaattttta ttaaacttat aatatatgat atgtttcaga tctttacatg cgcggatttg
     4981 atgcattact ttatatttat aacgataaat accggtcaat tgtcctatgt ggttcaaaat
     5041 ataatgtata ccatctgcta actggaaggc atctacattt cctaacctaa actgcacatg
     5101 tgtatctaca atcagtttca tcatttttag caattcacgc atcaaatgaa atgagtttcc
     5161 aagtcttgac tttttacgtt cttttgtggt taaggtttta gttggtttta aattgaaatt
     5221 gtagtcgaga tgtaaataag ttaggccctt tctgtggatt aataaattta acatattgtg
     5281 tccttgtcga catagctgga gtcctgcctc tacccaatca attgtagttt gttgaaaata
     5341 cttggtattt tttaaactct tcaataactt agttttgttg tggtttgttg gcaaagtagg
     5401 gtgcaattca tttagcacgt agttcttcaa gagcttttgg taagagacct taactttaac
     5461 gggatactcc tcgtcaggat gttgcaaaaa ccatttttta gccagggcaa catcttgtgc
     5521 tctcaccatt tttcctttag ttctattaaa tggaaaaggt gaatgatata acgacatcgc
     5581 atcttttgta tttggcaaaa taagttcttc ttcttcagcc aaaagaggtg caaaatcttc
     5641 aggtaaagtg aaatcaccgt tttcctttgt atttgatacg ttgagggatg aattattatc
     5701 aataaagtga ggtattgggt tcagcgatgg atcaaaaaaa agtgcaggcg tgtcgtactc
     5761 ctcatcgttc tggataatac acgacactgg attattatac catggtatgc gcactgaacg
     5821 gggccgggaa ttatatagat gaggaaacgc taccttatat tcgcttctaa taggaaccct
     5881 aaatattact ctatctatag agttgaattc gttataatct tcctcctctt ctcttgggta
     5941 taaaggttca aatttaggac ctccagggat tgcgttatta agggctttac cgttaaaaaa
     6001 agatttttta tcgaaaagat aatagtaatt tttgtcagtt acttcatctc taagtggcgt
     6061 tgataaccta tataagttcg acatttcagg taaatcaaaa ctccactttt tataagatgt
     6121 accattgacc tttttactat cctcctccaa gggccgggaa tcgtagagcc aatctttaac
     6181 atattcatca tcttgagagt ctaagggaag atttatagga tccagtggct cgatattttc
     6241 tatgtgctgt tcatatgaca gaggtggttc gtcatcatcg aaaggtggaa atctcattct
     6301 tttaaaatgt gttctatctc gtttttctct cctcattgct atccaagtag ctgaccattg
     6361 ggcagtatat acaggctcta ttactcttgg tgtttcattc acaaatgtta tggctcctga
     6421 agtgtgatat aaaactttaa cttctttggc ttgctcccat ggatgaggca tgttctctaa
     6481 caattttaaa atcgcgtgcg gtaaatactt tagagcgcct agaaaagcct ttttatcagt
     6541 attgtacatt ttggaggcca tgtcactgtg agtattaata atctttcgca gatgttccgg
     6601 tggcatctcg gctttaggag tatataaatt gcttctcttt gcctttttgg tcattttttt
     6661 tgcacgcttt cttgattttc gagtgacgaa agtatctttt cctatgtcca attttctctt
     6721 tccatgtaat tctactttct tttctgcttt agtctctaat tcatctagac ctggaggggg
     6781 tggtggcggt ggtaatgtga agtccacaat ttcttcagcg tttatttcga agttgcttgg
     6841 aggaggtggt ggaggcggaa ggaatgtatc ctcatttacc gatgatggca ccatcggatt
     6901 atccagttct tcgatttcgt atccaggcgg tggtggtggt ggtggtggaa gtgctaagtc
     6961 gctgtcctct tcaaaaccag gaggtggggg cggtagtcca ctcatctttc tttgacgcta
     7021 tttttttttt ggaaggaagt tcaatcgcac aagtattttc tatggtcttc gtttctcaaa
     7081 ttgtattcct aactttgttg cttttgtact agccgtatta cctcggttca tccttctgcc
     7141 atggtcactt taaatagcta tatagcctgg catttataaa aatttcatat catttagtac
     7201 gaatagctat gtgtatgttc acttcactac atatgccttc tgcactcccg agccagcata
     7261 cgagcctctt caatctcttg agacgttcca ctaggtaccc ccatggcata atcataagcc
     7321 aattggtagt ttcctgcctt aatctcaaat attgccagcc aaagcctagc cttcacggtt
     7381 tcatctgtta ctataccttc cagaagttct tccacatcta cacatttcat catgaacttc
     7441 ttacattctt gcaagtcttc aagttcttca tatagttgcg ctaaccggta atatattgaa
     7501 gtattttgat cgaccgtttg tgaagctttt atggatcttt tgtagcattt tatagcttct
     7561 accttatttc ccgtcttact ataacattct cccaatactt gccaaatccg acgatcccaa
     7621 ggtttcaaag tgcaagcttt ctggaagtag taaagagaat ataaatgcat gtccaggaga
     7681 gcataagcct ggcccaaacc aaaccatgct ttgaagtctc gagggggggc ccggtaccca
     7741 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
     7801 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     7861 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     7921 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7981 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8041 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8101 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8161 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8221 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8281 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8341 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
     8401 tttctcctta cgcatctgtg cggtatttca caccgcatag atcggcaagt gcacaaacaa
     8461 tacttaaata aatactactc agtaataacc tatttcttag catttttgac gaaatttgct
     8521 attttgttag agtcttttac accatttgtc tccacacctc cgcttacatc aacaccaata
     8581 acgccattta atctaagcgc atcaccaaca ttttctggcg tcagtccacc agctaacata
     8641 aaatgtaagc tttcggggct ctcttgcctt ccaacccagt cagaaatcga gttccaatcc
     8701 aaaagttcac ctgtcccacc tgcttctgaa tcaaacaagg gaataaacga atgaggtttc
     8761 tgtgaagctg cactgagtag tatgttgcag tcttttggaa atacgagtct tttaataact
     8821 ggcaaaccga ggaactcttg gtattcttgc cacgactcat ctccatgcag ttggacgata
     8881 tcaatgccgt aatcattgac cagagccaaa acatcctcct taggttgatt acgaaacacg
     8941 ccaaccaagt atttcggagt gcctgaacta tttttatatg cttttacaag acttgaaatt
     9001 ttccttgcaa taaccgggtc aattgttctc tttctattgg gcacacatat aatacccagc
     9061 aagtcagcat cggaatctag agcacattct gcggcctctg tgctctgcaa gccgcaaact
     9121 ttcaccaatg gaccagaact acctgtgaaa ttaataacag acatactcca agctgccttt
     9181 gtgtgcttaa tcacgtatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc
     9241 tctccttttc ttttttcgac cgaattaatt cttaatcggc aaaaaaagaa aagctccgga
     9301 tcaagattgt acgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc
     9361 atctggcgtc ataactgcaa agtacacata tattacgatg ctgttctatt aaatgcttcc
     9421 tatattatat atatagtaat gtcgttgatc tatggtgcac tctcagtaca atctgctctg
     9481 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     9541 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     9601 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     9661 tatttttata ggttaatgtc atgataataa tggtttctta gtatgatcca atatcaaagg
     9721 aaatgatagc attgaaggat gagactaatc caattgagga gtggcagcat atagaacagc
     9781 taaagggtag tgctgaagga agcatacgat accccgcatg gaatgggata atatcacagg
     9841 aggtactaga ctacctttca tcctacataa atagacgcat ataagtacgc atttaagcat
     9901 aaacacgcac tatgccgttc ttctcatgta tatatatata caggcaacac gcagatatag
     9961 gtgcgacgtg aacagtgagc tgtatgtgcg cagctcgcgt tgcattttcg gaagcgctcg
    10021 ttttcggaaa cgctttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
    10081 cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa
    10141 cgcaccggac tgtaacgagc tactaaaata ttgcgaatac cgcttccaca aacattgctc
    10201 aaaagtatct ctttgctata tatctctgtg ctatatccct atataaccta cccatccacc
    10261 tttcgctcct tgaacttgca tctaaactcg acctctacat tttttatgtt tatctctagt
    10321 attactcttt agacaaaaaa attgtagtaa gaactattca tagagtgaat cgaaaacaat
    10381 acgaaaatgt aaacatttcc tatacgtagt atatagagac aaaatagaag aaaccgttca
    10441 taattttctg accaatgaag aatcatcaac gctatcactt tctgttcaca aagtatgcgc
    10501 aatccacatc ggtatagaat ataatcgggg atgcctttat cttgaaaaaa tgcacccgca
    10561 gcttcgctag taatcagtaa acgcgggaag tggagtcagg ctttttttat ggaagagaaa
    10621 atagacacca aagtagcctt cttctaacct taacggacct acagtgcaaa aagttatcaa
    10681 gagactgcat tatagagcgc acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa
    10741 aaatagcgct ctcgggatgc atttttgtag aacaaaaaag aagtatagat tctttgttgg
    10801 taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt
    10861 tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc
    10921 gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct
    10981 ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg
    11041 cttcgttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    11101 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    11161 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    11221 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    11281 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    11341 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    11401 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    11461 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    11521 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    11581 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    11641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    11701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    11761 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    11821 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    11881 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    11941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    12001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    12061 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    12121 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    12181 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    12241 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    12301 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    12361 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    12421 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    12481 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    12541 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    12601 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    12661 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    12721 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    12781 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    12841 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    12901 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    12961 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    13021 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    13081 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    13141 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    13201 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
    13261 cgcggtggcg gccgctctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct
    13321 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc
    13381 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcttca atttctcctc
    13441 tcgattttgt gttatcattc ttcccacaac tttcctgggc tccacttttt tgattggtgt
    13501 tgccttgaat tgttatctga ctctcactct tgcgtttctc aacatacttt tgagttaaaa
    13561 tatctatcaa agagtcatcg ccatcgttgc tatctcctgt tgaatccatt ttatctactt
    13621 cttctgcatt cacctctgct tttttttcaa cgttgggtgc cttgatgggt gaagatggca
    13681 aatcgctgaa tttcgttatc gtcggtttta tagttaaatc gccctctata tcatcgagaa
    13741 tatcatctat attacggaga gactccctgg tagatatttc tgctggttta ggacgcactt
    13801 gctcatttcc attttcttga atgagttgat ttacgtcagc gttgggtact tggatatttg
    13861 tgaaactaag aacagttttc agtcgattag ggactatagg tgtagaagag ggattttgga
    13921 catcttcgta gtcatcagag tattcggcag cgcttgttgt tttgtcggac atatctcgtt
    13981 gtaaaggtga atatttccat attacttctt ccatggggcc gtcctcgtct tgagtgaaat
    14041 cgtgatgcct actcaaatta gacatttccc gtttcggttt aacttgtgaa tttggtaatt
    14101 gctttacgct cttgctaact gctttgttta tatcttttgt tcttgatgta tttcgtactt
    14161 gtgaaacggc tattgacttt aggacacttg cattggatac cttggtgttc ttcccattta
    14221 agttatttat aggagcaaaa gcatactttt ttttcctctt agtttgctta gataatatcg
    14281 cctttgaatc attttgtatt atttcttttt cctctgtctt cttcgcaggt gaaacagata
    14341 tactcgcaga cctcttgttc ttctgtggcg ttccgggcat cctgacgatc tcttcaattg
    14401 tcagtgtttg cttgcacaaa atgagtactc acttgagtat gttttctccc aattttgttt
    14461 tgaggctgcc gtaatgattg taactttatc ttcaaaaatg taacacaagt tttgccataa
    14521 atagcgtaag atcagttaaa tatatataag ttctttctgc cgcacggaat tgctttattg
    14581 cgcgaaatga aaacaagaat atccaatttc tatatgaagg acataatgga atgggagtga
    14641 caacggacca ttttctgtcc gaacgatgtc tatctccttt ggttactttt tgaaatagaa
    14701 tcacagtcaa tgaccaattt tcacatactt atagcttttc atagtgcgga aagcgtagct
    14761 ttgaagtagt tccgaaatta taatacatca tcgtgtggta ggtagcaact acgacgggca
    14821 taaaaaatgc gttgaatatt atacatacaa acttatactt acatatatat ctatgaaata
    14881 acagattcca gtttattggg gaatatattc agctaaatac atcgatttgt tcggcctcta
    14941 actcttcgtc ccccgccaat tcgctaaact gtaaaaagtg tactggacga tgcatctcat
    15001 tatagaattc caaaggaatg ccgtatttaa aattgtagtc accttcttgg ttaaatgctg
    15061 ttcccataaa tgtgtagttc caaacgtttc cagatggaat tataaaatta cctgtgatac
    15121 ggtcagagag aagtaattgg gcatg
//
