LOCUS       sequence-46482-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Alford SC, Abdelfattah AS, Ding Y, Campbell RE
  TITLE     A fluorogenic red fluorescent protein heterodimer.
  JOURNAL   Chem Biol. 2012 Mar 23;19(3):353-60.
  PUBMED    22444590
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem 
            Biol."; date: "2012-03-23"; volume: "19(3)"; pages: "353-60"
COMMENT     Sequence Label: pDDRFP-A1
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             100..132
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(136..144,145..159)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(308..326)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     576..595
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctttttatc gcaactctct actgtttctc catacccgtt ttttgggcta acaggaggaa
       61 ttaaccatgg ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga
      121 cagcaaatgg gtcgggatct gtacgacgat gacgataagg atccgagctc gagaatggtg
      181 agcaagagcg aggaggtcat caaagagttc atgcgcttca aggtgcgctt ggagggctcc
      241 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      301 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
      361 tccccccaga tcatgtacgg ctccaaggcg tacgtgaagc accccgccga cgtccccgat
      421 tacatgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgca cttcgaggac
      481 ggcggtctgg tgaccgtgac tcaggacacc tccctgcagg acggcacgct gatctacaag
      541 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccttg
      601 ggctgggatt atgccaccga gcgcctgtac cccgaagacg gcgtgctgaa gggcgagctt
      661 ctggggcgcc tgaagctgaa ggacggcggc ctcaacctgg tggagttcaa gaccatctac
      721 atggccaaga agcccgtgca actgcccggc tactacttcg tggacaccaa gctgggcatc
      781 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga
//
